| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Activation in Lipopolysaccharide-Induced Airway Inflammation1
Vermont Lung Center and Departments of
*
Medicine and
Pathology, University of Vermont, Burlington, VT 05405
To reveal the causal role of airway epithelial NF-
B activation in evoking airway inflammation, a transgenic mouse was created expressing a mutant version of the inhibitory protein I-B
. This I-B superrepressor (I-BSR) acts to repress NF-B activation exclusively in airway epithelial cells, under the transcriptional control of the rat CC10 promoter (CC10-I-BSR). Compared with transgene-negative littermates, intranasal instillation of LPS did not induce nuclear translocation of NF-B in airway epithelium of CC10-I-BSR transgenic mice. Consequently, the influx of neutrophils into the airways and secretion of the NF-B-regulated neutrophilic chemokine, macrophage-inflammatory protein-2, and the inflammatory cytokine, TNF-, were markedly reduced in CC10-I-BSR mice relative to the transgene-negative mice exposed to LPS. Despite an inability to activate NF-B in airway epithelium, resident alveolar macrophages from transgene-positive mice were capable of activating NF-B in a manner indistinguishable from transgene-negative mice. These findings demonstrate that airway epithelial cells play a prominent role in orchestrating the airway inflammatory response to LPS and suggest that NF-B signaling in these cells is important for modulating innate immune responses to microbial products.
This article has been cited by other articles:
|
|
 |
|
  M. L. Lindauer, J. Wong, Y. Iwakura, and B. E. Magun Pulmonary Inflammation Triggered by Ricin Toxin Requires Macrophages and IL-1 Signaling J. Immunol., July 15, 2009; 183(2): 1419 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Aesif, V. Anathy, M. Havermans, A. S. Guala, K. Ckless, D. J. Taatjes, and Y. M.W. Janssen-Heininger In Situ Analysis of Protein S-Glutathionylation in Lung Tissue Using Glutaredoxin-1-Catalyzed Cysteine Derivatization Am. J. Pathol., July 1, 2009; 175(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. M. W. Janssen-Heininger and A. van der Vliet Oxidants Are Not All Created Equal: Could Hydrogen Peroxide Actually Be Good for Our Lungs? Am. J. Respir. Crit. Care Med., April 15, 2009; 179(8): 627 - 628. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhai, Z. Zhong, C.-Y. A. Chen, Z. Xia, L. Song, M. R. Blackburn, and A.-B. Shyu Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation Mol. Cell. Biol., December 15, 2008; 28(24): 7414 - 7426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Leiva, A. Ruiz-Bravo, and M. Jimenez-Valera Effects of Telithromycin in In Vitro and In Vivo Models of Lipopolysaccharide-Induced Airway Inflammation Chest, July 1, 2008; 134(1): 20 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Kasahara, M. E. Poynter, Z. Othman, D. Hemenway, and A. van der Vliet Acrolein Inhalation Suppresses Lipopolysaccharide-Induced Inflammatory Cytokine Production but Does Not Affect Acute Airways Neutrophilia J. Immunol., July 1, 2008; 181(1): 736 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Ye, J. Ding, X. Zhou, G. Chen, and S. F. Liu Divergent roles of endothelial NF-{kappa}B in multiple organ injury and bacterial clearance in mouse models of sepsis J. Exp. Med., June 9, 2008; 205(6): 1303 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Y. Fong, M. Bebien, A. Didierlaurent, R. Nebauer, T. Hussell, D. Broide, M. Karin, and T. Lawrence An antiinflammatory role for IKK{beta} through the inhibition of "classical" macrophage activation J. Exp. Med., June 9, 2008; 205(6): 1269 - 1276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pantano, J. L. Ather, J. F. Alcorn, M. E. Poynter, A. L. Brown, A. S. Guala, S. L. Beuschel, G. B. Allen, L. A. Whittaker, M. Bevelander, et al. Nuclear Factor-{kappa}B Activation in Airway Epithelium Induces Inflammation and Hyperresponsiveness Am. J. Respir. Crit. Care Med., May 1, 2008; 177(9): 959 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Semaeva, O. Tenstad, A. Bletsa, E.-A. B. Gjerde, and H. Wiig Isolation of rat trachea interstitial fluid and demonstration of local cytokine production in lipopolysaccharide-induced systemic inflammation J Appl Physiol, March 1, 2008; 104(3): 809 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Mizgerd Acute Lower Respiratory Tract Infection N. Engl. J. Med., February 14, 2008; 358(7): 716 - 727. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Haegens, J. H. J. Vernooy, P. Heeringa, B. T. Mossman, and E. F. M. Wouters Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents Eur. Respir. J., February 1, 2008; 31(2): 252 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bevelander, J. Mayette, L. A. Whittaker, S. A. Paveglio, C. C. Jones, J. Robbins, D. Hemenway, S. Akira, S. Uematsu, and M. E. Poynter Nitrogen Dioxide Promotes Allergic Sensitization to Inhaled Antigen J. Immunol., September 15, 2007; 179(6): 3680 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Elizur, T. L. Adair-Kirk, D. G. Kelley, G. L. Griffin, D. E. deMello, and R. M. Senior Clara cells impact the pulmonary innate immune response to LPS Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L383 - L392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Gorska, Q. Liang, S. J. Stafford, N. Goplen, N. Dharajiya, L. Guo, S. Sur, M. Gaestel, and R. Alam MK2 controls the level of negative feedback in the NF-{kappa}B pathway and is essential for vascular permeability and airway inflammation J. Exp. Med., July 9, 2007; 204(7): 1637 - 1652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ckless, A. van der Vliet, and Y. Janssen-Heininger Arginase Modulates NF-{kappa}B Activity via a Nitric Oxide-Dependent Mechanism Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 645 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-s. Cheng, W. Han, S. M. Chen, T. P. Sherrill, M. Chont, G.-Y. Park, J. R. Sheller, V. V. Polosukhin, J. W. Christman, F. E. Yull, et al. Airway Epithelium Controls Lung Inflammation and Injury through the NF-{kappa}B Pathway J. Immunol., May 15, 2007; 178(10): 6504 - 6513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Haegens, T. F. Barrett, J. Gell, A. Shukla, M. MacPherson, P. Vacek, M. E. Poynter, K. J. Butnor, Y. M. Janssen-Heininger, C. Steele, et al. Airway Epithelial NF-{kappa}B Activation Modulates Asbestos-Induced Inflammation and Mucin Production In Vivo J. Immunol., February 1, 2007; 178(3): 1800 - 1808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Quinton, M. R. Jones, B. T. Simms, M. S. Kogan, B. E. Robson, S. J. Skerrett, and J. P. Mizgerd Functions and Regulation of NF-{kappa}B RelA during Pneumococcal Pneumonia J. Immunol., February 1, 2007; 178(3): 1896 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Allard, M. E. Poynter, K. A. Marr, L. Cohn, M. Rincon, and L. A. Whittaker Aspergillus fumigatus Generates an Enhanced Th2-Biased Immune Response in Mice with Defective Cystic Fibrosis Transmembrane Conductance Regulator J. Immunol., October 15, 2006; 177(8): 5186 - 5194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Okutani, M. Lodyga, B. Han, and M. Liu Src protein tyrosine kinase family and acute inflammatory responses Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L129 - L141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Birrell, S. Wong, E. L. Hardaker, M. C. Catley, K. McCluskie, M. Collins, S. Haj-Yahia, and M. G. Belvisi I{kappa}B Kinase-2-Independent and -Dependent Inflammation in Airway Disease Models: Relevance of IKK-2 Inhibition to the Clinic Mol. Pharmacol., June 1, 2006; 69(6): 1791 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Sadikot, H. Zeng, M. Joo, M. B. Everhart, T. P. Sherrill, B. Li, D.-s. Cheng, F. E. Yull, J. W. Christman, and T. S. Blackwell Targeted Immunomodulation of the NF-{kappa}B Pathway in Airway Epithelium Impacts Host Defense against Pseudomonas aeruginosa. J. Immunol., April 15, 2006; 176(8): 4923 - 4930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Knapp, S. Florquin, D. T. Golenbock, and T. van der Poll Pulmonary Lipopolysaccharide (LPS)-Binding Protein Inhibits the LPS-Induced Lung Inflammation In Vivo. J. Immunol., March 1, 2006; 176(5): 3189 - 3195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Regueiro, M. A. Campos, J. Pons, S. Alberti, and J. A. Bengoechea The uptake of a Klebsiella pneumoniae capsule polysaccharide mutant triggers an inflammatory response by human airway epithelial cells Microbiology, February 1, 2006; 152(2): 555 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Neff, B. R. Z'graggen, T. A. Neff, M. Jamnicki-Abegg, D. Suter, R. C. Schimmer, C. Booy, H. Joch, T. Pasch, P. A. Ward, et al. Inflammatory response of tracheobronchial epithelial cells to endotoxin Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L86 - L96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Jones, B. T. Simms, M. M. Lupa, M. S. Kogan, and J. P. Mizgerd Lung NF-{kappa}B Activation and Neutrophil Recruitment Require IL-1 and TNF Receptor Signaling during Pneumococcal Pneumonia J. Immunol., December 1, 2005; 175(11): 7530 - 7535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Noulin, V. F. J. Quesniaux, S. Schnyder-Candrian, B. Schnyder, I. Maillet, T. Robert, B. B. Vargaftig, B. Ryffel, and I. Couillin Both Hemopoietic and Resident Cells Are Required for MyD88-Dependent Pulmonary Inflammatory Response to Inhaled Endotoxin J. Immunol., November 15, 2005; 175(10): 6861 - 6869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Hajjar, H. Harowicz, H. D. Liggitt, P. J. Fink, C. B. Wilson, and S. J. Skerrett An Essential Role for Non-Bone Marrow-Derived Cells in Control of Pseudomonas aeruginosa Pneumonia Am. J. Respir. Cell Mol. Biol., November 1, 2005; 33(5): 470 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, F. Gigliotti, S. Maggirwar, C. Johnston, J. N. Finkelstein, and T. W. Wright Pneumocystis carinii Activates the NF-{kappa}B Signaling Pathway in Alveolar Epithelial Cells Infect. Immun., May 1, 2005; 73(5): 2766 - 2777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Poynter, R. Cloots, T. van Woerkom, K. J. Butnor, P. Vacek, D. J. Taatjes, C. G. Irvin, and Y. M. W. Janssen-Heininger NF-{kappa}B Activation in Airways Modulates Allergic Inflammation but Not Hyperresponsiveness J. Immunol., December 1, 2004; 173(11): 7003 - 7009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.C. dos Santos, B. Han, C.F. Andrade, X. Bai, S. Uhlig, R. Hubmayr, M. Tsang, M. Lodyga, S. Keshavjee, A.S. Slutsky, et al. DNA microarray analysis of gene expression in alveolar epithelial cells in response to TNF{alpha}, LPS, and cyclic stretch Physiol Genomics, November 17, 2004; 19(3): 331 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hjoberg, S. Shore, L. Kobzik, S. Okinaga, A. Hallock, J. Vallone, V. Subramaniam, G. T. De Sanctis, J. A. Elias, J. M. Drazen, et al. Expression of nitric oxide synthase-2 in the lungs decreases airway resistance and responsiveness J Appl Physiol, July 1, 2004; 97(1): 249 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Skerrett, H. D. Liggitt, A. M. Hajjar, R. K. Ernst, S. I. Miller, and C. B. Wilson Respiratory epithelial cells regulate lung inflammation in response to inhaled endotoxin Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L143 - L152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Zou, J. N. Rao, X. Guo, L. Liu, H. M. Zhang, E. D. Strauch, B. L. Bass, and J.-Y. Wang NF-{kappa}B-mediated IAP expression induces resistance of intestinal epithelial cells to apoptosis after polyamine depletion Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1009 - C1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Diehl, T. Krahl, L. Rinaldi, R. Norton, C. G. Irvin, and M. Rincon Inhibition of NFAT Specifically in T Cells Prevents Allergic Pulmonary Inflammation J. Immunol., March 15, 2004; 172(6): 3597 - 3603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Okamoto, K. Gohil, E. I. Finkelstein, P. Bove, T. Akaike, and A. van der Vliet Multiple contributing roles for NOS2 in LPS-induced acute airway inflammation in mice Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L198 - L209. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
