| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pathology, University of Cambridge, Cambridge, United Kingdom
The killer Ig-like receptors (KIR) are a family of highly related MHC class I receptors that show extreme genetic polymorphism both within the human population and between closely related primate species, suggestive of rapid evolutionary diversification. Most KIR are expressed in a variegated fashion by the NK population, giving rise to an NK repertoire of specificities for MHC class I. We compared the promoter for KIR3DL1, which exhibits variegated gene expression, with that for KIR2DL4, which is expressed by all NK cell clones. Maximum transcriptional activity of each was encoded within 
270 bp upstream of the translation initiation codon. The KIR2DL4 promoter drove reporter gene expression only in NK cells, while the KIR3DL1 promoter was active in a range of cell types, suggesting that the latter requires other regulatory elements for physiological expression. In NK cells, reporter gene expression driven by the KIR2DL4 promoter was greater than that driven by the KIR3DL1 promoter. DNase I footprinting revealed that transcription factor binding sites differ between the two promoters. The data indicate that while the promoters of these two KIR genes share 67% nucleotide identity, they have evolved distinct properties consistent with different roles in regulating the generation of NK repertoire.
This article has been cited by other articles:
|
|
 |
|
  G. Li, C. M. Weyand, and J. J. Goronzy Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells J. Leukoc. Biol., September 1, 2008; 84(3): 824 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Thomas, E. Yamada, G. Alter, M. P. Martin, A. A. Bashirova, P. J. Norman, M. Altfeld, P. Parham, S. K. Anderson, D. W. McVicar, et al. Novel KIR3DL1 Alleles and Their Expression Levels on NK Cells: Convergent Evolution of KIR3DL1 Phenotype Variation? J. Immunol., May 15, 2008; 180(10): 6743 - 6750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cooley, F. Xiao, M. Pitt, M. Gleason, V. McCullar, T. L. Bergemann, K. L. McQueen, L. A. Guethlein, P. Parham, and J. S. Miller A subpopulation of human peripheral blood NK cells that lacks inhibitory receptors for self-MHC is developmentally immature Blood, July 15, 2007; 110(2): 578 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Presnell, L. Zhang, C. A. Ramilo, H.-W. Chan, and C. T. Lutz Functional redundancy of transcription factor-binding sites in the killer cell Ig-like receptor (KIR) gene promoter Int. Immunol., August 1, 2006; 18(8): 1221 - 1232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cooley, V. McCullar, R. Wangen, T. L. Bergemann, S. Spellman, D. J. Weisdorf, and J. S. Miller KIR reconstitution is altered by T cells in the graft and correlates with clinical outcomes after unrelated donor transplantation Blood, December 15, 2005; 106(13): 4370 - 4376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-W. Chan, J. S. Miller, M. B. Moore, and C. T. Lutz Epigenetic Control of Highly Homologous Killer Ig-Like Receptor Gene Alleles J. Immunol., November 1, 2005; 175(9): 5966 - 5974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, A. N. Vallejo, Y. Jiang, C. M. Weyand, and J. J. Goronzy Distinct Transcriptional Control Mechanisms of Killer Immunoglobulin-like Receptors in Natural Killer (NK) and in T Cells J. Biol. Chem., June 24, 2005; 280(25): 24277 - 24285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-I. Trompeter, N. Gomez-Lozano, S. Santourlidis, B. Eisermann, P. Wernet, C. Vilches, and M. Uhrberg Three Structurally and Functionally Divergent Kinds of Promoters Regulate Expression of Clonally Distributed Killer Cell Ig-Like Receptors (KIR), of KIR2DL4, and of KIR3DL3 J. Immunol., April 1, 2005; 174(7): 4135 - 4143. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
