| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Microbiology and Immunology, Graduate School of Dentistry and
Laboratory of Biomolecular Function, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
Protease-activated receptors (PARs) compose a family of G protein-coupled receptors activated by proteolysis with exposure of their tethered ligand. Recently, we reported that a neutrophil-derived serine proteinase, proteinase 3 (PR3), activated human oral epithelial cells through PAR-2. The present study examined whether other neutrophil serine proteinases, human leukocyte elastase (HLE), and cathepsin G (Cat G) activate nonepithelial cells, human gingival fibroblasts (HGF). HLE and Cat G as well as PR3 activated HGF to produce IL-8 and monocyte chemoattractant protein 1. Human oral epithelial cells but not HGF express mRNA and protein of secretory leukocyte protease inhibitor, an inhibitor of HLE and Cat G, and recombinant secretory leukocyte protease inhibitor clearly inhibited the activation of HGF induced by HLE and Cat G but not by PR3. HGF express PAR-1 and PAR-2 mRNA in the cells and the proteins on the cell surface. HLE and Cat G cleaved the peptide corresponding to the N terminus of PAR-2 with exposure of its tethered ligand. Treatment with trypsin, an agonist for PAR-2, and a synthetic PAR-2 agonist peptide induced intracellular Ca2+ mobilization and rendered cells refractory to subsequent stimulation with HLE and Cat G. The production of cytokine induced by HLE and Cat G and the PAR-2 agonist peptide was completely abolished by inhibition of phospholipase C. These findings suggest that neutrophil serine proteinases have equal ability to activate human nonepithelial cells through PAR-2 to produce inflammatory cytokines and may control a number of inflammatory processes such as periodontitis.
This article has been cited by other articles:
|
|
 |
|
  L. Atzori, M. Lucattelli, C. J. Scotton, G. J. Laurent, B. Bartalesi, G. De Cunto, B. Lunghi, R. C. Chambers, and G. Lungarella Absence of Proteinase-Activated Receptor-1 Signaling in Mice Confers Protection from fMLP-Induced Goblet Cell Metaplasia Am. J. Respir. Cell Mol. Biol., December 1, 2009; 41(6): 680 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Wilson, K. C. Nannuru, and R. K. Singh Cathepsin G Recruits Osteoclast Precursors via Proteolytic Activation of Protease-Activated Receptor-1 Cancer Res., April 1, 2009; 69(7): 3188 - 3195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Wilson, R. Harada, L. LeDuy, M. D. Hollenberg, and A. Nepveu CUX1 Transcription Factor Is a Downstream Effector of the Proteinase-activated Receptor 2 (PAR2) J. Biol. Chem., January 2, 2009; 284(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Chin, W. Y. Lee, A. Nusrat, N. Vergnolle, and C. A. Parkos Neutrophil-mediated Activation of Epithelial Protease-Activated Receptors-1 and -2 Regulates Barrier Function and Transepithelial Migration J. Immunol., October 15, 2008; 181(8): 5702 - 5710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Uehara, M. Naito, T. Imamura, J. Potempa, J. Travis, K. Nakayama, and H. Takada Dual regulation of interleukin-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalis J. Med. Microbiol., April 1, 2008; 57(4): 500 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kuwahara, E. P. Lillehoj, T. Koga, Y. Isohama, T. Miyata, and K. C. Kim The Signaling Pathway Involved in Neutrophil Elastase Stimulated MUC1 Transcription Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 691 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Dommisch, W. O. Chung, M. G. Rohani, D. Williams, M. Rangarajan, M. A. Curtis, and B. A. Dale Protease-Activated Receptor 2 Mediates Human Beta-Defensin 2 and CC Chemokine Ligand 20 mRNA Expression in Response to Proteases Secreted by Porphyromonas gingivalis Infect. Immun., September 1, 2007; 75(9): 4326 - 4333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Geraghty, M. P. Rogan, C. M. Greene, R. M. M. Boxio, T. Poiriert, M. O'Mahony, A. Belaaouaj, S. J. O'Neill, C. C. Taggart, and N. G. McElvaney Neutrophil Elastase Up-Regulates Cathepsin B and Matrix Metalloprotease-2 Expression J. Immunol., May 1, 2007; 178(9): 5871 - 5878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ramachandran, L. R. Sadofsky, Y. Xiao, A. Botham, M. Cowen, A. H. Morice, and S. J Compton Inflammatory mediators modulate thrombin and cathepsin-G signaling in human bronchial fibroblasts by inducing expression of proteinase-activated receptor-4 Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L788 - L798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Pagano, M. A. Bartoli, T. L. Ennis, D. Mao, P. M. Simmons, R. W. Thompson, and C. T. N. Pham Critical role of dipeptidyl peptidase I in neutrophil recruitment during the development of experimental abdominal aortic aneurysms PNAS, February 20, 2007; 104(8): 2855 - 2860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kida, H. Inoue, T. Shimizu, and K. Kuwano Serratia marcescens Serralysin Induces Inflammatory Responses through Protease-Activated Receptor 2 Infect. Immun., January 1, 2007; 75(1): 164 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kuwahara, E. P. Lillehoj, W. Lu, I. S. Singh, Y. Isohama, T. Miyata, and K. C. Kim Neutrophil elastase induces IL-8 gene transcription and protein release through p38/NF-{kappa}B activation via EGFR transactivation in a lung epithelial cell line Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L407 - L416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Rafiq, M. A. Kolpakov, M. Abdelfettah, D. N. Streblow, A. Hassid, L. J. Dell'Italia, and A. Sabri Role of Protein-tyrosine Phosphatase SHP2 in Focal Adhesion Kinase Down-regulation during Neutrophil Cathepsin G-induced Cardiomyocytes Anoikis J. Biol. Chem., July 14, 2006; 281(28): 19781 - 19792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Csernok, M. Ai, W. L. Gross, D. Wicklein, A. Petersen, B. Lindner, P. Lamprecht, J. U. Holle, and B. Hellmich Wegener autoantigen induces maturation of dendritic cells and licenses them for Th1 priming via the protease-activated receptor-2 pathway Blood, June 1, 2006; 107(11): 4440 - 4448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Camerer, I. Cornelissen, H. Kataoka, D. N. Duong, Y.-W. Zheng, and S. R. Coughlin Roles of protease-activated receptors in a mouse model of endotoxemia Blood, May 15, 2006; 107(10): 3912 - 3921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chignard and D. Pidard Neutrophil and Pathogen Proteinases versus Proteinase-Activated Receptor-2 Lung Epithelial Cells: More Terminators than Activators. Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 394 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Holzhausen, L. C. Spolidorio, R. P. Ellen, M.-C. Jobin, M. Steinhoff, P. Andrade-Gordon, and N. Vergnolle Protease-Activated Receptor-2 Activation: A Major Role in the Pathogenesis of Porphyromonas gingivalis Infection Am. J. Pathol., April 1, 2006; 168(4): 1189 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Kelso, J. C. Lockhart, T. Hembrough, L. Dunning, R. Plevin, M. D. Hollenberg, C. P. Sommerhoff, J. S. McLean, and W. R. Ferrell Therapeutic Promise of Proteinase-Activated Receptor-2 Antagonism in Joint Inflammation J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1017 - 1024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Martin and J. L. Wallace Gastrointestinal Inflammation: A Central Component of Mucosal Defense and Repair Experimental Biology and Medicine, February 1, 2006; 231(2): 130 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hirota, Y. Osuga, T. Hirata, M. Harada, C. Morimoto, O. Yoshino, K. Koga, T. Yano, O. Tsutsumi, and Y. Taketani Activation of protease-activated receptor 2 stimulates proliferation and interleukin (IL)-6 and IL-8 secretion of endometriotic stromal cells Hum. Reprod., December 1, 2005; 20(12): 3547 - 3553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ikawa, T. Nishioka, Z. Yu, Y. Sugawara, J. Kawagoe, T. Takizawa, V. Primo, B. Nikolic, T. Kuroishi, T. Sasano, et al. Involvement of neutrophil recruitment and protease-activated receptor 2 activation in the induction of IL-18 in mice J. Leukoc. Biol., November 1, 2005; 78(5): 1118 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Uehara, K. Muramoto, T. Imamura, K. Nakayama, J. Potempa, J. Travis, S. Sugawara, and H. Takada Arginine-Specific Gingipains from Porphyromonas gingivalis Stimulate Production of Hepatocyte Growth Factor (Scatter Factor) through Protease-Activated Receptors in Human Gingival Fibroblasts in Culture J. Immunol., November 1, 2005; 175(9): 6076 - 6084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Laurent No Bit PARt for PAR-1 Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 213 - 215. [Full Text] [PDF]
|
|
|
|
|
|
T. Suzuki, T. J. Moraes, E. Vachon, H. H. Ginzberg, T.-T. Huang, M. A. Matthay, M. D. Hollenberg, J. Marshall, C. A. G. McCulloch, M. T. H. Abreu, et al. Proteinase-Activated Receptor-1 Mediates Elastase-Induced Apoptosis of Human Lung Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 231 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kuwahara, E. P. Lillehoj, A. Hisatsune, W. Lu, Y. Isohama, T. Miyata, and K. C. Kim Neutrophil elastase stimulates MUC1 gene expression through increased Sp1 binding to the MUC1 promoter Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L355 - L362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tancharoen, K. P. Sarker, T. Imamura, K. K. Biswas, K. Matsushita, S. Tatsuyama, J. Travis, J. Potempa, M. Torii, and I. Maruyama Neuropeptide Release from Dental Pulp Cells by RgpB via Proteinase-Activated Receptor-2 Signaling J. Immunol., May 1, 2005; 174(9): 5796 - 5804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sokolova, Z. Grishina, F. Buhling, T. Welte, and G. Reiser Protease-activated receptor-1 in human lung fibroblasts mediates a negative feedback downregulation via prostaglandin E2 Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L793 - L802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hirota, Y. Osuga, T. Hirata, K. Koga, O. Yoshino, M. Harada, C. Morimoto, E. Nose, T. Yano, O. Tsutsumi, et al. Evidence for the Presence of Protease-Activated Receptor 2 and Its Possible Implication in Remodeling of Human Endometrium J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1662 - 1669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Holzhausen, L.C. Spolidorio, and N. Vergnolle Proteinase-activated Receptor-2 (PAR2) Agonist Causes Periodontitis in Rats Journal of Dental Research, February 1, 2005; 84(2): 154 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Uehara, Y. Sugawara, T. Sasano, H. Takada, and S. Sugawara Proinflammatory Cytokines Induce Proteinase 3 as Membrane-Bound and Secretory Forms in Human Oral Epithelial Cells and Antibodies to Proteinase 3 Activate the Cells through Protease-Activated Receptor-2 J. Immunol., September 15, 2004; 173(6): 4179 - 4189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. S. OSSOVSKAYA and N. W. BUNNETT Protease-Activated Receptors: Contribution to Physiology and Disease Physiol Rev, April 1, 2004; 84(2): 579 - 621. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
