| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110
We isolated dendritic cells (DC) from lymphoid organs of mice bearing a transgene for a membrane-bound form of the model protein hen egg white lysozyme (HEL). DC from the spleen had a lower representation of costimulatory molecules and class II MHC molecules than those isolated from lymph nodes and thymi. Splenic DC were capable of further maturation by in vivo treatment of mice with LPS. The immature DC from spleen processed HEL and displayed the chemically dominant epitope as evidenced by FACS analysis. These immature DC also presented this epitope to CD4+ T cells. Splenic DC from another transgenic mouse (ML-5) containing serum HEL also showed the ability to process and present Ag despite low levels of circulating HEL. In vitro-derived DC from the bone marrow (bone marrow-derived DC) of mHEL mice also displayed immature to mature features and in both cases displayed HEL peptides as well as SDS-stable MHC class II molecules. Immature bone marrow-derived DC also processed exogenous HEL. We conclude that the DC sets normally found in tissue show a scale of maturation features but even the most immature process and present peptides by MHC class II molecules.
This article has been cited by other articles:
|
|
 |
|
  L Salazar, O Aravena, P Abello, A Escobar, J Contreras-Levicoy, N Rojas-Colonelli, D Catalan, A Aguirre, R Zuniga, B Pesce, et al. Modulation of established murine collagen-induced arthritis by a single inoculation of short-term lipopolysaccharide-stimulated dendritic cells Ann Rheum Dis, September 1, 2008; 67(9): 1235 - 1241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Calderon, A. Suri, M. J. Miller, and E. R. Unanue Dendritic cells in islets of Langerhans constitutively present {beta} cell-derived peptides bound to their class II MHC molecules PNAS, April 22, 2008; 105(16): 6121 - 6126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Young, N. S. Wilson, P. Schnorrer, A. Mount, R. J. Lundie, N. L. La Gruta, B. S. Crabb, G. T. Belz, W. R. Heath, and J. A. Villadangos Dendritic cell preactivation impairs MHC class II presentation of vaccines and endogenous viral antigens PNAS, November 6, 2007; 104(45): 17753 - 17758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ireland, J. Herzog, and E. R. Unanue Cutting Edge: Unique T Cells That Recognize Citrullinated Peptides Are a Feature of Protein Immunization J. Immunol., August 1, 2006; 177(3): 1421 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Samuel, K. Whitby, B. C. Keller, A. Marri, W. Barchet, B. R. G. Williams, R. H. Silverman, M. Gale Jr., and M. S. Diamond PKR and RNase L Contribute to Protection against Lethal West Nile Virus Infection by Controlling Early Viral Spread in the Periphery and Replication in Neurons J. Virol., July 15, 2006; 80(14): 7009 - 7019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Shrestha, T. Wang, M. A. Samuel, K. Whitby, J. Craft, E. Fikrig, and M. S. Diamond Gamma Interferon Plays a Crucial Early Antiviral Role in Protection against West Nile Virus Infection. J. Virol., June 1, 2006; 80(11): 5338 - 5348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. F. Shih, J. Racz, and P. M. Allen Differential MHC Class II Presentation of a Pathogenic Autoantigen during Health and Disease J. Immunol., March 15, 2006; 176(6): 3438 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Zwickey, J. J. Unternaehrer, and I. Mellman Presentation of self-antigens on MHC class II molecules during dendritic cell maturation Int. Immunol., January 1, 2006; 18(1): 199 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Herzog, Y. Maekawa, T. P. Cirrito, B. S. Illian, and E. R. Unanue Activated antigen-presenting cells select and present chemically modified peptides recognized by unique CD4 T cells PNAS, May 31, 2005; 102(22): 7928 - 7933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Quah, K. Ni, and H. C. O'Neill In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity Int. Immunol., April 1, 2004; 16(4): 567 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Wilson, D. El-Sukkari, and J. A. Villadangos Dendritic cells constitutively present self antigens in their immature state in vivo and regulate antigen presentation by controlling the rates of MHC class II synthesis and endocytosis Blood, March 15, 2004; 103(6): 2187 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Benvenuti, C. Lagaudriere-Gesbert, I. Grandjean, C. Jancic, C. Hivroz, A. Trautmann, O. Lantz, and S. Amigorena Dendritic Cell Maturation Controls Adhesion, Synapse Formation, and the Duration of the Interactions with Naive T Lymphocytes J. Immunol., January 1, 2004; 172(1): 292 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. El-Sukkari, N. S. Wilson, K. Hakansson, R. J. Steptoe, A. Grubb, K. Shortman, and J. A. Villadangos The Protease Inhibitor Cystatin C Is Differentially Expressed among Dendritic Cell Populations, but Does Not Control Antigen Presentation J. Immunol., November 15, 2003; 171(10): 5003 - 5011. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
