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Selective Regulation of Mature IgG1 Transcription by CD86 and ₂-Adrenergic Receptor Stimulation¹

Department of Molecular Virology, Immunology, and Medical Genetics, Ohio State University, Columbus, OH 43210

Stimulation of CD86 and the ₂-adrenergic receptor (₂AR) on a B cell, either alone or together, is known to increase the level of IgG1 protein produced by a CD40 ligand/IL-4-activated B cell. It is also known that the mechanism by which CD40 and IL-4R stimulation on a B cell increases the level of IgG1 protein is by increasing germline 1 transcription, IgG1 class switching, and mature IgG1 transcription, while the molecular mechanism responsible for mediating the CD86- and ₂AR-induced effect remains unknown. In the present study using real-time PCR we show that the level of mature IgG1 transcription increases in CD40 ligand/IL-4-activated B cells following stimulation of either CD86 and/or ₂AR, and that this increase reflects the increase in IgG1 protein. Furthermore, we show that the CD86- and/or ₂AR-induced increase in mature IgG1 transcript is due to an increase in the rate of mature IgG1 transcription, as determined by nuclear run-on analysis. This effect is additive when both receptors are stimulated and is lost when B cells from CD86- and ₂AR-deficient mice are used. In contrast, the level of germline 1 transcription, the stability of mature IgG1 transcript, the number of IgG1-positive B cells, and the number of IgG1-secreting B cells did not change. These results provide the first evidence that CD86 and/or ₂AR stimulation on a CD40 ligand/IL-4-activated B cell increases the level of IgG1 protein produced per cell by increasing the rate of mature IgG1 transcription.

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