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Department of Biological Sciences, Wayne State University, Detroit, MI 48202
Using high sensitivity fluorescence imaging with shutter speeds
600,000 times faster than those of video frames, we have
characterized Ca2+ waves within cells in exquisite detail
to reveal Ca2+ signaling routes. Polarized neutrophils
exhibited a counterclockwise rotating ryanodine-sensitive juxtamembrane
Ca2+ wave during temporal calcium spikes. During
stimulation with fMLP, a chemotactic factor, two Ca2+ waves
traveling in opposite directions around the perimeter of the cell
emanated from sites of stimulation (the clockwise wave is verapamil
sensitive). Phagocytosed targets exhibit counterclockwise
Ca2+ waves traveling about their periphery originating from
the plasma membrane. This study: 1) outlines the technology to observe
Ca2+ signaling circuitry within small living cells; 2)
shows that extracellular spatial information in the form of a
chemotactic factor gradient is transduced into intracellular chemical
patterns, which provides fresh insights in signaling; 3) suggests that
a line of communication exits between the cell surface and phagosomes;
and 4) suggests that spatiotemporal Ca2+ patterns
contribute to drug actions.
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