HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lamkhioued, B. Articles by Renzi, P. M. Search for Related Content PubMed PubMed Citation Articles by Lamkhioued, B. Articles by Renzi, P. M.

The CCR3 Receptor Is Involved in Eosinophil Differentiation and Is Up-Regulated by Th2 Cytokines in CD34⁺ Progenitor Cells¹

Bouchaib Lamkhioued^2,*,, Soussi Gounni Abdelilah, Qutayba Hamid, Nabil Mansour, Guy Delespesse^* and Paolo M. Renzi^*

^* Centre Hospitalier de l’Universite de Montreal, Research Center, Notre Dame Hospital, University of Montreal, Meakins-Christie Laboratories and Departments of Medicine and Pathology, McGill University, and Charles le Moyne Hospital, Montreal, Quebec, Canada

The involvement of chemokines in eosinophil recruitment during inflammation and allergic reactions is well established. However, a functional role for chemokines in eosinophil differentiation has not been investigated. Using in situ RT-PCR, immunostaining, and flow cytometric analysis, we report that human CD34⁺ cord blood progenitor cells contain CCR3 mRNA and protein. Activation of CD34⁺ progenitor cells under conditions that promote Th2 type differentiation up-regulated surface expression of the CCR3. In contrast, activation with IL-12 and IFN- resulted in a significant decrease in the expression of CCR3. Eotaxin induced Ca²⁺ mobilization in CD34⁺ progenitor cells, which could explain the in vitro and in vivo chemotactic responsiveness to eotaxin. We also found that eotaxin induced the differentiation of eosinophils from cord blood CD34⁺ progenitor cells. The largest number of mature eosinophils was found in cultures containing eotaxin and IL-5. The addition of neutralizing anti-IL-3, anti-IL-5, and anti-GM-CSF Abs to culture medium demonstrated that the differentiation of eosinophils in the presence of eotaxin was IL-3-, IL-5-, and GM-CSF-independent. These results could explain how CD34⁺ progenitor cells accumulate and persist in the airways and peripheral blood of patients with asthma and highlight an alternative mechanism by which blood and tissue eosinophilia might occur in the absence of IL-5.

This article has been cited by other articles:

				N. Bonaros, H. Sondermejer, M. Schuster, R. Rauf, S.F. Wang, T. Seki, D. Skerrett, S. Itescu, and A.A. Kocher CCR3- and CXCR4-mediated interactions regulate migration of CD34+ human bone marrow progenitors to ischemic myocardium and subsequent tissue repair. J. Thorac. Cardiovasc. Surg., October 1, 2008; 136(4): 1044 - 1053. [Abstract] [Full Text] [PDF]

				G. M. Gauvreau, L. P. Boulet, D. W. Cockcroft, A. Baatjes, J. Cote, F. Deschesnes, B. Davis, T. Strinich, K. Howie, M. Duong, et al. Antisense Therapy against CCR3 and the Common Beta Chain Attenuates Allergen-induced Eosinophilic Responses Am. J. Respir. Crit. Care Med., May 1, 2008; 177(9): 952 - 958. [Abstract] [Full Text] [PDF]

				M. B. Rookmaaker, M. C. Verhaar, H. C. de Boer, R. Goldschmeding, J. A. Joles, H. A. Koomans, H.-J. Grone, and T. J. Rabelink Met-RANTES reduces endothelial progenitor cell homing to activated (glomerular) endothelium in vitro and in vivo Am J Physiol Renal Physiol, August 1, 2007; 293(2): F624 - F630. [Abstract] [Full Text] [PDF]

				A. S. Gounni The high-affinity IgE receptor (Fc{epsilon}RI): a critical regulator of airway smooth muscle cells? Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L312 - L321. [Abstract] [Full Text] [PDF]

				F. Huaux, M. Gharaee-Kermani, T. Liu, V. Morel, B. McGarry, M. Ullenbruch, S. L. Kunkel, J. Wang, Z. Xing, and S. H. Phan Role of Eotaxin-1 (CCL11) and CC Chemokine Receptor 3 (CCR3) in Bleomycin-Induced Lung Injury and Fibrosis Am. J. Pathol., December 1, 2005; 167(6): 1485 - 1496. [Abstract] [Full Text] [PDF]

				A. S. Gounni, V. Wellemans, J. Yang, F. Bellesort, K. Kassiri, S. Gangloff, M. Guenounou, A. J. Halayko, Q. Hamid, and B. Lamkhioued Human Airway Smooth Muscle Cells Express the High Affinity Receptor for IgE (Fc{epsilon}RI): A Critical Role of Fc{epsilon}RI in Human Airway Smooth Muscle Cell Function J. Immunol., August 15, 2005; 175(4): 2613 - 2621. [Abstract] [Full Text] [PDF]

				P. Joubert, S. Lajoie-Kadoch, I. Labonte, A. S. Gounni, K. Maghni, V. Wellemans, J. Chakir, M. Laviolette, Q. Hamid, and B. Lamkhioued CCR3 Expression and Function in Asthmatic Airway Smooth Muscle Cells J. Immunol., August 15, 2005; 175(4): 2702 - 2708. [Abstract] [Full Text] [PDF]

				R. G. Boot, M. Verhoek, M. de Fost, C. E. M. Hollak, M. Maas, B. Bleijlevens, M. J. van Breemen, M. van Meurs, L. A. Boven, J. D. Laman, et al. Marked elevation of the chemokine CCL18/PARC in Gaucher disease: a novel surrogate marker for assessing therapeutic intervention Blood, January 1, 2004; 103(1): 33 - 39. [Abstract] [Full Text] [PDF]