| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Signaling Pathway Components1
* Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201;
Departments of Microbiology and Oral Biology, University of Alabama, Birmingham, AL 35294; and
Regulation of Cell Growth Laboratory, National Cancer Institute, Frederick, MD 21702
In this study, tolerance induction by preexposure of murine
macrophages to Toll-like receptor (TLR)2 and TLR4 agonists was
revisited, focusing on the major signaling components associated with
NF-
B activation. Pretreatment of macrophages with a pure TLR4
agonist (protein-free Escherichia coli (Ec) LPS) or with
TLR2 agonists (Porphyromonas gingivalis LPS or synthetic
lipoprotein Pam3Cys) led to suppression of TNF-
secretion,
IL-1R-associated kinase-1, and IB kinase (IKK) kinase
activities, c-jun N-terminal kinase, and extracellular signal-regulated
kinase phosphorylation, and to suppression of NF-B DNA binding and
transactivation upon challenge with the same agonist (TLR4 or TLR2
"homotolerance," respectively). Despite inhibited NF-B DNA
binding, increased levels of nuclear NF-B were detected in
agonist-pretreated macrophages. For all the intermediate signaling
elements, heterotolerance was weaker than TLR4 or TLR2 homotolerance
with the exception of IKK kinase activity. IKK kinase activity was
unperturbed in heterotolerance. TNF- secretion was also suppressed
in P. gingivalis LPS-pretreated, Ec LPS-challenged
cells, but not vice versa, while Pam3Cys and Ec LPS did not induce a
state of cross-tolerance at the level of TNF-. Experiments designed
to elucidate novel mechanisms of NF-B inhibition in tolerized cells
revealed the potential contribution of IB
and IB
inhibitory
proteins and the necessity of TLR4 engagement for induction of
tolerance to Toll receptor-IL-1R domain-containing adapter
protein/MyD88-adapter-like-dependent gene expression. Collectively,
these data demonstrate that induction of homotolerance affects a
broader spectrum of signaling components than in heterotolerance, with
selective modulation of specific elements within the NF-B signaling
pathway.
This article has been cited by other articles:
|
|
 |
|
  P. Gong, D. J. Angelini, S. Yang, G. Xia, A. S. Cross, D. Mann, D. D. Bannerman, S. N. Vogel, and S. E. Goldblum TLR4 Signaling Is Coupled to SRC Family Kinase Activation, Tyrosine Phosphorylation of Zonula Adherens Proteins, and Opening of the Paracellular Pathway in Human Lung Microvascular Endothelia J. Biol. Chem., May 9, 2008; 283(19): 13437 - 13449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Domon, T. Honda, T. Oda, H. Yoshie, and K. Yamazaki Early and preferential induction of IL-1 receptor-associated kinase-M in THP-1 cells by LPS derived from Porphyromonas gingivalis J. Leukoc. Biol., March 1, 2008; 83(3): 672 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Didierlaurent, J. Goulding, S. Patel, R. Snelgrove, L. Low, M. Bebien, T. Lawrence, L. S. van Rijt, B. N. Lambrecht, J.-C. Sirard, et al. Sustained desensitization to bacterial Toll-like receptor ligands after resolutionof respiratory influenza infection J. Exp. Med., February 18, 2008; 205(2): 323 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. A. Ivory, M. Prystajecky, C. Jobin, and K. Chadee Toll-Like Receptor 9-Dependent Macrophage Activation by Entamoeba histolytica DNA Infect. Immun., January 1, 2008; 76(1): 289 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Geisel, F. Kahl, M. Muller, H. Wagner, C. J. Kirschning, I. B. Autenrieth, and J.-S. Frick IL-6 and Maturation Govern TLR2 and TLR4 Induced TLR Agonist Tolerance and Cross-Tolerance in Dendritic Cells J. Immunol., November 1, 2007; 179(9): 5811 - 5818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, M. J. Cowan, J. D. Hasday, S. N. Vogel, and A. E. Medvedev Tobacco Smoking Inhibits Expression of Proinflammatory Cytokines and Activation of IL-1R-Associated Kinase, p38, and NF-{kappa}B in Alveolar Macrophages Stimulated with TLR2 and TLR4 Agonists J. Immunol., November 1, 2007; 179(9): 6097 - 6106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shinohara, K.-i. Hirata, T. Yamashita, T. Takaya, N. Sasaki, R. Shiraki, T. Ueyama, N. Emoto, N. Inoue, M. Yokoyama, et al. Local Overexpression of Toll-Like Receptors at the Vessel Wall Induces Atherosclerotic Lesion Formation: Synergism of TLR2 and TLR4 Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2384 - 2391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Biswas, P. Bist, M. K. Dhillon, T. Kajiji, C. del Fresno, M. Yamamoto, E. Lopez-Collazo, S. Akira, and V. Tergaonkar Role for MyD88-Independent, TRIF Pathway in Lipid A/TLR4-Induced Endotoxin Tolerance J. Immunol., September 15, 2007; 179(6): 4083 - 4092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Kittan, A. Bergua, S. Haupt, N. Donhauser, P. Schuster, K. Korn, T. Harrer, and B. Schmidt Impaired Plasmacytoid Dendritic Cell Innate Immune Responses in Patients with Herpes Virus-Associated Acute Retinal Necrosis J. Immunol., September 15, 2007; 179(6): 4219 - 4230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. J. Roberts, N. Goutagny, P.-Y. Perera, H. Kato, H. Kumar, T. Kawai, S. Akira, R. Savan, D. van Echo, K. A. Fitzgerald, et al. The chemotherapeutic agent DMXAA potently and specifically activates the TBK1-IRF-3 signaling axis J. Exp. Med., July 9, 2007; 204(7): 1559 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Medvedev, W. Piao, J. Shoenfelt, S. H. Rhee, H. Chen, S. Basu, L. M. Wahl, M. J. Fenton, and S. N. Vogel Role of TLR4 Tyrosine Phosphorylation in Signal Transduction and Endotoxin Tolerance J. Biol. Chem., June 1, 2007; 282(22): 16042 - 16053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Yonkers, B. Rodriguez, K. A. Milkovich, R. Asaad, M. M. Lederman, P. S. Heeger, and D. D. Anthony TLR Ligand-Dependent Activation of Naive CD4 T Cells by Plasmacytoid Dendritic Cells Is Impaired in Hepatitis C Virus Infection J. Immunol., April 1, 2007; 178(7): 4436 - 4444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sun, P. E. Fegan, A. S. Desai, J. L. Madara, and M. E. Hobert Flagellin-induced tolerance of the Toll-like receptor 5 signaling pathway in polarized intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G767 - G778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bagchi, E. A. Herrup, H. S. Warren, J. Trigilio, H.-S. Shin, C. Valentine, and J. Hellman MyD88-Dependent and MyD88-Independent Pathways in Synergy, Priming, and Tolerance between TLR Agonists J. Immunol., January 15, 2007; 178(2): 1164 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Buckley, J. H. Wang, and H. P. Redmond Cellular reprogramming by gram-positive bacterial components: a review J. Leukoc. Biol., October 1, 2006; 80(4): 731 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Yoza, J. Y.-Q. Hu, S. L. Cousart, L. M. Forrest, and C. E. McCall Induction of RelB Participates in Endotoxin Tolerance J. Immunol., September 15, 2006; 177(6): 4080 - 4085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Tang, D. Metzger, S. Leeman, and S. Amar LPS-induced TNF-{alpha} factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways PNAS, September 12, 2006; 103(37): 13777 - 13782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Turner, R. S. Langley, K. L. Johnston, G. Egerton, S. Wanji, and M. J. Taylor Wolbachia Endosymbiotic Bacteria of Brugia malayi Mediate Macrophage Tolerance to TLR- and CD40-Specific Stimuli in a MyD88/TLR2-Dependent Manner J. Immunol., July 15, 2006; 177(2): 1240 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Mohammad, M. Morran, B. Slotterbeck, D. W. Leaman, Y. Sun, H. v. Grafenstein, S.-C. Hong, and M. F. McInerney Dysregulated Toll-like receptor expression and signaling in bone marrow-derived macrophages at the onset of diabetes in the non-obese diabetic mouse Int. Immunol., July 1, 2006; 18(7): 1101 - 1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Oak, P. Mandrekar, D. Catalano, K. Kodys, and G. Szabo TLR2- and TLR4-Mediated Signals Determine Attenuation or Augmentation of Inflammation by Acute Alcohol in Monocytes. J. Immunol., June 15, 2006; 176(12): 7628 - 7635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Romics Jr, G. Szabo, J. C. Coffey, J. H. Wang, and H. P. Redmond The Emerging Role of Toll-Like Receptor Pathways in Surgical Diseases Arch Surg, June 1, 2006; 141(6): 595 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Cole, K. L. Elkins, S. M. Michalek, N. Qureshi, L. J. Eaton, P. Rallabhandi, N. Cuesta, and S. N. Vogel Immunologic Consequences of Francisella tularensis Live Vaccine Strain Infection: Role of the Innate Immune Response in Infection and Immunity. J. Immunol., June 1, 2006; 176(11): 6888 - 6899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Li, J. H. Wang, and H. P. Redmond Bacterial lipoprotein-induced self-tolerance and cross-tolerance to LPS are associated with reduced IRAK-1 expression and MyD88-IRAK complex formation J. Leukoc. Biol., April 1, 2006; 79(4): 867 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-T.A. Teng Protective and Destructive Immunity in the Periodontium: Part 2--T-cell-mediated Immunity in the Periodontium. J. Dent. Res., March 1, 2006; 85(3): 209 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou and S. Amar Identification of Proteins Differentially Expressed in Human Monocytes Exposed to Porphyromonas gingivalis and Its Purified Components by High-Throughput Immunoblotting Infect. Immun., February 1, 2006; 74(2): 1204 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Muthukuru and C. W. Cutler Upregulation of Immunoregulatory Src Homology 2 Molecule Containing Inositol Phosphatase and Mononuclear Cell Hyporesponsiveness in Oral Mucosa during Chronic Periodontitis Infect. Immun., February 1, 2006; 74(2): 1431 - 1435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Romics Jr, A. Dolganiuc, A. Velayudham, K. Kodys, P. Mandrekar, D. Golenbock, E. Kurt-Jones, and G. Szabo Toll-like receptor 2 mediates inflammatory cytokine induction but not sensitization for liver injury by Propioni- bacterium acnes J. Leukoc. Biol., December 1, 2005; 78(6): 1255 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Parker, E. C. Jones, L. R. Prince, S. K. Dower, M. K. B. Whyte, and I. Sabroe Endotoxin tolerance induces selective alterations in neutrophil function J. Leukoc. Biol., December 1, 2005; 78(6): 1301 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Cavaillon, C. Adrie, C. Fitting, and M. Adib-Conquy Reprogramming of circulatory cells in sepsis and SIRS Innate Immunity, October 1, 2005; 11(5): 311 - 320. [Abstract] [PDF]
|
|
|
|
|
|
F. J. Quintana and I. R. Cohen Heat Shock Proteins as Endogenous Adjuvants in Sterile and Septic Inflammation J. Immunol., September 1, 2005; 175(5): 2777 - 2782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, G. Li, A. Bafica, M. Pantelic, P. Zhang, H. Broxmeyer, Y. Liu, L. Wetzler, J. J. He, and T. Chen Neisseria gonorrhoeae Enhances Infection of Dendritic Cells by HIV Type 1 J. Immunol., June 15, 2005; 174(12): 7995 - 8002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Hayashi, S. Akira, and A. Nobrega Role of TLR in B Cell Development: Signaling through TLR4 Promotes B Cell Maturation and Is Inhibited by TLR2 J. Immunol., June 1, 2005; 174(11): 6639 - 6647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Perry, C. S. Olver, R. C. Burnett, and A. C. Avery Cutting Edge: The Acquisition of TLR Tolerance during Malaria Infection Impacts T Cell Activation J. Immunol., May 15, 2005; 174(10): 5921 - 5925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Morris, M. K. B. Whyte, G. F. Martin, P. J. Jose, S. K. Dower, and I. Sabroe Agonists of Toll-like Receptors 2 and 4 Activate Airway Smooth Muscle via Mononuclear Leukocytes Am. J. Respir. Crit. Care Med., April 15, 2005; 171(8): 814 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Preciado, E. Caicedo, R. Jhanjee, R. Silver, G. Harris, S. K. Juhn, D. I. Choo, and F. Ondrey Pseudomonas aeruginosa Lipopolysaccharide Induction of Keratinocyte Proliferation, NF-{kappa}B, and Cyclin D1 Is Inhibited by Indomethacin J. Immunol., March 1, 2005; 174(5): 2964 - 2973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Muthukuru, R. Jotwani, and C. W. Cutler Oral Mucosal Endotoxin Tolerance Induction in Chronic Periodontitis Infect. Immun., February 1, 2005; 73(2): 687 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Goodridge, F. A. Marshall, K. J. Else, K. M. Houston, C. Egan, L. Al-Riyami, F.-Y. Liew, W. Harnett, and M. M. Harnett Immunomodulation via Novel Use of TLR4 by the Filarial Nematode Phosphorylcholine-Containing Secreted Product, ES-62 J. Immunol., January 1, 2005; 174(1): 284 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Hatao, M. Muroi, N. Hiki, T. Ogawa, Y. Mimura, M. Kaminishi, and K.-i. Tanamoto Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein J. Leukoc. Biol., October 1, 2004; 76(4): 904 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Siedlar, M. Frankenberger, E. Benkhart, T. Espevik, M. Quirling, K. Brand, M. Zembala, and L. Ziegler-Heitbrock Tolerance Induced by the Lipopeptide Pam3Cys Is Due to Ablation of IL-1R-Associated Kinase-1 J. Immunol., August 15, 2004; 173(4): 2736 - 2745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lorenz, D. C. Chemotti, K. Vandal, and P. A. Tessier Toll-Like Receptor 2 Represses Nonpilus Adhesin-Induced Signaling in Acute Infections with the Pseudomonas aeruginosa pilA Mutant Infect. Immun., August 1, 2004; 72(8): 4561 - 4569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bafica, C. A. Scanga, M. Schito, D. Chaussabel, and A. Sher Influence of Coinfecting Pathogens on HIV Expression: Evidence for a Role of Toll-Like Receptors J. Immunol., June 15, 2004; 172(12): 7229 - 7234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Claud, L. Lu, P. M. Anton, T. Savidge, W. A. Walker, and B. J. Cherayil Developmentally regulated I{kappa}B expression in intestinal epithelium and susceptibility to flagellin-induced inflammation PNAS, May 11, 2004; 101(19): 7404 - 7408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cohen, J. Morisset, and D. Emilie Induction of Tolerance by Porphyromonas gingivalis on APCs: a Mechanism Implicated in Periodontal Infection J. Dent. Res., May 1, 2004; 83(5): 429 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Anthony, N. L. Yonkers, A. B. Post, R. Asaad, F. P. Heinzel, M. M. Lederman, P. V. Lehmann, and H. Valdez Selective Impairments in Dendritic Cell-Associated Function Distinguish Hepatitis C Virus and HIV Infection J. Immunol., April 15, 2004; 172(8): 4907 - 4916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Hongkuan Fan and J. A. Cook Review: Molecular mechanisms of endotoxin tolerance Innate Immunity, April 1, 2004; 10(2): 71 - 84. [Abstract] [PDF]
|
|
|
|
|
|
A. Bafica, C. A. Scanga, O. Equils, and A. Sher The induction of Toll-like receptor tolerance enhances rather than suppresses HIV-1 gene expression in transgenic mice J. Leukoc. Biol., March 1, 2004; 75(3): 460 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kim, B. A. Butcher, and E. Y. Denkers Toxoplasma gondii Interferes with Lipopolysaccharide-Induced Mitogen-Activated Protein Kinase Activation by Mechanisms Distinct from Endotoxin Tolerance J. Immunol., March 1, 2004; 172(5): 3003 - 3010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hajishengallis and R. J. Genco Downregulation of the DNA-Binding Activity of Nuclear Factor-{kappa}B p65 Subunit in Porphyromonas gingivalis Fimbria-Induced Tolerance Infect. Immun., February 1, 2004; 72(2): 1188 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Coats, R. A. Reife, B. W. Bainbridge, T.-T. T. Pham, and R. P. Darveau Porphyromonas gingivalis Lipopolysaccharide Antagonizes Escherichia coli Lipopolysaccharide at Toll-Like Receptor 4 in Human Endothelial Cells Infect. Immun., December 1, 2003; 71(12): 6799 - 6807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Darville, J. M. O'Neill, C. W. Andrews Jr., U. M. Nagarajan, L. Stahl, and D. M. Ojcius Toll-Like Receptor-2, but Not Toll-Like Receptor-4, Is Essential for Development of Oviduct Pathology in Chlamydial Genital Tract Infection J. Immunol., December 1, 2003; 171(11): 6187 - 6197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Flynn, B. K. McFarlin, M. D. Phillips, L. K. Stewart, and K. L. Timmerman Toll-like receptor 4 and CD14 mRNA expression are lower in resistive exercise-trained elderly women J Appl Physiol, November 1, 2003; 95(5): 1833 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Li and B. J. Cherayil Role of Toll-Like Receptor 4 in Macrophage Activation and Tolerance during Salmonella enterica Serovar Typhimurium Infection Infect. Immun., September 1, 2003; 71(9): 4873 - 4882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sabroe, R. C. Read, M. K. B. Whyte, D. H. Dockrell, S. N. Vogel, and S. K. Dower Toll-Like Receptors in Health and Disease: Complex Questions Remain J. Immunol., August 15, 2003; 171(4): 1630 - 1635. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
