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Cutting Edge: Targeting of V to D Rearrangement by RSSs Can Be Mediated by the V(D)J Recombinase in the Absence of Additional Lymphoid-Specific Factors¹

Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110

Assembly of TCR variable region genes is ordered during thymocyte development with D to J rearrangement preceding V to DJ rearrangement. The 5'D 12-RSS is required to precisely and efficiently target V rearrangement beyond simply enforcing the 12/23 rule. By prohibiting direct V to J rearrangement, this restriction ensures D gene segment use in the assembly of essentially all TCR variable region genes. In this study, we show that rearrangement of V 23-RSSs is significantly biased to the D 12-RSS over J 12-RSSs on extrachromosomal recombination substrates in nonlymphoid cells that express the recombinase-activating gene-1/2 proteins. These findings demonstrate that targeting of V to D rearrangement can be enforced by the V(D)J recombinase in the absence of lymphoid-specific factors other than the recombinase-activating gene-1/2 proteins.

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The JI 2003 170: 3-4. [Full Text]  

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