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Identification of Mouse MD-2 Residues Important for Forming the Cell Surface TLR4-MD-2 Complex Recognized by Anti-TLR4-MD-2 Antibodies, and for Conferring LPS and Taxol Responsiveness on Mouse TLR4 by Alanine-Scanning Mutagenesis¹

Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan

The expression of MD-2, which associates with Toll-like receptor (TLR) 4 on the cell surface, confers LPS and LPS-mimetic Taxol responsiveness on TLR4. Alanine-scanning mutagenesis was performed to identify the mouse MD-2 residues important for conferring LPS and Taxol responsiveness on mouse TLR4, and for forming the cell surface TLR4-MD-2 complex recognized by anti-TLR4-MD-2 Ab MTS510. Single alanine mutations were introduced into mouse MD-2 (residues 17–160), and the mutants were expressed in a human cell line expressing mouse TLR4. Mouse MD-2 mutants, in which a single alanine mutation was introduced at Cys³⁷, Leu⁷¹, Leu⁷⁸, Cys⁹⁵, Tyr¹⁰², Cys¹⁰⁵, Glu¹¹¹, Val¹¹³, Ile¹¹⁷, Pro¹¹⁸, Phe¹¹⁹, Glu¹³⁶, Ile¹³⁸, Leu¹⁴⁶, Cys¹⁴⁸, or Thr¹⁵², showed dramatically reduced ability to form the cell surface mouse TLR4-mouse MD-2 complex recognized by MTS510, and the mutants also showed reduced ability to confer LPS and Taxol responsiveness. In contrast, mouse MD-2 mutants, in which a single alanine mutation was introduced at Tyr³⁴, Tyr³⁶, Gly⁵⁹, Val⁸², Ile⁸⁵, Phe¹²⁶, Pro¹²⁷, Gly¹²⁹, Ile¹⁵³, Ile¹⁵⁴, and His¹⁵⁵ showed normal ability to form the cell surface mouse TLR4-mouse MD-2 complex recognized by MTS510, but their ability to confer LPS and Taxol responsiveness was apparently reduced. These results suggest that the ability of MD-2 to form the cell surface mouse TLR4-mouse MD-2 complex recognized by MTS510 is essential for conferring LPS and Taxol responsiveness on TLR4, but not sufficient. In addition, the required residues at codon numbers 34, 85, 101, 122, and 153 for the ability of mouse MD-2 to confer LPS responsiveness are partly different from those for Taxol responsiveness.

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