| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles
* Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717; and
Department of Veterinary Pathobiology, University of Minnesota, St. Paul, MN 55108
Gene expression profiles were compared in circulating bovine
GD3.5+ (CD8-) and GD3.5-
(predominantly CD8+) 
T cells using serial analysis
of gene expression (SAGE). Approximately 20,000 SAGE tags were
generated from each library. A comparison of the two libraries
demonstrated 297 and 173 tags representing genes with 5-fold
differential expression in GD3.5+ and GD3.5-
T cells, respectively. Consistent with their localization into
sites of inflammation, GD3.5+ T cells appeared
transcriptionally and translationally more active than
GD3.5- cells. GD3.5- T cells
demonstrated higher expression of the cell proliferation inhibitor BAP
37, which was associated with their less activated gene expression
phenotype. The immune regulatory and apoptosis-inducing molecule,
galectin-1, was identified as a highly abundant molecule and was higher
in GD3.5+ T cells. Surface molecules attributed to
myeloid cells, such as CD14, CD68, and scavenger receptor-1, were
identified in both populations. Furthermore, expression of B
lymphocyte-induced maturation protein, a master regulator of B cell and
myeloid cell differentiation, was identified by SAGE analysis and was
confirmed at the RNA level to be selectively expressed in T
cells vs 
T cells. These results provide new insights into the
inherent differences between circulating T cell
subsets.
This article has been cited by other articles:
|
|
 |
|
  T. L Davis and J. L Pate Bovine Luteal Cells Stimulate Proliferation of Major Histocompatibility Nonrestricted Gamma Delta T Cells Biol Reprod, December 1, 2007; 77(6): 914 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Lahmers, J. F. Hedges, M. A. Jutila, M. Deng, M. S. Abrahamsen, and W. C. Brown Comparative gene expression by WC1+ {gamma}{delta} and CD4+ {alpha}{beta} T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ {gamma}{delta} T cells. J. Leukoc. Biol., October 1, 2006; 80(4): 939 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Berndt, J. Pieper, and U. Methner Circulating {gamma}{delta} T Cells in Response to Salmonella enterica Serovar Enteritidis Exposure in Chickens Infect. Immun., July 1, 2006; 74(7): 3967 - 3978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Graff, M. Behnke, J. Radke, M. White, and M. A. Jutila A comprehensive SAGE database for the analysis of {gamma}{delta} T cells Int. Immunol., April 1, 2006; 18(4): 613 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Chtanova, R. Newton, S. M. Liu, L. Weininger, T. R. Young, D. G. Silva, F. Bertoni, A. Rinaldi, S. Chappaz, F. Sallusto, et al. Identification of T Cell-Restricted Genes, and Signatures for Different T Cell Responses, Using a Comprehensive Collection of Microarray Datasets J. Immunol., December 15, 2005; 175(12): 7837 - 7847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Hedges, K. J. Lubick, and M. A. Jutila {gamma}{delta} T Cells Respond Directly to Pathogen-Associated Molecular Patterns J. Immunol., May 15, 2005; 174(10): 6045 - 6053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Rogers, D. G. VanBuren, E. E. Hedblom, M. E. Tilahun, J. C. Telfer, and C. L. Baldwin {gamma}{delta} T Cell Function Varies with the Expressed WC1 Coreceptor J. Immunol., March 15, 2005; 174(6): 3386 - 3393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Reverter, S. M. McWilliam, W. Barris, and B. P. Dalrymple A rapid method for computationally inferring transcriptome coverage and microarray sensitivity Bioinformatics, January 1, 2005; 21(1): 80 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Skeen, M. M. Freeman, and H. K. Ziegler Changes in peritoneal myeloid populations and their proinflammatory cytokine expression during infection with Listeria monocytogenes are altered in the absence of {gamma}/{delta} T cells J. Leukoc. Biol., July 1, 2004; 76(1): 104 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Xu, C.-T. Shu, E. Purdom, D. Dang, D. Ilsley, Y. Guo, J. Weber, S. P. Holmes, and P. P. Lee Microarray Analysis Reveals Differences in Gene Expression of Circulating CD8+ T Cells in Melanoma Patients and Healthy Donors Cancer Res., May 15, 2004; 64(10): 3661 - 3667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Hedges, J. C. Graff, and M. A. Jutila Transcriptional Profiling of {gamma}{delta} T Cells J. Immunol., November 15, 2003; 171(10): 4959 - 4964. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
