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RI 
-Chain Gene Expression by GATA-1 Via Four GATA Motifs in the Promoter1
* Atopy (Allergy) Research Center, Juntendo University School of Medicine,
Biotechnology Research Center, University of Tokyo, and
Department of Molecular Cell Immunology and Allergology, Advanced Medical Research, Nihon University School of Medicine, Tokyo, Japan
The FcR 
-chain, a subunit of two related multisubunit receptor
complexes, the Fc
RI and Fc
RIII, amplifies the mast cell response
and is necessary for the cell surface expression of FcRI in mouse.
The transient reporter assay indicated that -69/+4 region is required
for cell type-specific transcriptional regulation of mouse -chain
gene. EMSA using Abs against transcription factors or competitive
oligonucleotides demonstrated that -58/-40 region (containing
overlapping three GATA-1 sites, -53/-48, -46/-51, and -42/-47)
and -31/-26 region (containing one GATA-1 site) are recognized by
GATA-1. The promoter activity of -chain was decreased by nucleotide
replacements of the GATA-1 sites in mouse mast cell line PT18.
Furthermore, exogenously produced GATA-1 up-regulated the promoter
activity in CV-1 cells, which are negative in the -chain production
and the up-regulation was apparently suppressed by GATA-1 site
mutations. These results indicate that cell type-specific transcription
of mouse -chain gene is regulated by GATA-1.
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