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-T Lymphocytes Express and Synthesize Connective Tissue Growth Factor: Effect of IL-15 and TGF-
1 and Comparison with 
-T Lymphocytes1
Pneumology and Allergology, University Medical Clinic IV, Jena, Germany
T lymphocytes bearing the 
-TCR accumulate during wound
healing and inflammation. However, the role of -T lymphocytes in
fibrogenic tissue reactions is not well understood. Therefore, we
addressed the question of whether human -T cells express and
synthesize connective tissue growth factor (CTGF), a factor known to
regulate fibrogenesis and wound healing. In addition, the lymphoblastic
leukemia T cell line (Loucy) that possesses characteristics typical of
-T cells was used as a model to evaluate the regulation of CTGF
gene expression. Blood -T cells isolated from healthy donors were
grown in the presence of IL-15/TGF-
1 for 48 h and assessed for
the expression and synthesis of CTGF. Nonstimulated human blood
-T cells and Loucy -T cells expressed low levels of CTGF
mRNA. Costimulation of the cells with IL-15 and TGF-1 resulted in a
substantially increased level of CTGF mRNA expression within 4–8 h,
and it remained elevated for at least 48 h. In contrast, no CTGF
mRNA was detected when nonstimulated and stimulated human
CD4+ 
-T cells were analyzed. In addition, Western
blot analysis of human -T cell lysates prepared 4 days following
stimulation with IL-15 and TGF-1 revealed a 38-kDa CTGF protein in
cell lysates of human -T cells. Detection was confirmed using
Colo 849 fibroblasts, which can constitutively express high levels of
CTGF. In conclusion, we herein present novel evidence that in contrast
to CD4+ -T cells human -T cells are capable of
expressing CTGF mRNA and synthesizing its corresponding protein, which
supports the concept that -T cells may contribute to wound
healing or tissue fibrotic processes.
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