HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hurst, S. M. Articles by Jones, S. A. Search for Related Content PubMed PubMed Citation Articles by Hurst, S. M. Articles by Jones, S. A. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Substance via MeSH

Secretion of Oncostatin M by Infiltrating Neutrophils: Regulation of IL-6 and Chemokine Expression in Human Mesothelial Cells¹

Suzanne M. Hurst^2,*, Rachel M. McLoughlin^*,, James Monslow^*, Sara Owens, Llinos Morgan, Gerald M. Fuller, Nicholas Topley and Simon A. Jones^*

^* Cardiff School of Biosciences, Cardiff University, Institute of Nephrology, University of Wales College of Medicine, Cardiff, United Kingdom; and Department of Cell Biology, University of Alabama, Birmingham, AL 35294

Recently, we identified that regulation of leukocyte recruitment by IL-6 requires shedding of the IL-6R from infiltrating neutrophils. In this study, experiments have examined whether other IL-6-related cytokines possess similar properties. Levels of oncostatin M (OSM) and leukemia inhibitory factor were analyzed in patients with overt bacterial peritonitis during the first 5 days of infection. Although no change in leukemia inhibitory factor was observed throughout the duration of infection, OSM was significantly elevated on day 1 and rapidly returned to baseline by days 2–3. The source of OSM was identified as the infiltrating neutrophils, and OSM levels correlated both with leukocyte numbers and i.p. soluble IL-6R (sIL-6R) levels. FACS analysis revealed that OSM receptor expression was restricted to human peritoneal mesothelial cells. Stimulation of human peritoneal mesothelial cells with OSM induced phosphorylation of gp130 and OSM receptor , which was accompanied by activation of STAT3 and secretion of CC chemokine ligand 2/monocyte chemoattractant protein-1 and IL-6. Although OSM itself did not modulate CXC chemokine ligand 8/IL-8 release, it effectively suppressed IL-1-mediated expression of this neutrophil-activating CXC chemokine. Moreover, OSM synergistically blocked IL-1-induced CXC chemokine ligand 8 secretion in combination with the IL-6/sIL-6R complex. Thus suggesting that OSM and sIL-6R release from infiltrating neutrophils may contribute to the temporal switch between neutrophil influx and mononuclear cell recruitment seen during acute inflammation.

This article has been cited by other articles:

				E. Larrea, R. Aldabe, I. Gonzalez, V. Segura, P. Sarobe, I. Echeverria, and J. Prieto Oncostatin M Enhances the Antiviral Effects of Type I Interferon and Activates Immunostimulatory Functions in Liver Epithelial Cells J. Virol., April 1, 2009; 83(7): 3298 - 3311. [Abstract] [Full Text] [PDF]

				V. A. Luyckx, L. V. Cairo, C. A. Compston, W. L. Phan, and T. F. Mueller Oncostatin M pathway plays a major role in the renal acute phase response Am J Physiol Renal Physiol, April 1, 2009; 296(4): F875 - F883. [Abstract] [Full Text] [PDF]

				P. G. Tiffen, N. Omidvar, N. Marquez-Almuina, D. Croston, C. J. Watson, and R. W. E. Clarkson A Dual Role for Oncostatin M Signaling in the Differentiation and Death of Mammary Epithelial Cells in Vivo Mol. Endocrinol., December 1, 2008; 22(12): 2677 - 2688. [Abstract] [Full Text] [PDF]

				E. Hams, C. S. Colmont, V. Dioszeghy, V. J. Hammond, C. A. Fielding, A. S. Williams, M. Tanaka, A. Miyajima, P. R. Taylor, N. Topley, et al. Oncostatin M Receptor-{beta} Signaling Limits Monocytic Cell Recruitment in Acute Inflammation J. Immunol., August 1, 2008; 181(3): 2174 - 2180. [Abstract] [Full Text] [PDF]

				C. A. Fielding, R. M. McLoughlin, L. McLeod, C. S. Colmont, M. Najdovska, D. Grail, M. Ernst, S. A. Jones, N. Topley, and B. J. Jenkins IL-6 Regulates Neutrophil Trafficking during Acute Inflammation via STAT3 J. Immunol., August 1, 2008; 181(3): 2189 - 2195. [Abstract] [Full Text] [PDF]

				P. J. Lafontant, A. R. Burns, E. Donnachie, S. B. Haudek, C. W. Smith, and M. L. Entman Oncostatin M differentially regulates CXC chemokines in mouse cardiac fibroblasts Am J Physiol Cell Physiol, July 1, 2006; 291(1): C18 - C26. [Abstract] [Full Text] [PDF]

				M. M. Queen, R. E. Ryan, R. G. Holzer, C. R. Keller-Peck, and C. L. Jorcyk Breast Cancer Cells Stimulate Neutrophils to Produce Oncostatin M: Potential Implications for Tumor Progression Cancer Res., October 1, 2005; 65(19): 8896 - 8904. [Abstract] [Full Text] [PDF]

				C. A. Fielding, R. M. McLoughlin, C. S. Colmont, M. Kovaleva, D. A. Harris, S. Rose-John, N. Topley, and S. A. Jones Viral IL-6 Blocks Neutrophil Infiltration during Acute Inflammation J. Immunol., September 15, 2005; 175(6): 4024 - 4029. [Abstract] [Full Text] [PDF]

				A. Matsukawa, S. Kudo, T. Maeda, K. Numata, H. Watanabe, K. Takeda, S. Akira, and T. Ito Stat3 in Resident Macrophages as a Repressor Protein of Inflammatory Response J. Immunol., September 1, 2005; 175(5): 3354 - 3359. [Abstract] [Full Text] [PDF]

				D. S. Faffe, L. Flynt, M. Mellema, T. R. Whitehead, K. Bourgeois, R. A. Panettieri Jr., E. S. Silverman, and S. A. Shore Oncostatin M causes VEGF release from human airway smooth muscle: synergy with IL-1{beta} Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1040 - L1048. [Abstract] [Full Text] [PDF]

				R. M. McLoughlin, S. M. Hurst, M. A. Nowell, D. A. Harris, S. Horiuchi, L. W. Morgan, T. S. Wilkinson, N. Yamamoto, N. Topley, and S. A. Jones Differential Regulation of Neutrophil-Activating Chemokines by IL-6 and Its Soluble Receptor Isoforms J. Immunol., May 1, 2004; 172(9): 5676 - 5683. [Abstract] [Full Text] [PDF]