| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada
The Ly-49 multigene receptor family regulates mouse NK cell functions. A number of Ly-49 genes exhibit allelic variation, but the functional significance of allelic differences in extracellular domains of Ly-49 receptors regarding ligand specificity is largely unknown. Amino acid differences exist in the extracellular domains of the B6 and BALB/c allele products of the inhibitory Ly-49G receptor. We constructed chimeric Ly-49 receptors consisting of common cytoplasmic and transmembrane regions of the activating Ly-49W receptor fused with the ectodomains of the B6 and BALB/c alleles of Ly-49G. Expression of these chimeras in the RNK-16 rat NK cell line allowed us to study the specificity of inhibitory receptor ectodomains as they stimulated NK lytic activity. We found that the ectodomain of the BALB/c allele of Ly-49G recognizes both H-2Dd and Dk class I MHC alleles, whereas the ectodomain of the B6 allele of Ly-49G recognizes Dd, and not Dk. The specificity for Dk as well as Dd of the wild-type Ly-49GBALB/c allele product was confirmed with RNK-16 transfectants of this inhibitory receptor. Furthermore, the ectodomain of the Ly-49GBALB/c allele recognizes a distinct repertoire of xenogeneic ligands that only partially overlaps with that recognized by Ly-49GB6. Our results indicate that allelic variation in Ly-49 extracellular domains can have functional significance by altering Ly-49 receptor specificity for mouse class I MHC and xenogeneic ligands.
This article has been cited by other articles:
|
|
 |
|
  X. Xie, M. D. Stadnisky, and M. G. Brown MHC Class I Dk Locus and Ly49G2+ NK Cells Confer H-2k Resistance to Murine Cytomegalovirus J. Immunol., June 1, 2009; 182(11): 7163 - 7171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Deng, S. Cho, E. L. Malchiodi, M. C. Kerzic, J. Dam, and R. A. Mariuzza Molecular Architecture of the Major Histocompatibility Complex Class I-binding Site of Ly49 Natural Killer Cell Receptors J. Biol. Chem., June 13, 2008; 283(24): 16840 - 16849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Lavender, H. H. Chau, and K. P. Kane Distinctive Interactions at Multiple Site 2 Subsites by Allele-Specific Rat and Mouse Ly49 Determine Functional Binding and Class I MHC Specificity J. Immunol., November 15, 2007; 179(10): 6856 - 6866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Lavender and K. P. Kane Cross-Species Dependence of Ly49 Recognition on the Supertype Defining B-Pocket of a Class I MHC Molecule J. Immunol., December 15, 2006; 177(12): 8578 - 8586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Osman, E. T. Silver, J. C. Varghese, C. S. Chang, D.-E. Gong, G. F. Audette, B. Hazes, and K. P. Kane Epitope mapping of Ly-49G and G-like receptors: CK-1 antibody defines a polymorphic site of functional interaction with class I ligand J. Leukoc. Biol., May 1, 2005; 77(5): 644 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Lavender, B. J. Ma, E. T. Silver, and K. P. Kane The Rat RT1-A1c MHC Molecule Is a Xenogeneic Ligand Recognized by the Mouse Activating Ly-49W and Inhibitory Ly-49G Receptors J. Immunol., March 15, 2004; 172(6): 3518 - 3526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Makrigiannis, E. Rousselle, and S. K. Anderson Independent Control of Ly49g Alleles: Implications for NK Cell Repertoire Selection and Tumor Cell Killing J. Immunol., February 1, 2004; 172(3): 1414 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Ma, E. T. Silver, B. Hazes, and K. P. Kane Reciprocal Transfer of Class I MHC Allele Specificity between Activating Ly-49P and Ly-49W Receptors by Exchange of {beta}4-{beta}5 Loop Residues J. Immunol., November 15, 2003; 171(10): 5337 - 5344. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
