| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* School of Biomedical and Chemical Sciences,
Western Australian Institute for Medical Research, and
Asthma and Allergy Research Institute and School of Medicine and Pharmacology, University of Western Australia, Crawley, Perth, Western Australia
In previous studies, we demonstrated that allergenic house dust mite proteases are potent inducers of proinflammatory cytokines from the respiratory epithelium, although the precise mechanisms involved were unclear. In this study, we investigated whether this was achieved through activation of protease-activated receptor (PAR)-1 or -2. Pretreatment of A549 respiratory epithelial cells with the clinically important cysteine protease allergen, Der p 1, ablated subsequent PAR-1, but not PAR-2 agonist peptide-induced IL-6 and IL-8 release. HeLa cells transfected with the plasmid coding for PAR-2, in contrast to PAR-1, released significant concentration of IL-6 after exposure to Der p 1. Exposure of HeLa cells transfected with either PAR-1/enhanced yellow fusion protein or PAR-2/enhanced yellow fusion protein to Der p 1 caused receptor internalization in the latter cells only, as judged by confocal microscopy with re-expression of the receptor within 120-min postenzyme exposure. Der p 1-induced cytokine release from both A549 and transfected HeLa cells was accompanied by changes in intracellular Ca2+ concentrations. Desensitization studies showed that Der p 1 pretreatment of the A549 cells resulted in the abolition of both trypsin- and PAR-2 agonist peptide-induced Ca2+ release, but not that induced by subsequent exposure to either thrombin or PAR-1 agonist peptide. These data indicate for the first time that the house dust mite allergen Der p 1-induced cytokine release from respiratory epithelial cells is, in part, mediated by activation of PAR-2, but not PAR-1.
This article has been cited by other articles:
|
|
 |
|
  H. Kouzaki, S. M. O'Grady, C. B. Lawrence, and H. Kita Proteases Induce Production of Thymic Stromal Lymphopoietin by Airway Epithelial Cells through Protease-Activated Receptor-2 J. Immunol., July 15, 2009; 183(2): 1427 - 1434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Shpacovitch, M. Feld, M. D. Hollenberg, T. A. Luger, and M. Steinhoff Role of protease-activated receptors in inflammatory responses, innate and adaptive immunity J. Leukoc. Biol., June 1, 2008; 83(6): 1309 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Vroling, M. J. Jonker, S. Luiten, T. M. Breit, W. J. Fokkens, and C. M. van Drunen Primary Nasal Epithelium Exposed to House Dust Mite Extract Shows Activated Expression in Allergic Individuals Am. J. Respir. Cell Mol. Biol., March 1, 2008; 38(3): 293 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ostrowska, E. Sokolova, and G. Reiser PAR-2 activation and LPS synergistically enhance inflammatory signaling in airway epithelial cells by raising PAR expression level and interleukin-8 release Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1208 - L1218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. H. Heijink, P. M. Kies, H. F. Kauffman, D. S. Postma, A. J. M. van Oosterhout, and E. Vellenga Down-Regulation of E-Cadherin in Human Bronchial Epithelial Cells Leads to Epidermal Growth Factor Receptor-Dependent Th2 Cell-Promoting Activity J. Immunol., June 15, 2007; 178(12): 7678 - 7685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-L. Chiu, D.-W. Perng, C.-H. Yu, S.-N. Su, and L.-P. Chow Mold Allergen, Pen c 13, Induces IL-8 Expression in Human Airway Epithelial Cells by Activating Protease-Activated Receptor 1 and 2 J. Immunol., April 15, 2007; 178(8): 5237 - 5244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. H. Heijink, P. Marcel Kies, A. J. M. van Oosterhout, D. S. Postma, H. F. Kauffman, and E. Vellenga Der p, IL-4, and TGF-beta Cooperatively Induce EGFR-Dependent TARC Expression in Airway Epithelium Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 351 - 359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kida, H. Inoue, T. Shimizu, and K. Kuwano Serratia marcescens Serralysin Induces Inflammatory Responses through Protease-Activated Receptor 2 Infect. Immun., January 1, 2007; 75(1): 164 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Winter, S. S. Shasby, D. R. Ries, and D. M. Shasby PAR2 activation interrupts E-cadherin adhesion and compromises the airway epithelial barrier: protective effect of beta-agonists Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L628 - L635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kikuchi, T. Takai, T. Kuhara, M. Ota, T. Kato, H. Hatanaka, S. Ichikawa, T. Tokura, H. Akiba, K. Mitsuishi, et al. Crucial Commitment of Proteolytic Activity of a Purified Recombinant Major House Dust Mite Allergen Der p1 to Sensitization toward IgE and IgG Responses J. Immunol., August 1, 2006; 177(3): 1609 - 1617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Gu and L.-Y. Lee Hypersensitivity of pulmonary chemosensitive neurons induced by activation of protease-activated receptor-2 in rats J. Physiol., August 1, 2006; 574(3): 867 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Holsapple, D. Jones, T. T. Kawabata, I. Kimber, K. Sarlo, M. K. Selgrade, J. Shah, and M. R. Woolhiser Assessing the Potential to Induce Respiratory Hypersensitivity Toxicol. Sci., May 1, 2006; 91(1): 4 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chignard and D. Pidard Neutrophil and Pathogen Proteinases versus Proteinase-Activated Receptor-2 Lung Epithelial Cells: More Terminators than Activators. Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 394 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Adam, K. K. Hansen, O. F. Astudillo, L. Coulon, F. Bex, X. Duhant, E. Jaumotte, M. D. Hollenberg, and A. Jacquet The House Dust Mite Allergen Der p 1, Unlike Der p 3, Stimulates the Expression of Interleukin-8 in Human Airway Epithelial Cells via a Proteinase-activated Receptor-2-independent Mechanism J. Biol. Chem., March 17, 2006; 281(11): 6910 - 6923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dulon, D. Leduc, G. S. Cottrell, J. D'Alayer, K. K. Hansen, N. W. Bunnett, M. D. Hollenberg, D. Pidard, and M. Chignard Pseudomonas aeruginosa Elastase Disables Proteinase-Activated Receptor 2 in Respiratory Epithelial Cells Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 411 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.C. G. Lee, D. A. Knight, K. B. Lane, D. S. Cheng, M. A. Koay, L. R. Teixeira, J. C. Nesbitt, R. C. Chambers, P. J. Thompson, and R. W. Light Activation of proteinase-activated receptor-2 in mesothelial cells induces pleural inflammation Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L734 - L740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Steinhoff, J. Buddenkotte, V. Shpacovitch, A. Rattenholl, C. Moormann, N. Vergnolle, T. A. Luger, and M. D. Hollenberg Proteinase-Activated Receptors: Transducers of Proteinase-Mediated Signaling in Inflammation and Immune Response Endocr. Rev., February 1, 2005; 26(1): 1 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Cates, R. Fattouh, J. Wattie, M. D. Inman, S. Goncharova, A. J. Coyle, J.-C. Gutierrez-Ramos, and M. Jordana Intranasal Exposure of Mice to House Dust Mite Elicits Allergic Airway Inflammation via a GM-CSF-Mediated Mechanism J. Immunol., November 15, 2004; 173(10): 6384 - 6392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Shpacovitch, G. Varga, A. Strey, M. Gunzer, F. Mooren, J. Buddenkotte, N. Vergnolle, C. P. Sommerhoff, S. Grabbe, V. Gerke, et al. Agonists of proteinase-activated receptor-2 modulate human neutrophil cytokine secretion, expression of cell adhesion molecules, and migration within 3-D collagen lattices J. Leukoc. Biol., August 1, 2004; 76(2): 388 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sakata, K. Arima, T. Takai, W. Sakurai, K. Masumoto, N. Yuyama, Y. Suminami, F. Kishi, T. Yamashita, T. Kato, et al. The Squamous Cell Carcinoma Antigen 2 Inhibits the Cysteine Proteinase Activity of a Major Mite Allergen, Der p 1 J. Biol. Chem., February 13, 2004; 279(7): 5081 - 5087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Johnson, R. E. Wiley, R. Fattouh, F. K. Swirski, B. U. Gajewska, A. J. Coyle, J.-C. Gutierrez-Ramos, R. Ellis, M. D. Inman, and M. Jordana Continuous Exposure to House Dust Mite Elicits Chronic Airway Inflammation and Structural Remodeling Am. J. Respir. Crit. Care Med., February 1, 2004; 169(3): 378 - 385. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
