HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Scamurra, R. W. Articles by Janoff, E. N. Search for Related Content PubMed PubMed Citation Articles by Scamurra, R. W. Articles by Janoff, E. N. Pubmed/NCBI databases Substance via MeSH

Mucosal Plasma Cell Repertoire During HIV-1 Infection¹

Ronald W. Scamurra^*,, Douglas B. Nelson^*,, Xue Mei Lin^*,, Darren J. Miller^*,, Gregg J. Silverman^¶, Tim Kappel^*,, Joseph R. Thurn^*,, Erin Lorenz^*,, Anjali Kulkarni-Narla^*, and Edward N. Janoff^2,*,

^* Mucosal and Vaccine Research Center and Sections of Infectious Disease, Gastroenterology, and Laboratory Medicine and Pathology, Veteran Affairs Medical Center, University of Minnesota School of Medicine, Minneapolis, MN 55417; and ^¶ Department of Medicine, University of California at San Diego, La Jolla, CA 92093

Impaired development of local Ab responses may predispose HIV-1-infected patients to an increased rate, severity, and duration of mucosal infections. We characterized the repertoire of Ig-producing cells in the intestinal effector compartment (the lamina propria) of HIV-1-infected (n = 29) and seronegative control (n = 27) subjects. The density of Ig-producing cells per area was similar in both groups. However, the proportions of IgA-producing cells were lower in both the duodenum and colon from HIV-1-infected patients compared with those of control subjects (p < 0.05), with compensatory increases in IgG-producing cells in the colon and IgM-producing cells in the duodenum. Similarly, among Abs in the lumen the proportions of IgA were also decreased and the proportions of IgG were increased among HIV-1-infected patients. On a molecular level, V_H gene repertoire analyses by RT-PCR revealed comparable proportions of the V_H3 family among duodenal IgA transcripts (50–53%) from both groups. V_H3 expression was decreased only for IgM among patients with advanced HIV-1 disease (n = 6) compared with that of control subjects (n = 8) (48 ± 8 vs 62 ± 13%; p < 0.01). Moreover, the frequencies of individual IgM and IgA V_H3 genes were comparable in each group, including rates of putative HIV-1 gp120-binding V_H3 genes (V3-23, V3-30, V3-30/3-30.5). We conclude that, despite a decrement in local IgA producing cells, the density and molecular V_H repertoire of mucosal plasma cells are relatively intact among patients with HIV-1 infection. These data suggest that HIV-1-infected patients use functional regulatory mechanisms to provide sufficient V_H diversity and effective induction and differentiation of mucosal B cells.

This article has been cited by other articles:

				J.-H. Weitkamp, N. L. Kallewaard, A. L. Bowen, B. J. LaFleur, H. B. Greenberg, and J. E. Crowe Jr VH1-46 Is the Dominant Immunoglobulin Heavy Chain Gene Segment in Rotavirus-Specific Memory B Cells Expressing the Intestinal Homing Receptor {alpha}4{beta}7 J. Immunol., March 15, 2005; 174(6): 3454 - 3460. [Abstract] [Full Text] [PDF]

				D E Nilssen, O Oktedalen, and P Brandtzaeg Intestinal B cell hyperactivity in AIDS is controlled by highly active antiretroviral therapy Gut, April 1, 2004; 53(4): 487 - 493. [Abstract] [Full Text] [PDF]