| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Ligand, Osteoprotegerin, and Receptor Activator of NF-B in Mouse Calvariae1
* Department of Oral Cell Biology, Umea University, Umea, Sweden;
Center for Musculoskeletal Research, National Institute for Working Life, Umea, Sweden;
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205
IL-6, leukemia inhibitory factor (LIF), and oncostatin M (OSM) are
IL-6-type cytokines that stimulate osteoclast formation and function.
In the present study, the resorptive effects of these agents and their
regulation of receptor activator of NF-
B ligand (RANKL), RANK, and
osteoprotegerin (OPG) were studied in neonatal mouse calvaria. When
tested separately, neither human (h) IL-6 nor the human soluble IL-6R
(shIL-6R) stimulated bone resorption, but when hIL-6 and the shIL-6R
were combined, significant stimulation of both mineral and matrix
release from bone explants was noted. Semiquantitative RT-PCR showed
that hIL-6 plus shIL-6R enhanced the expression of RANKL and OPG in
calvarial bones, but decreased RANK expression. Human LIF, hOSM, and
mouse OSM (mOSM) also stimulated 45Ca release and enhanced
the mRNA expression of RANKL and OPG in mouse calvaria, but had no
effect on the expression of RANK. In agreement with the RT-PCR
analyses, ELISA measurements showed that both hIL-6 plus shIL-6R and
mOSM increased RANKL and OPG proteins. 1,25-Dihydroxyvitamin
D3 (D3) also increased the RANKL protein level,
but decreased the protein level of OPG. OPG inhibited 45Ca
release stimulated by RANKL, hIL-6 plus shIL-6R, hLIF, hOSM, mOSM, and
D3. An Ab neutralizing mouse gp130 inhibited
45Ca release induced by hIL-6 plus shIL-6R. These
experiments demonstrated stimulation of calvarial bone resorption and
regulation of mRNA and protein expression of RANKL and OPG by
D3 and IL-6 family cytokines as well as regulation of RANK
expression in preosteoclasts/osteoclasts of mouse calvaria by
D3 and hIL-6 plus shIL-6R.
This article has been cited by other articles:
|
|
 |
|
  J.-M. Dayer and E. Choy Therapeutic targets in rheumatoid arthritis: the interleukin-6 receptor Rheumatology, October 23, 2009; (2009) kep329v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Guise Breaking down bone: new insight into site-specific mechanisms of breast cancer osteolysis mediated by metalloproteinases Genes & Dev., September 15, 2009; 23(18): 2117 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Crook and T. A. Guise RANKL: Targeting Bone and Cancer to Treat Skeletal Complications of Malignancy IBMS BoneKEy, September 1, 2009; 6(9): 323 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Shao, M. Nutu, L. Karlsson-Lindahl, A. Benrick, B. Weijdegard, S. Lager, E. Egecioglu, J. Fernandez-Rodriguez, K. Gemzell-Danielsson, C. Ohlsson, et al. Downregulation of cilia-localized Il-6R{alpha} by 17{beta}-estradiol in mouse and human fallopian tubes Am J Physiol Cell Physiol, July 1, 2009; 297(1): C140 - C151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Onan, E. H. Allan, J. M. W. Quinn, J. H. Gooi, S. Pompolo, N. A. Sims, M. T. Gillespie, and T. J. Martin The Chemokine Cxcl1 Is a Novel Target Gene of Parathyroid Hormone (PTH)/PTH-Related Protein in Committed Osteoblasts Endocrinology, May 1, 2009; 150(5): 2244 - 2253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Kelchtermans, L Geboes, T Mitera, D Huskens, G Leclercq, and P Matthys Activated CD4+CD25+ regulatory T cells inhibit osteoclastogenesis and collagen-induced arthritis Ann Rheum Dis, May 1, 2009; 68(5): 744 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Brounais, C. Chipoy, K. Mori, C. Charrier, S. Battaglia, P. Pilet, C. D. Richards, D. Heymann, F. Redini, and F. Blanchard Oncostatin M Induces Bone Loss and Sensitizes Rat Osteosarcoma to the Antitumor Effect of Midostaurin In vivo Clin. Cancer Res., September 1, 2008; 14(17): 5400 - 5409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Duplomb, M. Baud'huin, C. Charrier, M. Berreur, V. Trichet, F. Blanchard, and D. Heymann Interleukin-6 Inhibits Receptor Activator of Nuclear Factor {kappa}B Ligand-Induced Osteoclastogenesis by Diverting Cells into the Macrophage Lineage: Key Role of Serine727 Phosphorylation of Signal Transducer and Activator of Transcription 3 Endocrinology, July 1, 2008; 149(7): 3688 - 3697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Palmqvist, P. Lundberg, I. Lundgren, L. Hanstrom, and U.H. Lerner IL-1{beta} and TNF-{alpha} Regulate IL-6-type Cytokines in Gingival Fibroblasts Journal of Dental Research, June 1, 2008; 87(6): 558 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-K. Jun, Y.-M. Kang, H.-R. Lee, S.-H. Lee, and B.-K. Choi Highly Conserved Surface Proteins of Oral Spirochetes as Adhesins and Potent Inducers of Proinflammatory and Osteoclastogenic Factors Infect. Immun., June 1, 2008; 76(6): 2428 - 2438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lorenzo, M. Horowitz, and Y. Choi Osteoimmunology: Interactions of the Bone and Immune System Endocr. Rev., June 1, 2008; 29(4): 403 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Nerenz, M. L. Martowicz, and J. W. Pike An Enhancer 20 Kilobases Upstream of the Human Receptor Activator of Nuclear Factor-{kappa}B Ligand Gene Mediates Dominant Activation by 1,25-Dihydroxyvitamin D3 Mol. Endocrinol., May 1, 2008; 22(5): 1044 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Yoshitake, S. Itoh, H. Narita, K. Ishihara, and S. Ebisu Interleukin-6 Directly Inhibits Osteoclast Differentiation by Suppressing Receptor Activator of NF-{kappa}B Signaling Pathways J. Biol. Chem., April 25, 2008; 283(17): 11535 - 11540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Nakchbandi, R. Lang, B. Kinder, and K. L. Insogna The Role of the Receptor Activator of Nuclear Factor-{kappa}B Ligand/Osteoprotegerin Cytokine System in Primary Hyperparathyroidism J. Clin. Endocrinol. Metab., March 1, 2008; 93(3): 967 - 973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Bustamante, X Nogues, L Mellibovsky, L Agueda, S Jurado, E Caceres, J Blanch, R Carreras, A Diez-Perez, D Grinberg, et al. Polymorphisms in the interleukin-6 receptor gene are associated with bone mineral density and body mass index in Spanish postmenopausal women Eur. J. Endocrinol., November 1, 2007; 157(5): 677 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamaguchi, N. Aihara, T. Kojima, and K. Kasai RANKL Increase in Compressed Periodontal Ligament Cells from Root Resorption Journal of Dental Research, August 1, 2006; 85(8): 751 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U.H. Lerner Bone Remodeling in Post-menopausal Osteoporosis Journal of Dental Research, July 1, 2006; 85(7): 584 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U.H. Lerner Inflammation-induced Bone Remodeling in Periodontal Disease and the Influence of Post-menopausal Osteoporosis Journal of Dental Research, July 1, 2006; 85(7): 596 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Medicherla, J. Y. Ma, R. Mangadu, Y. Jiang, J. J. Zhao, R. Almirez, I. Kerr, E. G. Stebbins, G. O'Young, A. M. Kapoun, et al. A Selective p38{alpha} Mitogen-Activated Protein Kinase Inhibitor Reverses Cartilage and Bone Destruction in Mice with Collagen-Induced Arthritis J. Pharmacol. Exp. Ther., July 1, 2006; 318(1): 132 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Swanson, M. Lorentzon, H. H. Conaway, and U. H. Lerner Glucocorticoid Regulation of Osteoclast Differentiation and Expression of Receptor Activator of Nuclear Factor-{kappa}B (NF-{kappa}B) Ligand, Osteoprotegerin, and Receptor Activator of NF-{kappa}B in Mouse Calvarial Bones Endocrinology, July 1, 2006; 147(7): 3613 - 3622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dovio, L. Perazzolo, L. Saba, A. Termine, M. Capobianco, A. Bertolotto, and A. Angeli High-dose glucocorticoids increase serum levels of soluble IL-6 receptor {alpha} and its ratio to soluble gp130: an additional mechanism for early increased bone resorption. Eur. J. Endocrinol., May 1, 2006; 154(5): 745 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Palmqvist, P. Lundberg, E. Persson, A. Johansson, I. Lundgren, A. Lie, H. H. Conaway, and U. H. Lerner Inhibition of Hormone and Cytokine-stimulated Osteoclastogenesis and Bone Resorption by Interleukin-4 and Interleukin-13 Is Associated with Increased Osteoprotegerin and Decreased RANKL and RANK in a STAT6-dependent Pathway J. Biol. Chem., February 3, 2006; 281(5): 2414 - 2429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Tabibiazar, R. A. Wagner, E. A. Ashley, J. Y. King, R. Ferrara, J. M. Spin, D. A. Sanan, B. Narasimhan, R. Tibshirani, P. S. Tsao, et al. Signature patterns of gene expression in mouse atherosclerosis and their correlation to human coronary disease Physiol Genomics, July 14, 2005; 22(2): 213 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cui, T. Nomura, H. Noma, K. Yokoo, R. Takagi, S. Hashimoto, M. Okamoto, M. Sato, G. Yu, C. Guo, et al. Effect of YM529 on a Model of Mandibular Invasion by Oral Squamous Cell Carcinoma in Mice Clin. Cancer Res., April 1, 2005; 11(7): 2713 - 2719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Lee, K.-K. Kim, and B.-K. Choi Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum, the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections Infect. Immun., January 1, 2005; 73(1): 268 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Golledge, M. McCann, S. Mangan, A. Lam, and M. Karan Osteoprotegerin and Osteopontin Are Expressed at High Concentrations Within Symptomatic Carotid Atherosclerosis Stroke, July 1, 2004; 35(7): 1636 - 1641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. H. Lerner NEW MOLECULES IN THE TUMOR NECROSIS FACTOR LIGAND AND RECEPTOR SUPERFAMILIES WITH IMPORTANCE FOR PHYSIOLOGICAL AND PATHOLOGICAL BONE RESORPTION Critical Reviews in Oral Biology & Medicine, March 1, 2004; 15(2): 64 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takuma, T. Kaneda, T. Sato, S. Ninomiya, M. Kumegawa, and Y. Hakeda Dexamethasone Enhances Osteoclast Formation Synergistically with Transforming Growth Factor-{beta} by Stimulating the Priming of Osteoclast Progenitors for Differentiation into Osteoclasts J. Biol. Chem., November 7, 2003; 278(45): 44667 - 44674. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
