HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Naeher, D. Articles by Palmer, E. Search for Related Content PubMed PubMed Citation Articles by Naeher, D. Articles by Palmer, E.

A Role for the -Chain Connecting Peptide Motif in Mediating TCR-CD8 Cooperation

Dieter Naeher^*, Immanuel F. Luescher and Ed Palmer^1,*

^* Laboratory of Transplantation Immunology and Nephrology, University Hospital, Basel, Switzerland; and Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Epalinges, Switzerland

To generate peripheral T cells that are both self-MHC restricted and self-MHC tolerant, thymocytes are subjected to positive and negative selection. How the TCR discriminates between positive and negative selection ligands is not well understood, although there is substantial evidence that the CD4 and CD8 coreceptors play an important role in this cell fate decision. We have previously identified an evolutionarily conserved motif in the TCR, the -chain connecting peptide motif (-CPM), which allows the TCR to deliver positive selection signals. Thymocytes expressing -CPM-deficient receptors do not undergo positive selection, whereas their negative selection is not impaired. In this work we studied the ligand binding and receptor function of -CPM-deficient TCRs by generating T cell hybridomas expressing wild-type or -CPM-deficient forms of the T1 TCR. This K^d-restricted TCR is specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide_252–260 IASA-YIPSAEK(ABA)I and is therefore amenable to TCR photoaffinity labeling. The experiments presented in this work show that -CPM-deficient TCRs fail to cooperate with CD8 to enhance ligand binding and functional responses.

This article has been cited by other articles:

				G. Sarikonda, H. Wang, K.-J. Puan, X.-h. Liu, H. K. Lee, Y. Song, M. D. Distefano, E. Oldfield, G. D. Prestwich, and C. T. Morita Photoaffinity Antigens for Human {gamma}{delta} T Cells J. Immunol., December 1, 2008; 181(11): 7738 - 7750. [Abstract] [Full Text] [PDF]

				J. C. Varghese and K. P. Kane TCR Complex-Activated CD8 Adhesion Function by Human T Cells J. Immunol., November 1, 2008; 181(9): 6002 - 6009. [Abstract] [Full Text] [PDF]

				M. Mallaun, D. Naeher, M. A. Daniels, P. P. Yachi, B. Hausmann, I. F. Luescher, N. R. J. Gascoigne, and E. Palmer The T Cell Receptor's {alpha}-Chain Connecting Peptide Motif Promotes Close Approximation of the CD8 Coreceptor Allowing Efficient Signal Initiation J. Immunol., June 15, 2008; 180(12): 8211 - 8221. [Abstract] [Full Text] [PDF]

				Z. Xiao, M. F. Mescher, and S. C. Jameson Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation J. Exp. Med., October 29, 2007; 204(11): 2667 - 2677. [Abstract] [Full Text] [PDF]

				L. Wooldridge, H. A. van den Berg, M. Glick, E. Gostick, B. Laugel, S. L. Hutchinson, A. Milicic, J. M. Brenchley, D. C. Douek, D. A. Price, et al. Interaction between the CD8 Coreceptor and Major Histocompatibility Complex Class I Stabilizes T Cell Receptor-Antigen Complexes at the Cell Surface J. Biol. Chem., July 29, 2005; 280(30): 27491 - 27501. [Abstract] [Full Text] [PDF]

				R. Maile, C. A. Siler, S. E. Kerry, K. E. Midkiff, E. J. Collins, and J. A. Frelinger Peripheral "CD8 Tuning" Dynamically Modulates the Size and Responsiveness of an Antigen-Specific T Cell Pool In Vivo J. Immunol., January 15, 2005; 174(2): 619 - 627. [Abstract] [Full Text] [PDF]

				L. Wooldridge, S. L. Hutchinson, E. M. Choi, A. Lissina, E. Jones, F. Mirza, P. R. Dunbar, D. A. Price, V. Cerundolo, and A. K. Sewell Anti-CD8 Antibodies Can Inhibit or Enhance Peptide-MHC Class I (pMHCI) Multimer Binding: This Is Paralleled by Their Effects on CTL Activation and Occurs in the Absence of an Interaction between pMHCI and CD8 on the Cell Surface J. Immunol., December 15, 2003; 171(12): 6650 - 6660. [Abstract] [Full Text] [PDF]

				S. E. Kerry, J. Buslepp, L. A. Cramer, R. Maile, L. L. Hensley, A. I. Nielsen, P. Kavathas, B. J. Vilen, E. J. Collins, and J. A. Frelinger Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Affinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement J. Immunol., November 1, 2003; 171(9): 4493 - 4503. [Abstract] [Full Text] [PDF]

				K. Gamerdinger, C. Moulon, D. R. Karp, J. van Bergen, F. Koning, D. Wild, U. Pflugfelder, and H. U. Weltzien A New Type of Metal Recognition by Human T Cells: Contact Residues for Peptide-independent Bridging of T Cell Receptor and Major Histocompatibility Complex by Nickel J. Exp. Med., May 19, 2003; 197(10): 1345 - 1353. [Abstract] [Full Text] [PDF]

				G. Werlen, B. Hausmann, D. Naeher, and E. Palmer Signaling Life and Death in the Thymus: Timing Is Everything Science, March 21, 2003; 299(5614): 1859 - 1863. [Abstract] [Full Text] [PDF]

				M.-A. Doucey, L. Goffin, D. Naeher, O. Michielin, P. Baumgartner, P. Guillaume, E. Palmer, and I. F. Luescher CD3delta Establishes a Functional Link between the T Cell Receptor and CD8 J. Biol. Chem., January 24, 2003; 278(5): 3257 - 3264. [Abstract] [Full Text] [PDF]