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* Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine,
Department of Pathology,
Comprehensive Cancer Center, and
Graduate Program in Immunology, University of Michigan Health Care System, Ann Arbor, MI 48109;
¶ Department of Genomics and Pathobiology, University of Alabama, Birmingham, AL 35294; and
|| Pulmonary and Critical Care Medicine Section, Medical Service, Department of Veterans Affairs Care System, Ann Arbor, MI 48105
We previously demonstrated induction and expression of CD62E and
CD62P in the lungs of mice primed and then challenged with
intratracheal (i.t.) SRBC. The current study examined
accumulation of endogenous lymphocytes in the lungs of endothelial E-
and P-selectin-deficient (E-P-) mice after
i.t. SRBC challenge. Compared with syngeneic wild-type (wt) mice,
E-P- mice showed an
85–95% decrease in CD8+ T cells and B cells in the lungs
at both early and late time points. In contrast, CD4+ T
cell accumulation was reduced by 
60% early, but equivalent to wt
levels later. Surprisingly, many 
T cells were found in lungs and
blood of E-P- mice
but were undetectable in the lungs and blood of wt mice. Absolute
numbers of peripheral blood CD4, CD8, and B lymphocytes in
E-P- mice equaled or
exceeded the levels in wt mice, particularly after challenge.
Trafficking studies using 
T lymphoblasts confirmed that the
recruitment of circulating cells after challenge was markedly reduced
in E-P- mice.
Furthermore, Ag priming occurred normally in both the
selectin-deficient and wt mice, because primed lymphocytes from both
groups transferred Ag sensitivity into naive wt mice. Lung production
of mRNA for six CC and two CXC chemokines after challenge was
equivalent by RT-PCR analysis in wt and
E-P- mice. Therefore,
reduced lung accumulation of T cells and B cells in
E-P- mice did not
result from reduced delivery of circulating lymphocytes to the lungs,
unsuccessful Ag priming, or defective pulmonary chemokine production.
Selectin-dependent lymphocyte recruitment into the lungs following
i.t.-SRBC challenge is subset specific and time
dependent.
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