| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Division of Immunology, Aichi Cancer Center Research Institute and
Department of Hematology and Chemotherapy, Aichi Cancer Center Hospital, Nagoya, Japan;
Immunology and
Transplantation Biology, Fred Hutchinson Cancer Research Center and University of Washington, Seattle, WA 98109;
¶ Department of Biological Systems, RIKEN BioResource Center, Tsukuba Institute, RIKEN, Tsukuba, Japan;
|| Second Department of Internal Medicine, Okayama University Medical School, Okayama, Japan; and
# Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8+ T cells with these transduced CD40-B cells, both of which were derived from 10 ml peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.
This article has been cited by other articles:
|
|
 |
|
  H.-D. Hummel, G. Kuntz, S. J. Russell, T. Nakamura, A. Greiner, H. Einsele, and M. S. Topp Genetically engineered attenuated measles virus specifically infects and kills primary multiple myeloma cells J. Gen. Virol., March 1, 2009; 90(3): 693 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kondo, B. Maecker, M. R. Weihrauch, C. Wickenhauser, W. Zeng, L. M. Nadler, J. L. Schultze, and M. S. von Bergwelt-Baildon Cyclin D1-Specific Cytotoxic T Lymphocytes Are Present in the Repertoire of Cancer Patients: Implications for Cancer Immunotherapy Clin. Cancer Res., October 15, 2008; 14(20): 6574 - 6579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kawase, Y. Akatsuka, H. Torikai, S. Morishima, A. Oka, A. Tsujimura, M. Miyazaki, K. Tsujimura, K. Miyamura, S. Ogawa, et al. Alternative splicing due to an intronic SNP in HMSD generates a novel minor histocompatibility antigen Blood, August 1, 2007; 110(3): 1055 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Adamopoulou, J. Diekmann, E. Tolosa, G. Kuntz, H. Einsele, H.-G. Rammensee, and M. S. Topp Human CD4+ T Cells Displaying Viral Epitopes Elicit a Functional Virus-Specific Memory CD8+ T Cell Response J. Immunol., May 1, 2007; 178(9): 5465 - 5472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ito, A. Demachi-Okamura, R. Ohta, Y. Akatsuka, K. Nishida, K. Tsujimura, Y. Morishima, T. Takahashi, and K. Kuzushima Full-length EBNA1 mRNA-transduced dendritic cells stimulate cytotoxic T lymphocytes recognizing a novel HLA-Cw*0303- and -Cw*0304-restricted epitope on EBNA1-expressing cells J. Gen. Virol., March 1, 2007; 88(3): 770 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chung, B.-S. Kim, Y.-J. Kim, H.-J. Ko, S.-Y. Ko, D.-H. Kim, and C.-Y. Kang CD1d-Restricted T Cells License B Cells to Generate Long-Lasting Cytotoxic Antitumor Immunity In vivo. Cancer Res., July 1, 2006; 66(13): 6843 - 6850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. von Bergwelt-Baildon, A. Shimabukuro-Vornhagen, A. Popov, N. Klein-Gonzalez, F. Fiore, S. Debey, A. Draube, B. Maecker, I. Menezes, L. M. Nadler, et al. CD40-activated B cells express full lymph node homing triad and induce T-cell chemotaxis: potential as cellular adjuvants Blood, April 1, 2006; 107(7): 2786 - 2789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujiwara, J. J. Melenhorst, F. El Ouriaghli, S. Kajigaya, M. Grube, G. Sconocchia, K. Rezvani, D. A. Price, N. F. Hensel, D. C. Douek, et al. In vitro Induction of Myeloid Leukemia-Specific CD4 and CD8 T Cells by CD40 Ligand - Activated B Cells Gene Modified to Express Primary Granule Proteins Clin. Cancer Res., June 15, 2005; 11(12): 4495 - 4503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ishida, S. Iida, Y. Akatsuka, T. Ishii, M. Miyazaki, H. Komatsu, H. Inagaki, N. Okada, T. Fujita, K. Shitara, et al. The CC Chemokine Receptor 4 as a Novel Specific Molecular Target for Immunotherapy in Adult T-Cell Leukemia/Lymphoma Clin. Cancer Res., November 15, 2004; 10(22): 7529 - 7539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, C. La Rosa, S. Mekhoubad, S. F. Lacey, M. C. Villacres, S. Markel, J. Longmate, J. D. I. Ellenhorn, R. F. Siliciano, C. Buck, et al. Attenuated poxviruses generate clinically relevant frequencies of CMV-specific T cells Blood, August 1, 2004; 104(3): 847 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. von Bergwelt-Baildon, J. L. Schultze, B. Maecker, I. Menezes, L. M. Nadler, R. Lapointe, J. Thibodeau, and P. Hwu Correspondence re R. Lapointe et al., CD40-stimulated B Lymphocytes Pulsed with Tumor Antigens Are Effective Antigen-presenting Cells That Can Generate Specific T Cells. Cancer Res 2003;63:2836-43. Cancer Res., June 1, 2004; 64(11): 4055 - 4057. [Full Text] [PDF]
|
|
|
|
|
|
G. Rauser, H. Einsele, C. Sinzger, D. Wernet, G. Kuntz, M. Assenmacher, J. D. M. Campbell, and M. S. Topp Rapid generation of combined CMV-specific CD4+ and CD8+ T-cell lines for adoptive transfer into recipients of allogeneic stem cell transplants Blood, May 1, 2004; 103(9): 3565 - 3572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Coughlin, B. A. Vance, S. A. Grupp, and R. H. Vonderheide RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy Blood, March 15, 2004; 103(6): 2046 - 2054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kondo, Y. Akatsuka, K. Kuzushima, K. Tsujimura, S. Asakura, K. Tajima, Y. Kagami, Y. Kodera, M. Tanimoto, Y. Morishima, et al. Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA alleles Blood, January 15, 2004; 103(2): 630 - 638. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
