| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
* Cancer Biology and
Vascular Biology, La Jolla Institute for Molecular Medicine, San Diego, CA 92121
In leukocytes, C3a and C5a cause chemotaxis in a
Gi-dependent, pertussis toxin (PT)-sensitive fashion.
Because we found that HUVECs and immortalized human dermal
microvascular endothelial cells express small numbers of C3aRs and
C5aRs, we asked what the function of these receptors was on these
cells. Activation of the C3aR caused transient formation of actin
stress fibers, which was not PT-sensitive, but depended on rho
activation implying coupling to G
12 or
G13. Activation of the C5aR caused a delayed and
sustained cytoskeletal response, which was blocked by PT, and resulted
in cell retraction, increased paracellular permeability, and
facilitated eosinophil transmigration. C5a, but not C3a, was
chemotactic for human immortalized dermal microvascular endothelial
cells. The response to C5a was blocked by inhibitors of
phosphatidylinositol-3-kinase, src kinase, and of the epidermal growth
factor (EGF) receptor (EGFR) as well as by neutralizing Abs against the
EGFR and heparin-binding EGF-like factor. Furthermore, immune
precipitations showed that the EGFR was phosphorylated following
stimulation with C5a. The C5aR in endothelial cells thus uses a
signaling cascade–transactivation of the EGFR–that does not exist in
leukocytes, while the C3aR couples to a different G protein, presumably
G12/13.
This article has been cited by other articles:
|
|
 |
|
  M. Gorovoy, J. Han, H. Pan, E. Welch, R. Neamu, Z. Jia, D. Predescu, S. Vogel, R. D. Minshall, R. D. Ye, et al. LIM Kinase 1 Promotes Endothelial Barrier Disruption and Neutrophil Infiltration in Mouse Lungs Circ. Res., September 11, 2009; 105(6): 549 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. U. Schraufstatter, R. G. DiScipio, M. Zhao, and S. K. Khaldoyanidi C3a and C5a Are Chemotactic Factors for Human Mesenchymal Stem Cells, Which Cause Prolonged ERK1/2 Phosphorylation J. Immunol., March 15, 2009; 182(6): 3827 - 3836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Li, K. J. Anderson, Q. Peng, A. Noble, B. Lu, A. P. Kelly, N. Wang, S. H. Sacks, and W. Zhou Cyclic AMP plays a critical role in C3a-receptor-mediated regulation of dendritic cells in antigen uptake and T-cell stimulation Blood, December 15, 2008; 112(13): 5084 - 5094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Verdeguer, C. Castro, M. Kubicek, D. Pla, M. Vila-Caballer, A. Vinue, F. Civeira, M. Pocovi, J. J. Calvete, and V. Andres Complement regulation in murine and human hypercholesterolemia and role in the control of macrophage and smooth muscle cell proliferation Cardiovasc Res, November 1, 2007; 76(2): 340 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wehner, C. N. Morrell, T. Reynolds, E. R. Rodriguez, and W. M. Baldwin III Antibody and Complement in Transplant Vasculopathy Circ. Res., February 2, 2007; 100(2): 191 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Nelin, L. G. Chicoine, K. M. Reber, B. K. English, T. L. Young, and Y. Liu Cytokine-Induced Endothelial Arginase Expression Is Dependent on Epidermal Growth Factor Receptor Am. J. Respir. Cell Mol. Biol., October 1, 2005; 33(4): 394 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, J. F. Wang, B. Chandran, K. Persaud, B. Pytowski, J. Fingeroth, and J. E. Groopman Kaposi's Sarcoma-associated Herpesvirus Activation of Vascular Endothelial Growth Factor Receptor 3 Alters Endothelial Function and Enhances Infection J. Biol. Chem., July 15, 2005; 280(28): 26216 - 26224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Boos, I. L. Campbell, R. Ames, R. A. Wetsel, and S. R. Barnum Deletion of the Complement Anaphylatoxin C3a Receptor Attenuates, Whereas Ectopic Expression of C3a in the Brain Exacerbates, Experimental Autoimmune Encephalomyelitis J. Immunol., October 1, 2004; 173(7): 4708 - 4714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Braun, R. Y. Reins, T.-b. Li, T. J. Hollmann, R. Dutta, W. A. Rick, B.-B. Teng, and B. Ke Renal Expression of the C3a Receptor and Functional Responses of Primary Human Proximal Tubular Epithelial Cells J. Immunol., September 15, 2004; 173(6): 4190 - 4196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Li, H. Patel, C. A. Farrar, R. E.G. Hargreaves, S. H. Sacks, and W. Zhou Complement Activation Regulates the Capacity of Proximal Tubular Epithelial Cell to Stimulate Alloreactive T Cell Response J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2414 - 2422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Strey, M. Markiewski, D. Mastellos, R. Tudoran, L. A. Spruce, L. E. Greenbaum, and J. D. Lambris The Proinflammatory Mediators C3a and C5a Are Essential for Liver Regeneration J. Exp. Med., September 15, 2003; 198(6): 913 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. MONSINJON, P. GASQUE, P. CHAN, A. ISCHENKO, J. J. BRADY, and M. FONTAINE Regulation by complement C3a and C5a anaphylatoxins of cytokine production in human umbilical vein endothelial cells FASEB J, June 1, 2003; 17(9): 1003 - 1014. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
