| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
, Its Receptor Chain IFN- Receptor-1, and the Phosphorylation and Nuclear Translocation of STAT1
1
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611
IFN-
contains a nuclear localization sequence
that may play a role in the nuclear transport of activated STAT1
via
a complex of IFN-/IFN- receptor (IFNGR)-1/STAT1 with the
nuclear importer nucleoprotein interactor 1. In this study, we examine
the mechanism of endocytosis of IFNGR-1 and the relationship of its
nuclear translocation to that of STAT1. In untreated WISH cells,
both IFNGR-1 and IFNGR-2 were constitutively localized within
caveolae-like microdomains isolated from plasma membrane. However,
treatment of cells with IFN- resulted in rapid migration of IFNGR-1,
but not IFNGR-2, from these microdomains. Filipin pretreatment, which
specifically inhibits endocytosis from caveolae-like microdomains,
inhibited the nuclear translocation of IFN- and IFNGR-1 as well
as the tyrosine phosphorylation and nuclear translocation of STAT1,
but did not affect the binding of IFN- to these cells. In the Jurkat
T lymphocyte cell line, which does not express caveolin-1, nuclear
translocation of IFNGR-1 and STAT1 were similarly inhibited by
filipin pretreatment. Isolation of lipid microdomains from Jurkat cells
showed that both IFNGR-1 and IFNGR-2 were associated with lipid
microdomains only after stimulation with IFN-, suggesting that the
IFNGR subunits are recruited to lipid microdomains by IFN- binding
in lymphocytes (Jurkat) in contrast to their constitutive presence in
epithelial (WISH) cells. In contrast, treatments that block
clathrin-dependent endocytosis did not inhibit either activation or
nuclear translocation of STAT1 or the nuclear translocation of
IFN- or IFNGR-1. Thus, membrane lipid microdomains play an important
role in IFN--initiated endocytic events involving IFNGR-1, and the
nuclear translocation of IFN-, IFNGR-1, and
STAT1.
This article has been cited by other articles:
|
|
 |
|
  J. T. Lei and M. Martinez-Moczygemba Separate endocytic pathways regulate IL-5 receptor internalization and signaling J. Leukoc. Biol., August 1, 2008; 84(2): 499 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. I. Ahmed and H. M. Johnson IFN-{gamma} and Its Receptor Subunit IFNGR1 Are Recruited to the IFN-{gamma}-Activated Sequence Element at the Promoter Site of IFN-{gamma}-Activated Genes: Evidence of Transactivational Activity in IFNGR1 J. Immunol., July 1, 2006; 177(1): 315 - 321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Marchetti, M.-N. Monier, A. Fradagrada, K. Mitchell, F. Baychelier, P. Eid, L. Johannes, and C. Lamaze Stat-mediated Signaling Induced by Type I and Type II Interferons (IFNs) Is Differentially Controlled through Lipid Microdomain Association and Clathrin-dependent Endocytosis of IFN Receptors Mol. Biol. Cell, July 1, 2006; 17(7): 2896 - 2909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jandu, P. J. M. Ceponis, S. Kato, J. D. Riff, D. M. McKay, and P. M. Sherman Conditioned Medium from Enterohemorrhagic Escherichia coli-Infected T84 Cells Inhibits Signal Transducer and Activator of Transcription 1 Activation by Gamma Interferon Infect. Immun., March 1, 2006; 74(3): 1809 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Regis, M. Bosticardo, L. Conti, S. De Angelis, D. Boselli, B. Tomaino, P. Bernabei, M. Giovarelli, and F. Novelli Iron regulates T-lymphocyte sensitivity to the IFN-{gamma}/STAT1 signaling pathway in vitro and in vivo Blood, April 15, 2005; 105(8): 3214 - 3221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Lo, W. Xia, Y. Wei, M. Ali-Seyed, S.-F. Huang, and M.-C. Hung Novel Prognostic Value of Nuclear Epidermal Growth Factor Receptor in Breast Cancer Cancer Res., January 1, 2005; 65(1): 338 - 348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Switzer, Y.-Y. Fan, N. Wang, D. N. McMurray, and R. S. Chapkin Dietary n-3 polyunsaturated fatty acids promote activation-induced cell death in Th1-polarized murine CD4+ T-cells J. Lipid Res., August 1, 2004; 45(8): 1482 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Yang, Y. Huang, J. Jiang, and S. J. Frank Caveolar and Lipid Raft Localization of the Growth Hormone Receptor and Its Signaling Elements: IMPACT ON GROWTH HORMONE SIGNALING J. Biol. Chem., May 14, 2004; 279(20): 20898 - 20905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Cecil and M. J. Klemsz p38 activation through Toll-like receptors modulates IFN-{gamma}-induced expression of the Tap-1 gene only in macrophages J. Leukoc. Biol., March 1, 2004; 75(3): 560 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bernabei, M. Bosticardo, G. Losana, G. Regis, F. Di Paola, S. De Angelis, M. Giovarelli, and F. Novelli IGF-1 down-regulates IFN-{gamma}R2 chain surface expression and desensitizes IFN-{gamma}/STAT-1 signaling in human T lymphocytes Blood, October 15, 2003; 102(8): 2933 - 2939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gobeil Jr., S. G. Bernier, A. Vazquez-Tello, S. Brault, M. H. Beauchamp, C. Quiniou, A. M. Marrache, D. Checchin, F. Sennlaub, X. Hou, et al. Modulation of Pro-inflammatory Gene Expression by Nuclear Lysophosphatidic Acid Receptor Type-1 J. Biol. Chem., October 3, 2003; 278(40): 38875 - 38883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. I. Ahmed, M. A. Burkhart, M. G. Mujtaba, P. S. Subramaniam, and H. M. Johnson The role of IFN{gamma} nuclear localization sequence in intracellular function J. Cell Sci., August 1, 2003; 116(15): 3089 - 3098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shah, K. Patel, V. A. Fried, and P. B. Sehgal Interactions of STAT3 with Caveolin-1 and Heat Shock Protein 90 in Plasma Membrane Raft and Cytosolic Complexes. PRESERVATION OF CYTOKINE SIGNALING DURING FEVER J. Biol. Chem., November 15, 2002; 277(47): 45662 - 45669. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
