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The Th1-Specific Costimulatory Molecule, M150, Is a Posttranslational Isoform of Lysosome-Associated Membrane Protein-1¹

Durbaka V. R. Prasad^*, Vrajesh V. Parekh^*, Bimba N. Joshi^*, Pinaki P. Banerjee^*, Pradeep B. Parab^*, Samit Chattopadhyay^*, Anil Kumar and Gyan C. Mishra^2,*

^* National Centre For Cell Science, Pune, India; and School of Biotechnology, Devi Ahilya Vishwavidyalaya, Indore, India

In an earlier report, we had shown a 150-kDa protein termed as M150, isolated from the surface of activated macrophages, to possess costimulatory activity for CD4⁺ T cells. Significantly, this protein was found to specifically elicit Th1 responses. In this study, we characterize M150, which belongs to a unique subset of the lysosome-associated membrane protein-1 glycoprotein. Interestingly, the costimulatory activity of M150 depends on its posttranslational modification, which has a distinct glycosylation pattern restricted to macrophages. Furthermore, it has been demonstrated that in addition to stimulating Th1-specific responses, M150 is also capable of driving differentiation of naive CD4⁺ T cells into the Th1 subset. This altered posttranslational modification of housekeeping protein appears to represent a novel pathway by which APCs can additionally regulate T cell responses.

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