| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Institut National de la Santé et de la Recherche Médicale, Toulouse, France;
Institut Curie, Paris, France;
Division of Pulmonary and Critical Care Medicine, Veterans Affairs Medical Center, and
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland OR 97201
Infections by human CMV are controlled by cellular immune responses. Professional APC such as monocytes and macrophages can be infected in vivo and are considered as a reservoir of virus. However, CMV-specific CD4+ responses against infected APC have not been reported. To develop a model of CD4-infected APC interaction, we have transfected the U373MG astrocytoma cell line with the class II transactivator (CIITA). Confocal microscopy experiments showed that U373MG-CIITA cells expressed markers characteristic of APC. Functional assays demonstrated that infected U373MG-CIITA APC processed and presented both exogenous and endogenously neosynthesized nuclear immediate early (IE) protein 1 through the MHC class II pathway. More importantly, endogenous presentation of IE1 by infected APC lead to efficient control of CMV infection as revealed by decreased viral titer. Thus, these results describe the endogenous presentation of a nuclear viral protein by the MHC class II pathway and suggest that IE1-specific CD4+ T cells may play an important role in CMV infection by directly acting against infected APC.
This article has been cited by other articles:
|
|
 |
|
  M. Baron and J.-L. Davignon Inhibition of IFN-{gamma}-Induced STAT1 Tyrosine Phosphorylation by Human CMV Is Mediated by SHP2 J. Immunol., October 15, 2008; 181(8): 5530 - 5536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Delmas, P. Brousset, D. Clement, E. Le Roy, and J.-L. Davignon Anti-IE1 CD4+ T-cell clones kill peptide-pulsed, but not human cytomegalovirus-infected, target cells J. Gen. Virol., September 1, 2007; 88(9): 2441 - 2449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Wiertz, R. Devlin, H. L. Collins, and M. E. Ressing Herpesvirus Interference with Major Histocompatibility Complex Class II-Restricted T-Cell Activation J. Virol., May 1, 2007; 81(9): 4389 - 4396. [Full Text] [PDF]
|
|
|
|
|
|
B. Heemskerk, T. van Vreeswijk, L. A. Veltrop-Duits, C. C. Sombroek, K. Franken, R. M. Verhoosel, P. S. Hiemstra, D. van Leeuwen, M. E. Ressing, R. E. M. Toes, et al. Adenovirus-Specific CD4+ T Cell Clones Recognizing Endogenous Antigen Inhibit Viral Replication In Vitro through Cognate Interaction J. Immunol., December 15, 2006; 177(12): 8851 - 8859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-D. Park, L. Marti, J. Kurtzberg, and P. Szabolcs In vitro priming and expansion of cytomegalovirus-specific Th1 and Tc1 T cells from naive cord blood lymphocytes Blood, September 1, 2006; 108(5): 1770 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sinzger, K. Eberhardt, Y. Cavignac, C. Weinstock, T. Kessler, G. Jahn, and J.-L. Davignon Macrophage cultures are susceptible to lytic productive infection by endothelial-cell-propagated human cytomegalovirus strains and present viral IE1 protein to CD4+ T cells despite late downregulation of MHC class II molecules J. Gen. Virol., July 1, 2006; 87(7): 1853 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Delmas, L. Martin, M. Baron, J. A. Nelson, D. N. Streblow, and J.-L. Davignon Optimization of CD4+ T Lymphocyte Response to Human Cytomegalovirus Nuclear IE1 Protein through Modifications of Both Size and Cellular Localization J. Immunol., November 15, 2005; 175(10): 6812 - 6819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Allal, C. Buisson-Brenac, V. Marion, C. Claudel-Renard, T. Faraut, P. Dal Monte, D. Streblow, M. Record, and J.-L. Davignon Human Cytomegalovirus Carries a Cell-Derived Phospholipase A2 Required for Infectivity J. Virol., July 15, 2004; 78(14): 7717 - 7726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moutaftsi, P. Brennan, S. A. Spector, and Z. Tabi Impaired Lymphoid Chemokine-Mediated Migration due to a Block on the Chemokine Receptor Switch in Human Cytomegalovirus-Infected Dendritic Cells J. Virol., March 15, 2004; 78(6): 3046 - 3054. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
