| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Functional Biology, Area of Immunology, Universidad de Oviedo, and
Department of Immunology, Hospital Central de Asturias, Oviedo, Spain
After TCR cross-linking, naive
CD4+CD45RA+ T cells switch to the expression of
the CD45RO isoform and acquire effector functions. In this study we
have shown that cAMP-elevating agents added to anti-CD3- and
anti-CD28-stimulated cultures of T lymphocytes prevent acquisition
of the CD45RO+ phenotype and lead to the generation of a
new subpopulation of primed CD4+CD45RA+
effector cells (cAMP-primed CD45RA). These cells displayed a low
apoptotic index, as the presence of dibutyryl cAMP (dbcAMP)-rescued
cells from CD3/CD28 induced apoptosis. Inhibition of CD45 splicing by
dbcAMP was not reverted by addition of exogenous IL-2. cAMP-primed
CD45RA cells had a phenotype characteristic of memory/effector T
lymphocytes, as they showed an up-regulated expression of CD2, CD44,
and CD11a molecules, while the levels of CD62L Ag were down-regulated.
These cells also expressed the activation markers CD30, CD71, and HLA
class II Ags at an even higher level than CD3/CD28-stimulated cells in
the absence of dbcAMP. In agreement with this finding, cAMP-primed
CD45RA cells were very efficient in triggering allogenic responses in a
MLR. In addition, cAMP-primed CD45RA cells produce considerable amounts
of the Th2 cytokines, IL-4, IL-10, and IL-13, whereas the production of
IFN-
and TNF-
was nearly undetectable. The elevated production of
IL-13 by neonatal and adult cAMP-primed CD45RA cells was specially
noticeable. The cAMP-dependent inhibition of CD45 splicing was not
caused by the production of immunosuppressor cytokines. These results
suggest that within the pool of CD4+CD45RA+
cells there is a subpopulation of effector lymphocytes generated by
activation in the presence of cAMP-elevating
agents.
This article has been cited by other articles:
|
|
 |
|
  K. Nakano, T. Higashi, R. Takagi, K. Hashimoto, Y. Tanaka, and S. Matsushita Dopamine released by dendritic cells polarizes Th2 differentiation Int. Immunol., June 1, 2009; 21(6): 645 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Miyazaki, K Iwabuchi, S Kikuchi, T Fukazawa, M Niino, M Hirotani, H Sasaki, and K Onoe Expansion of CD4+CD28- T cells producing high levels of interferon-{gamma} in peripheral blood of patients with multiple sclerosis Multiple Sclerosis, September 1, 2008; 14(8): 1044 - 1055. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Naji, S. Le Rond, A. Durrbach, I. Krawice-Radanne, C. Creput, M. Daouya, J. Caumartin, J. LeMaoult, E. D. Carosella, and N. Rouas-Freiss CD3+CD4low and CD3+CD8low are induced by HLA-G: novel human peripheral blood suppressor T-cell subsets involved in transplant acceptance Blood, December 1, 2007; 110(12): 3936 - 3948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Yang, K. W. McIntyre, R. M. Townsend, H. H. Shen, W. J. Pitts, J. H. Dodd, S. G. Nadler, M. McKinnon, and A. J. Watson Phosphodiesterase 7A-Deficient Mice Have Functional T Cells J. Immunol., December 15, 2003; 171(12): 6414 - 6420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Masci, M. Galgani, S. Cassano, S. De Simone, A. Gallo, V. De Rosa, S. Zappacosta, and L. Racioppi HIV-1 gp120 induces anergy in naive T lymphocytes through CD4-independent protein kinase-A-mediated signaling J. Leukoc. Biol., December 1, 2003; 74(6): 1117 - 1124. [Abstract] [Full Text]
|
|
|
|
|
|
D. Wingett and C. P. Nielson Divergence in NK cell and cyclic AMP regulation of T cell CD40L expression in asthmatic subjects J. Leukoc. Biol., October 1, 2003; 74(4): 531 - 541. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
