HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arakawa, H. Articles by Yamagishi, H. Search for Related Content PubMed PubMed Citation Articles by Arakawa, H. Articles by Yamagishi, H.

Effect of Environmental Antigens on the Ig Diversification and the Selection of Productive V-J Joints in the Bursa¹

Hiroshi Arakawa^2,*,, Kei-ichi Kuma^*, Masahiro Yasuda, Shigeo Ekino, Akira Shimizu and Hideo Yamagishi^3,*,

^* Department of Biophysics, Graduate School of Science, Center for Molecular Biology and Genetics, Kyoto University, and Health Research Foundation, Kyoto, Japan; and Department of Anatomy, Kumamoto University Medical School, Kumamoto, Japan

In chickens, a single set of unique functional segments of both Ig H and L chain genes is rearranged during early embryogenesis to generate a pool of B cell progenitors that will be diversified in the bursa by gene conversion, forming the preimmune repertoire. After hatching, bursal cells are exposed to environmental Ags in the bursal lumen. We prepared B cells from each single bursal follicle and used PCR-directed Ig L chain gene analysis to study the differentiation of B cells and the effect of antigenic stimulation from the bursal lumen on the neonatal chicken B cell repertoire formation. Selective amplification of B cell clones with a productive V-J joint was observed during the late embryonic stage, possibly by the interaction with ligands expressed on the bursal stroma and further accelerated in the neonatal chicken. Administration of the artificial Ags into the bursal lumen before the isolation of bursa by bursal duct ligation in the embryo caused a significant increase in lymphocytes with a productive V-J joint in the neonatal chicken bursa compared with the isolated bursa. Intra- and interclonal diversity of a complementarity-determining region measured by an evolutionary distance increased during bursal development. Clonal diversification did not require stimulation by artificial Ags from the bursal lumen. Thus, the preimmune repertoire in the bursa is generated by gene conversion during Ag-independent B cell proliferation, and antigenic stimulation from the bursal epithelium to bursal B cells plays roles in the selection of clones with a productive V-J joint.

This article has been cited by other articles:

				J. J. Dibner, J. D. Richards, and C. D. Knight Microbial Imprinting in Gut Development and Health J. Appl. Poult. Res., January 1, 2008; 17(1): 174 - 188. [Abstract] [Full Text] [PDF]

				C. Hansell, X. W. Zhu, H. Brooks, M. Sheppard, S. Withanage, D. Maskell, and I. McConnell Unique Features and Distribution of the Chicken CD83+ Cell J. Immunol., October 15, 2007; 179(8): 5117 - 5125. [Abstract] [Full Text] [PDF]

				N. D'Avirro, D. Truong, B. Xu, and E. Selsing Sequence Transfers between Variable Regions in a Mouse Antibody Transgene Can Occur by Gene Conversion J. Immunol., December 15, 2005; 175(12): 8133 - 8137. [Abstract] [Full Text] [PDF]

				R. Mehr, H. Edelman, D. Sehgal, and R. Mage Analysis of Mutational Lineage Trees from Sites of Primary and Secondary Ig Gene Diversification in Rabbits and Chickens J. Immunol., April 15, 2004; 172(8): 4790 - 4796. [Abstract] [Full Text] [PDF]

				P. J. Jasper, S.-K. Zhai, S. L. Kalis, M. Kingzette, and K. L. Knight B Lymphocyte Development in Rabbit: Progenitor B Cells and Waning of B Lymphopoiesis J. Immunol., December 15, 2003; 171(12): 6372 - 6380. [Abstract] [Full Text] [PDF]