| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Activation and NF-B-Mediated Gene Expression Through Suppression of IB
Kinase and p65 Phosphorylation1
Cytokine Research Laboratory, Department of Bioimmunotherapy, University of Texas, M. D. Anderson Cancer Center, Houston, TX 77030
Piceatannol is an anti-inflammatory, immunomodulatory, and
anti-proliferative stilbene that has been shown to interfere with
the cytokine signaling pathway. Previously, we have shown that
resveratrol suppresses the activation of the nuclear transcription
factor NF-
B. Piceatannol, previously reported as a selective
inhibitor of protein tyrosine kinase Syk, is structurally homologous to
resveratrol. Whether piceatannol can also suppress NF-B activation
was investigated. The treatment of human myeloid cells with piceatannol
suppressed TNF-induced DNA binding activity of NF-B. In contrast,
stilbene or rhaponticin (another analog of piceatannol) had no effect,
suggesting the critical role of hydroxyl groups. The effect of
piceatannol was not restricted to myeloid cells, as TNF-induced NF-B
activation was also suppressed in lymphocyte and epithelial cells.
Piceatannol also inhibited NF-B activated by
H2O2, PMA, LPS, okadaic acid, and ceramide.
Piceatannol abrogated the expression of TNF-induced NF-B-dependent
reporter gene and of matrix metalloprotease-9, cyclooxygenase-2, and
cyclin D1. When examined for the mechanism, we found that piceatannol
inhibited TNF-induced IB
phosphorylation, p65 phosphorylation,
p65 nuclear translocation, and IB kinase activation, but had no
significant effect on IB degradation. Piceatannol inhibited
NF-B in cells with deleted Syk, indicating the lack of involvement
of this kinase. Overall, our results clearly demonstrate that hydroxyl
groups of stilbenes are critical and that piceatannol, a
tetrahydroxystilbene, suppresses NF-B activation induced by various
inflammatory agents through inhibition of IB kinase and p65
phosphorylation.
This article has been cited by other articles:
|
|
 |
|
  A. C. Klimowicz, S. A. Bisson, K. Hans, E. M. Long, H. C. Hansen, and S. M. Robbins The phytochemical piceatannol induces the loss of CBL and CBL-associated proteins Mol. Cancer Ther., March 1, 2009; 8(3): 602 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Bijli, F. Fazal, M. Minhajuddin, and A. Rahman Activation of Syk by Protein Kinase C-{delta} Regulates Thrombin-induced Intercellular Adhesion Molecule-1 Expression in Endothelial Cells via Tyrosine Phosphorylation of RelA/p65 J. Biol. Chem., May 23, 2008; 283(21): 14674 - 14684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Waldeck-Weiermair, C. Zoratti, K. Osibow, N. Balenga, E. Goessnitzer, M. Waldhoer, R. Malli, and W. F. Graier Integrin clustering enables anandamide-induced Ca2+ signaling in endothelial cells via GPR55 by protection against CB1-receptor-triggered repression J. Cell Sci., May 15, 2008; 121(10): 1704 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Ungvari, Z. Orosz, A. Rivera, N. Labinskyy, Z. Xiangmin, S. Olson, A. Podlutsky, and A. Csiszar Resveratrol increases vascular oxidative stress resistance Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2417 - H2424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gilbert, C. Barat, R. Cantin, and M. J. Tremblay Involvement of Src and Syk Tyrosine Kinases in HIV-1 Transfer from Dendritic Cells to CD4+ T Lymphocytes J. Immunol., March 1, 2007; 178(5): 2862 - 2871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Ahn, G. Sethi, K. Krishnan, and B. B. Aggarwal {gamma}-Tocotrienol Inhibits Nuclear Factor-{kappa}B Signaling Pathway through Inhibition of Receptor-interacting Protein and TAK1 Leading to Suppression of Antiapoptotic Gene Products and Potentiation of Apoptosis J. Biol. Chem., January 5, 2007; 282(1): 809 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. David, A. Saez-Cirion, P. Versmisse, O. Malbec, B. Iannascoli, F. Herschke, M. Lucas, F. Barre-Sinoussi, J.-F. Mouscadet, M. Daeron, et al. The Engagement of Activating Fc{gamma}Rs Inhibits Primate Lentivirus Replication in Human Macrophages J. Immunol., November 1, 2006; 177(9): 6291 - 6300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Csiszar, K. Smith, N. Labinskyy, Z. Orosz, A. Rivera, and Z. Ungvari Resveratrol attenuates TNF-{alpha}-induced activation of coronary arterial endothelial cells: role of NF-{kappa}B inhibition. Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1694 - H1699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pilling, N. M. Tucker, and R. H. Gomer Aggregated IgG inhibits the differentiation of human fibrocytes J. Leukoc. Biol., June 1, 2006; 79(6): 1242 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ray, M. Kuwahara, Y. Takada, K. Maruyama, T. Kawaguchi, H. Tsubone, H. Ishikawa, and K. Matsuo c-Fos suppresses systemic inflammatory response to endotoxin Int. Immunol., May 1, 2006; 18(5): 671 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takada, H. Ichikawa, V. Badmaev, and B. B. Aggarwal Acetyl-11-Keto-beta-Boswellic Acid Potentiates Apoptosis, Inhibits Invasion, and Abolishes Osteoclastogenesis by Suppressing NF-{kappa}B and NF-{kappa}B-Regulated Gene Expression. J. Immunol., March 1, 2006; 176(5): 3127 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Martin Targeting Apoptosis with Dietary Bioactive Agents Experimental Biology and Medicine, February 1, 2006; 231(2): 117 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sekine, T. Yumioka, T. Yamamoto, R. Muromoto, S. Imoto, K. Sugiyma, K. Oritani, K. Shimoda, M. Minoguchi, S. Akira, et al. Modulation of TLR4 Signaling by a Novel Adaptor Protein Signal-Transducing Adaptor Protein-2 in Macrophages J. Immunol., January 1, 2006; 176(1): 380 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Youn, J. Y. Lee, K. A. Fitzgerald, H. A. Young, S. Akira, and D. H. Hwang Specific Inhibition of MyD88-Independent Signaling Pathways of TLR3 and TLR4 by Resveratrol: Molecular Targets Are TBK1 and RIP1 in TRIF Complex J. Immunol., September 1, 2005; 175(5): 3339 - 3346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, C. Lin, and Z.-R. Liu Phosphorylations of DEAD Box p68 RNA Helicase Are Associated with Cancer Development and Cell Proliferation Mol. Cancer Res., June 1, 2005; 3(6): 355 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ulanova, L. Puttagunta, M. Marcet-Palacios, M. Duszyk, U. Steinhoff, F. Duta, M.-K. Kim, Z. K. Indik, A. D. Schreiber, and A. D. Befus Syk tyrosine kinase participates in {beta}1-integrin signaling and inflammatory responses in airway epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L497 - L507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takada and B. B. Aggarwal Evidence that genetic deletion of the TNF receptor p60 or p80 in macrophages modulates RANKL-induced signaling Blood, December 15, 2004; 104(13): 4113 - 4121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Storz, H. Doppler, and A. Toker Activation Loop Phosphorylation Controls Protein Kinase D-Dependent Activation of Nuclear Factor {kappa}B Mol. Pharmacol., October 1, 2004; 66(4): 870 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takada and B. B. Aggarwal TNF Activates Syk Protein Tyrosine Kinase Leading to TNF-Induced MAPK Activation, NF-{kappa}B Activation, and Apoptosis J. Immunol., July 15, 2004; 173(2): 1066 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takada, F. R. Khuri, and B. B. Aggarwal Protein Farnesyltransferase Inhibitor (SCH 66336) Abolishes NF-{kappa}B Activation Induced by Various Carcinogens and Inflammatory Stimuli Leading to Suppression of NF-{kappa}B-regulated Gene Expression and Up-regulation of Apoptosis J. Biol. Chem., June 18, 2004; 279(25): 26287 - 26299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takada, S. Singh, and B. B. Aggarwal Identification of a p65 Peptide That Selectively Inhibits NF-{kappa} B Activation Induced by Various Inflammatory Stimuli and Its Role in Down-regulation of NF-{kappa}B-mediated Gene Expression and Up-regulation of Apoptosis J. Biol. Chem., April 9, 2004; 279(15): 15096 - 15104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. PERVAIZ Resveratrol: from grapevines to mammalian biology FASEB J, November 1, 2003; 17(14): 1975 - 1985. [Full Text] [PDF]
|
|
|
|
|
|
Y. Takada and B. B. Aggarwal Betulinic Acid Suppresses Carcinogen-Induced NF-{kappa}B Activation Through Inhibition of I{kappa}B{alpha} Kinase and p65 Phosphorylation: Abrogation of Cyclooxygenase-2 and Matrix Metalloprotease-9 J. Immunol., September 15, 2003; 171(6): 3278 - 3286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takada and B. B. Aggarwal Genetic Deletion of the Tumor Necrosis Factor Receptor p60 or p80 Sensitizes Macrophages to Lipopolysaccharide-induced Nuclear Factor-{kappa}B, Mitogen-activated Protein Kinases, and Apoptosis J. Biol. Chem., June 20, 2003; 278(26): 23390 - 23397. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
