| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
* Pharmacology and Therapeutics and
Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada;
Departments of Pediatrics, Ophthalmology, and Pharmacology, Research Center of Sainte-Justine Hospital, Montreal, Quebec, Canada;
Departments of Pediatrics and Cell Biology, Université de Sherbrooke, Sherbrooke, Quebec, Canada;
¶ Institut de Cardiologie de Montréal, Montreal, Quebec, Canada; and
|| Research Center of Maisonneuve-Rosemont Hospital, Montreal, Quebec, Canada.
It has been postulated that intracellular binding sites for
platelet-activating factor (PAF) contribute to proinflammatory
responses to PAF. Isolated nuclei from porcine cerebral microvascular
endothelial cells (PCECs) produced PAF-molecular species in response to
H2O2. Using FACS analysis, we demonstrated the
expression of PAF receptors on cell and nuclear surfaces of PCECs.
Confocal microscopy studies performed on PCECs, Chinese hamster ovary
cells stably overexpressing PAF receptors, and isolated nuclei from
PCECs also showed a robust nuclear distribution of PAF receptors.
Presence of PAF receptors at the cell nucleus was further revealed in
brain endothelial cells by radioligand binding experiments,
immunoblotting, and in situ in brain by immunoelectron microscopy.
Stimulation of nuclei with methylcarbamate-PAF evoked a decrease in
cAMP production and a pertussis toxin-sensitive rise in nuclear
calcium, unlike observations in plasma membrane, which exhibited a
pertussis toxin-insensitive elevation in inositol phosphates. Moreover,
on isolated nuclei methylcarbamate-PAF evoked the expression of
proinflammatory genes inducible nitric oxide synthase and
cyclooxygenase-2 (COX-2) and was associated with augmented
extracellular signal-regulated kinase 1/2 phosphorylation and NF-
B
binding to the DNA consensus sequence. COX-2 expression was prevented
by mitogen-activated protein kinase kinase/extracellular
signal-regulated kinase 1/2 and NF-B inhibitors. This study
describes for the first time the nucleus as a putative organelle
capable of generating PAF and expresses its receptor, which upon
stimulation induces the expression of the proinflammatory gene
COX-2.
This article has been cited by other articles:
|
|
 |
|
  I. U. Schraufstatter, R. G. DiScipio, M. Zhao, and S. K. Khaldoyanidi C3a and C5a Are Chemotactic Factors for Human Mesenchymal Stem Cells, Which Cause Prolonged ERK1/2 Phosphorylation J. Immunol., March 15, 2009; 182(6): 3827 - 3836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Mir and G. C. Le Breton A Novel Nuclear Signaling Pathway for Thromboxane A2 Receptors in Oligodendrocytes: Evidence for Signaling Compartmentalization during Differentiation Mol. Cell. Biol., October 15, 2008; 28(20): 6329 - 6341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Quiniou, P. Sapieha, I. Lahaie, X. Hou, S. Brault, M. Beauchamp, M. Leduc, L. Rihakova, J.-S. Joyal, S. Nadeau, et al. Development of a Novel Noncompetitive Antagonist of IL-1 Receptor J. Immunol., May 15, 2008; 180(10): 6977 - 6987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fillon, K. Soulis, S. Rajasekaran, H. Benedict-Hamilton, J. N. Radin, C. J. Orihuela, K. C. El Kasmi, G. Murti, D. Kaushal, M. W. Gaber, et al. Platelet-Activating Factor Receptor and Innate Immunity: Uptake of Gram-Positive Bacterial Cell Wall into Host Cells and Cell-Specific Pathophysiology J. Immunol., November 1, 2006; 177(9): 6182 - 6191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Boivin, C. Lavoie, G. Vaniotis, A. Baragli, L.-R. Villeneuve, N. Ethier, P. Trieu, B. G. Allen, and T. E. Hebert Functional {beta}-adrenergic receptor signalling on nuclear membranes in adult rat and mouse ventricular cardiomyocytes Cardiovasc Res, July 1, 2006; 71(1): 69 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gobeil Jr., T. Zhu, S. Brault, A. Geha, A. Vazquez-Tello, A. Fortier, D. Barbaz, D. Checchin, X. Hou, M. Nader, et al. Nitric Oxide Signaling via Nuclearized Endothelial Nitric-oxide Synthase Modulates Expression of the Immediate Early Genes iNOS and mPGES-1 J. Biol. Chem., June 9, 2006; 281(23): 16058 - 16067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. He and H. E. P. Bazan Synergistic Effect of Platelet-Activating Factor and Tumor Necrosis Factor-{alpha} on Corneal Myofibroblast Apoptosis. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 883 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. O. Melnikova, A. A. Mourad-Zeidan, D. C. Lev, and M. Bar-Eli Platelet-activating Factor Mediates MMP-2 Expression and Activation via Phosphorylation of cAMP-response Element-binding Protein and Contributes to Melanoma Metastasis J. Biol. Chem., February 3, 2006; 281(5): 2911 - 2922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vazquez-Tello, L. Fan, X. Hou, J.-S. Joyal, J. A. Mancini, C. Quiniou, R. I. Clyman, F. Gobeil Jr., D. R. Varma, and S. Chemtob Intracellular-specific colocalization of prostaglandin E2 synthases and cyclooxygenases in the brain Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1155 - R1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rollin, C. Lemieux, R. Maliba, J. Favier, L. R. Villeneuve, B. G. Allen, S. Soker, N. G. Bazan, Y. Merhi, and M. G. Sirois VEGF-mediated endothelial P-selectin translocation: role of VEGF receptors and endogenous PAF synthesis Blood, May 15, 2004; 103(10): 3789 - 3797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. WARNER and J. A. MITCHELL Cyclooxygenases: new forms, new inhibitors, and lessons from the clinic FASEB J, May 1, 2004; 18(7): 790 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bouayad, J.-C. Fouron, X. Hou, M. Beauchamp, C. Quiniou, D. Abran, K. Peri, R. I. Clyman, D. R. Varma, and S. Chemtob Developmental regulation of prostaglandin E2 synthase in porcine ductus arteriosus Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2004; 286(5): R903 - R909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. G. Bazan Synaptic lipid signaling: significance of polyunsaturated fatty acids and platelet-activating factor J. Lipid Res., December 1, 2003; 44(12): 2221 - 2233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Sennlaub, F. Valamanesh, A. Vazquez-Tello, A.M. El-Asrar, D. Checchin, S. Brault, F. Gobeil, M.H. Beauchamp, B. Mwaikambo, Y. Courtois, et al. Cyclooxygenase-2 in Human and Experimental Ischemic Proliferative Retinopathy Circulation, July 15, 2003; 108(2): 198 - 204. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
