HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by García-Ramallo, E. Articles by Godessart, N. Search for Related Content PubMed PubMed Citation Articles by García-Ramallo, E. Articles by Godessart, N.

Resident Cell Chemokine Expression Serves as the Major Mechanism for Leukocyte Recruitment During Local Inflammation

Eva García-Ramallo^*, Teresa Marques^*, Neus Prats^*, Jordi Beleta^*, Steven L. Kunkel and Nuria Godessart^1,*

^* Department of Pharmacology, Almirall Prodesfarma Research Center, Barcelona, Spain; and Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109

The mechanistic relationships between initiating stimulus, cellular source and sequence of chemokine expression, and leukocyte recruitment during inflammation are not clear. To study these relationships in an acute inflammatory process, we challenged a murine air pouch with carrageenan. A time-dependent increase in TNF-, monocyte chemottractant protein-1 (MCP-1), macrophage-inflammatory protein-1 (MIP-1), RANTES, KC, and MIP-2 was found in the exudates preceding cell recruitment, but displaying different kinetic profiles. Air pouches generated for 2, 6, or 9 days before initiating inflammation demonstrated a proportional increase in the number of cells lining the cavities. Two hours after carrageenan stimulation, the synthesis of TNF- and all chemokines but RANTES increased in proportion to the lining cellularity, although no differences in infiltrating leukocytes were found, suggesting that the early source of these mediators is resident cells. To assess the contribution of neutrophils to chemokine synthesis at later time points, we used neutropenic animals. Neutrophil depletion caused a decrease in TNF- (51%), KC (37%), MIP-1 (30%), and RANTES (57%) levels and a 2-fold increase in monocytes 4 h after challenge. No effect on MIP-2 and MCP-1 levels was observed. The selective blockade of CXCR2 or CCR1 inhibited neutrophil recruitment by 74% and 54%, respectively, without a significant inhibition of monocytes. A differential effect on TNF- and MCP-1 levels was observed after these treatments, indicating that the two receptors did not subserve a mere redundant chemotactic role. Overall, our results suggest that chemokines synthesized by resident cells play an important role in the evolution of the inflammatory response.

This article has been cited by other articles:

				T. Secher, V. Vasseur, D. M. Poisson, J. A. Mitchell, F. Q. Cunha, J. C. Alves-Filho, and B. Ryffel Crucial Role of TNF Receptors 1 and 2 in the Control of Polymicrobial Sepsis J. Immunol., June 15, 2009; 182(12): 7855 - 7864. [Abstract] [Full Text] [PDF]

				M. Chopra, J. S. Reuben, and A. C. Sharma Acute Lung Injury:Apoptosis and Signaling Mechanisms Experimental Biology and Medicine, April 1, 2009; 234(4): 361 - 371. [Abstract] [Full Text] [PDF]

				J. C. J. Ray, J. L. Flynn, and D. E. Kirschner Synergy between Individual TNF-Dependent Functions Determines Granuloma Performance for Controlling Mycobacterium tuberculosis Infection J. Immunol., March 15, 2009; 182(6): 3706 - 3717. [Abstract] [Full Text] [PDF]

				J. Hoover-Plow, E. Hart, Y. Gong, A. Shchurin, and T. Schneeman A Physiological Function for Apolipoprotein(a): A Natural Regulator of the Inflammatory Response Experimental Biology and Medicine, January 1, 2009; 234(1): 28 - 34. [Abstract] [Full Text] [PDF]

				N. Dumont, P. Bouchard, and J. Frenette Neutrophil-induced skeletal muscle damage: a calculated and controlled response following hindlimb unloading and reloading Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2008; 295(6): R1831 - R1838. [Abstract] [Full Text] [PDF]

				M. von Kockritz-Blickwede, M. Rohde, S. Oehmcke, L. S. Miller, A. L. Cheung, H. Herwald, S. Foster, and E. Medina Immunological Mechanisms Underlying the Genetic Predisposition to Severe Staphylococcus aureus Infection in the Mouse Model Am. J. Pathol., December 1, 2008; 173(6): 1657 - 1668. [Abstract] [Full Text] [PDF]

				H. Wen, M. A. Schaller, Y. Dou, C. M. Hogaboam, and S. L. Kunkel Dendritic cells at the interface of innate and acquired immunity: the role for epigenetic changes J. Leukoc. Biol., March 1, 2008; 83(3): 439 - 446. [Abstract] [Full Text] [PDF]

				A. M. O'Rourke, E. Y. Wang, A. Miller, E. M. Podar, K. Scheyhing, L. Huang, C. Kessler, H. Gao, H.-T. Ton-Nu, M. T. MacDonald, et al. Anti-Inflammatory Effects of LJP 1586 [Z-3-Fluoro-2-(4-methoxybenzyl)allylamine Hydrochloride], an Amine-Based Inhibitor of Semicarbazide-Sensitive Amine Oxidase Activity J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 867 - 875. [Abstract] [Full Text] [PDF]

				B. Rabe, A. Chalaris, U. May, G. H. Waetzig, D. Seegert, A. S. Williams, S. A. Jones, S. Rose-John, and J. Scheller Transgenic blockade of interleukin 6 transsignaling abrogates inflammation Blood, February 1, 2008; 111(3): 1021 - 1028. [Abstract] [Full Text] [PDF]

				A. M. Wengner, S. C. Pitchford, R. C. Furze, and S. M. Rankin The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation Blood, January 1, 2008; 111(1): 42 - 49. [Abstract] [Full Text] [PDF]

				M. Pedrazzi, M. Patrone, M. Passalacqua, E. Ranzato, D. Colamassaro, B. Sparatore, S. Pontremoli, and E. Melloni Selective Proinflammatory Activation of Astrocytes by High-Mobility Group Box 1 Protein Signaling J. Immunol., December 15, 2007; 179(12): 8525 - 8532. [Abstract] [Full Text] [PDF]

				Y. N. A. Nabah, M. Losada, R. Estelles, T. Mateo, C. Company, L. Piqueras, C. Lopez-Gines, H. Sarau, J. Cortijo, E. J. Morcillo, et al. CXCR2 Blockade Impairs Angiotensin II Induced CC Chemokine Synthesis and Mononuclear Leukocyte Infiltration Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2370 - 2376. [Abstract] [Full Text] [PDF]

				A. Chalaris, B. Rabe, K. Paliga, H. Lange, T. Laskay, C. A. Fielding, S. A. Jones, S. Rose-John, and J. Scheller Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils Blood, September 15, 2007; 110(6): 1748 - 1755. [Abstract] [Full Text] [PDF]

				M. B. Rookmaaker, M. C. Verhaar, H. C. de Boer, R. Goldschmeding, J. A. Joles, H. A. Koomans, H.-J. Grone, and T. J. Rabelink Met-RANTES reduces endothelial progenitor cell homing to activated (glomerular) endothelium in vitro and in vivo Am J Physiol Renal Physiol, August 1, 2007; 293(2): F624 - F630. [Abstract] [Full Text] [PDF]

				M. Frink, A. Lu, B. M. Thobe, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, S. L. Kunkel, and I. H. Chaudry Monocyte chemoattractant protein-1 influences trauma-hemorrhage-induced distal organ damage via regulation of keratinocyte-derived chemokine production Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1110 - R1116. [Abstract] [Full Text] [PDF]

				T. Laragione, N. C. Yarlett, M. Brenner, A. Mello, B. Sherry, E. J. Miller, C. N. Metz, and P. S. Gulko The Arthritis Severity Quantitative Trait Loci Cia4 and Cia6 Regulate Neutrophil Migration into Inflammatory Sites and Levels of TNF-{alpha} and Nitric Oxide J. Immunol., February 15, 2007; 178(4): 2344 - 2351. [Abstract] [Full Text] [PDF]

				A. V. Chintakuntlawar, R. Astley, and J. Chodosh Adenovirus Type 37 Keratitis in the C57BL/6J Mouse Invest. Ophthalmol. Vis. Sci., February 1, 2007; 48(2): 781 - 788. [Abstract] [Full Text] [PDF]

				M. Frink, B. M. Thobe, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry 17beta-Estradiol inhibits keratinocyte-derived chemokine production following trauma-hemorrhage Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L585 - L591. [Abstract] [Full Text] [PDF]

				C. R. Bonnett, E. J. Cornish, A. G. Harmsen, and J. B. Burritt Early Neutrophil Recruitment and Aggregation in the Murine Lung Inhibit Germination of Aspergillus fumigatus Conidia Infect. Immun., December 1, 2006; 74(12): 6528 - 6539. [Abstract] [Full Text] [PDF]

				S. Y. Kim, A. D. Nguyen, J.-L. Gao, P. M. Murphy, B. C. Mansfield, and J. Y. Chou Bone Marrow-derived Cells Require a Functional Glucose 6-Phosphate Transporter for Normal Myeloid Functions J. Biol. Chem., September 29, 2006; 281(39): 28794 - 28801. [Abstract] [Full Text] [PDF]

				P. J. Lafontant, A. R. Burns, E. Donnachie, S. B. Haudek, C. W. Smith, and M. L. Entman Oncostatin M differentially regulates CXC chemokines in mouse cardiac fibroblasts Am J Physiol Cell Physiol, July 1, 2006; 291(1): C18 - C26. [Abstract] [Full Text] [PDF]

				C. A. Reichel, A. Khandoga, H.-J. Anders, D. Schlondorff, B. Luckow, and F. Krombach Chemokine receptors Ccr1, Ccr2, and Ccr5 mediate neutrophil migration to postischemic tissue J. Leukoc. Biol., January 1, 2006; 79(1): 114 - 122. [Abstract] [Full Text] [PDF]

				J. Lomas-Neira, C.-S. Chung, M. Perl, S. Gregory, W. Biffl, and A. Ayala Role of alveolar macrophage and migrating neutrophils in hemorrhage-induced priming for ALI subsequent to septic challenge Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L51 - L58. [Abstract] [Full Text] [PDF]

				C. R. Teixeira, M. J. Teixeira, R. B. B. Gomes, C. S. Santos, B. B. Andrade, I. Raffaele-Netto, J. S. Silva, A. Guglielmotti, J. C. Miranda, A. Barral, et al. Saliva from Lutzomyia longipalpis Induces CC Chemokine Ligand 2/Monocyte Chemoattractant Protein-1 Expression and Macrophage Recruitment J. Immunol., December 15, 2005; 175(12): 8346 - 8353. [Abstract] [Full Text] [PDF]

				T. Iyoda, K. Nagata, M. Akashi, and Y. Kobayashi Neutrophils Accelerate Macrophage-Mediated Digestion of Apoptotic Cells In Vivo as Well as In Vitro J. Immunol., September 15, 2005; 175(6): 3475 - 3483. [Abstract] [Full Text] [PDF]

				H. Echchannaoui, K. Frei, M. Letiembre, R. M. Strieter, Y. Adachi, and R. Landmann CD14 deficiency leads to increased MIP-2 production, CXCR2 expression, neutrophil transmigration, and early death in pneumococcal infection J. Leukoc. Biol., September 1, 2005; 78(3): 705 - 715. [Abstract] [Full Text] [PDF]

				J. L. Lomas-Neira, C.-S. Chung, D. E. Wesche, M. Perl, and A. Ayala In vivo gene silencing (with siRNA) of pulmonary expression of MIP-2 versus KC results in divergent effects on hemorrhage-induced, neutrophil-mediated septic acute lung injury J. Leukoc. Biol., June 1, 2005; 77(6): 846 - 853. [Abstract] [Full Text] [PDF]

				R. W. Saeed, S. Varma, T. Peng-Nemeroff, B. Sherry, D. Balakhaneh, J. Huston, K. J. Tracey, Y. Al-Abed, and C. N. Metz Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation J. Exp. Med., April 4, 2005; 201(7): 1113 - 1123. [Abstract] [Full Text] [PDF]

				M. H. Braff, M. A. Hawkins, A. D. Nardo, B. Lopez-Garcia, M. D. Howell, C. Wong, K. Lin, J. E. Streib, R. Dorschner, D. Y. M. Leung, et al. Structure-Function Relationships among Human Cathelicidin Peptides: Dissociation of Antimicrobial Properties from Host Immunostimulatory Activities J. Immunol., April 1, 2005; 174(7): 4271 - 4278. [Abstract] [Full Text] [PDF]

				J.-S. Cadieux, P. Leclerc, M. St-Onge, A.-A. Dussault, C. Laflamme, S. Picard, C. Ledent, P. Borgeat, and M. Pouliot Potentiation of neutrophil cyclooxygenase-2 by adenosine: an early anti-inflammatory signal J. Cell Sci., April 1, 2005; 118(7): 1437 - 1447. [Abstract] [Full Text] [PDF]

				R. W. Saeed, S. Varma, T. Peng, K. J. Tracey, B. Sherry, and C. N. Metz Ethanol Blocks Leukocyte Recruitment and Endothelial Cell Activation In Vivo and In Vitro J. Immunol., November 15, 2004; 173(10): 6376 - 6383. [Abstract] [Full Text] [PDF]

				J. L. Lomas-Neira, C.-S. Chung, P. S. Grutkoski, E. J. Miller, and A. Ayala CXCR2 inhibition suppresses hemorrhage-induced priming for acute lung injury in mice J. Leukoc. Biol., July 1, 2004; 76(1): 58 - 64. [Abstract] [Full Text] [PDF]

				G.-H. Fan, L. A. Lapierre, J. R. Goldenring, J. Sai, and A. Richmond Rab11-Family Interacting Protein 2 and Myosin Vb Are Required for CXCR2 Recycling and Receptor-mediated Chemotaxis Mol. Biol. Cell, May 1, 2004; 15(5): 2456 - 2469. [Abstract] [Full Text] [PDF]

				K. R. Taylor, J. M. Trowbridge, J. A. Rudisill, C. C. Termeer, J. C. Simon, and R. L. Gallo Hyaluronan Fragments Stimulate Endothelial Recognition of Injury through TLR4 J. Biol. Chem., April 23, 2004; 279(17): 17079 - 17084. [Abstract] [Full Text] [PDF]

				C. Cataisson, E. Joseloff, R. Murillas, A. Wang, C. Atwell, S. Torgerson, M. Gerdes, J. Subleski, J.-L. Gao, P. M. Murphy, et al. Activation of Cutaneous Protein Kinase C{alpha} Induces Keratinocyte Apoptosis and Intraepidermal Inflammation by Independent Signaling Pathways J. Immunol., September 1, 2003; 171(5): 2703 - 2713. [Abstract] [Full Text] [PDF]

				K. C. Nabbe, P. L. van Lent, A. E. Holthuysen, J. K. Kolls, S. Verbeek, and W. B. van den Berg Fc{gamma}RI Up-Regulation Induced by Local Adenoviral-Mediated Interferon-{gamma} Production Aggravates Chondrocyte Death during Immune Complex-Mediated Arthritis Am. J. Pathol., August 1, 2003; 163(2): 743 - 752. [Abstract] [Full Text] [PDF]

				R. B. Henderson, J. A. R. Hobbs, M. Mathies, and N. Hogg Rapid recruitment of inflammatory monocytes is independent of neutrophil migration Blood, July 1, 2003; 102(1): 328 - 335. [Abstract] [Full Text] [PDF]