| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine and Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210
We previously reported that activation of the phosphatidylinositol (PI) 3-kinase pathway was important in M-CSF-induced monocyte survival. Because M-CSF also induces activation of the mitogen-activated protein (MAP) kinase extracellular-regulated kinase (Erk), we focused on dissecting the mechanism used by M-CSF to induce Erk activation in human monocytes. We found that, in addition to the MAP/Erk kinase inhibitor PD098059, the PI 3-kinase inhibitors LY294002 and wortmannin both suppressed Erk activation in M-CSF-treated monocytes, suggesting that 3-phosphorylated products of PI 3-kinase played a role in Erk activation. Investigating the biochemical pathways regulated by PI 3-kinase to activate Erk, we found that, in response to M-CSF, normal human monocytes induced reactive oxygen species (ROS), which were suppressed by the PI 3-kinase inhibitor wortmannin but not by the solvent control DMSO or the MAP/Erk kinase inhibitor PD098059. We next found that, in the absence of M-CSF, ROS could induce Erk activation in human monocytes. Exogenous H2O2 induced Erk activation in human monocytes, which was suppressed by exogenous catalase. To determine whether ROS induced by M-CSF played a role in Erk activation, we found that N-acetylcysteine and diphenyleneiodonium both suppressed Erk activation in M-CSF-treated monocytes. Erk activation by M-CSF also seemed to play a role in cellular survival in monocytes. These data suggest that, in M-CSF-stimulated human monocytes, PI 3-kinase products and ROS production play a role in Erk activation and monocyte survival.
This article has been cited by other articles:
|
|
 |
|
  A. Jacquel, N. Benikhlef, J. Paggetti, N. Lalaoui, L. Guery, E. K. Dufour, M. Ciudad, C. Racoeur, O. Micheau, L. Delva, et al. Colony-stimulating factor-1-induced oscillations in phosphatidylinositol-3 kinase/AKT are required for caspase activation in monocytes undergoing differentiation into macrophages Blood, October 22, 2009; 114(17): 3633 - 3641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Lo, P. Gorak-Stolinska, V. Bachy, M. A. Ibrahim, D. M. Kemeny, and J. Maher Modulation of dendritic cell differentiation by colony-stimulating factor-1: role of phosphatidylinositol 3'-kinase and delayed caspase activation J. Leukoc. Biol., December 1, 2007; 82(6): 1446 - 1454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Okazaki, S. Ebihara, M. Asada, S. Yamanda, Y. Saijo, Y. Shiraishi, T. Ebihara, K. Niu, H. Mei, H. Arai, et al. Macrophage Colony-Stimulating Factor Improves Cardiac Function after Ischemic Injury by Inducing Vascular Endothelial Growth Factor Production and Survival of Cardiomyocytes Am. J. Pathol., October 1, 2007; 171(4): 1093 - 1103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lombardo, A. Alvarez-Barrientos, B. Maroto, L. Bosca, and U. G. Knaus TLR4-Mediated Survival of Macrophages Is MyD88 Dependent and Requires TNF-{alpha} Autocrine Signalling J. Immunol., March 15, 2007; 178(6): 3731 - 3739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zins, D. Abraham, M. Sioud, and S. Aharinejad Colon Cancer Cell-Derived Tumor Necrosis Factor-{alpha} Mediates the Tumor Growth-Promoting Response in Macrophages by Up-regulating the Colony-Stimulating Factor-1 Pathway Cancer Res., February 1, 2007; 67(3): 1038 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, M. M. Zeigler, G. K. Lam, M. G. Hunter, T. D. Eubank, V. V. Khramtsov, S. Tridandapani, C. K. Sen, and C. B. Marsh The Role of the NADPH Oxidase Complex, p38 MAPK, and Akt in Regulating Human Monocyte/Macrophage Survival Am. J. Respir. Cell Mol. Biol., January 1, 2007; 36(1): 68 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Weigert, A. M. Johann, A. von Knethen, H. Schmidt, G. Geisslinger, and B. Brune Apoptotic cells promote macrophage survival by releasing the antiapoptotic mediator sphingosine-1-phosphate Blood, September 1, 2006; 108(5): 1635 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Liu, J. L. Arbiser, A. Holmgren, G. Klein, and E. Klein PSK and Trx80 inhibit B-cell growth in EBV-infected cord blood mononuclear cells through T cells activated by the monocyte products IL-15 and IL-12 Blood, February 15, 2005; 105(4): 1606 - 1613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hayashi, H. Ohnishi, H. Okazawa, S. Nakazawa, H. Ikeda, S.-i. Motegi, N. Aoki, S. Kimura, M. Mikuni, and T. Matozaki Positive Regulation of Phagocytosis by SIRP{beta} and Its Signaling Mechanism in Macrophages J. Biol. Chem., July 9, 2004; 279(28): 29450 - 29460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Zeigler, A. I. Doseff, M. F. Galloway, J. M. Opalek, P. T. Nowicki, J. L. Zweier, C. K. Sen, and C. B. Marsh Presentation of Nitric Oxide Regulates Monocyte Survival through Effects on Caspase-9 and Caspase-3 Activation J. Biol. Chem., April 4, 2003; 278(15): 12894 - 12902. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
