| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Division of Infectious Diseases, Childrens Hospital, and Keck School of Medicine, University of Southern California, Los Angeles, CA 90027;
Department of Clinical Chemistry, Lund University, University Hospital Malmo, Malmo, Sweden; and
Institut National de la Santé et de la Recherche Médicale, Unité 428 University Paris V, Paris, France
Escherichia coli is an important pathogen that
causes meningitis in neonates. The development of bacteremia preceding
the traversal across the blood-brain barrier is a prerequisite for this
pathogen that obviously must survive the bactericidal activity of
serum. Here we report that outer membrane protein A (OmpA) of
Escherichia coli contributes to serum resistance by
binding to C4b binding protein (C4bp), a complement fluid phase
regulator. C4bp contains seven identical 
-chains and one 
-chain
linked together with disulfide bridges. We found that OmpA binds the
-chain of C4bp, which is composed of eight homologous complement
control protein (CCP) modules. Binding studies using mutants of
recombinant C4bp that lack one CCP at a time suggest that CCP3 is the
major site of interaction with OmpA. Furthermore, we demonstrate that
the N terminus of OmpA interacts with C4bp. Binding of C4bp to OmpA is
not significantly inhibited in the presence of either C4b or heparin
and is not salt sensitive, implying that it is hydrophobic in nature,
suggesting a novel interaction between OmpA and C4bp. A compelling
observation in this study is that synthetic peptides corresponding to
CCP3 sequences block the binding of C4bp to OmpA and also significantly
enhance serum bactericidal activity.
This article has been cited by other articles:
|
|
 |
|
  M. Potempa, J. Potempa, M. Okroj, K. Popadiak, S. Eick, K.-A. Nguyen, K. Riesbeck, and A. M. Blom Binding of Complement Inhibitor C4b-Binding Protein Contributes to Serum Resistance of Porphyromonas gingivalis J. Immunol., October 15, 2008; 181(8): 5537 - 5544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mittal and N. V. Prasadarao Outer Membrane Protein A Expression in Escherichia coli K1 Is Required to Prevent the Maturation of Myeloid Dendritic Cells and the Induction of IL-10 and TGF-{beta} J. Immunol., August 15, 2008; 181(4): 2672 - 2682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hallstrom, P. F. Zipfel, A. M. Blom, N. Lauer, A. Forsgren, and K. Riesbeck Haemophilus influenzae Interacts with the Human Complement Inhibitor Factor H J. Immunol., July 1, 2008; 181(1): 537 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Fagan, M. A. Lambert, and S. G. J. Smith The Hek Outer Membrane Protein of Escherichia coli Strain RS218 Binds to Proteoglycan and Utilizes a Single Extracellular Loop for Adherence, Invasion, and Autoaggregation Infect. Immun., March 1, 2008; 76(3): 1135 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ram, J. Ngampasutadol, A. D. Cox, A. M. Blom, L. A. Lewis, F. St. Michael, J. Stupak, S. Gulati, and P. A. Rice Heptose I Glycan Substitutions on Neisseria gonorrhoeae Lipooligosaccharide Influence C4b-Binding Protein Binding and Serum Resistance Infect. Immun., August 1, 2007; 75(8): 4071 - 4081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jarva, J. Ngampasutadol, S. Ram, P. A. Rice, B. O. Villoutreix, and A. M. Blom Molecular Characterization of the Interaction between Porins of Neisseria gonorrhoeae and C4b-Binding Protein J. Immunol., July 1, 2007; 179(1): 540 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hallstrom, H. Jarva, K. Riesbeck, and A. M. Blom Interaction with C4b-Binding Protein Contributes to Nontypeable Haemophilus influenzae Serum Resistance J. Immunol., May 15, 2007; 178(10): 6359 - 6366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Marodi Neonatal Innate Immunity to Infectious Agents Infect. Immun., April 1, 2006; 74(4): 1999 - 2006. [Full Text] [PDF]
|
|
|
|
|
|
A. S. Attia, S. Ram, P. A. Rice, and E. J. Hansen Binding of Vitronectin by the Moraxella catarrhalis UspA2 Protein Interferes with Late Stages of the Complement Cascade Infect. Immun., March 1, 2006; 74(3): 1597 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. T. Jenkins, L. Mark, G. Ball, J. Persson, G. Lindahl, D. Uhrin, A. M. Blom, and P. N. Barlow Human C4b-binding Protein, Structural Basis for Interaction with Streptococcal M Protein, a Major Bacterial Virulence Factor J. Biol. Chem., February 10, 2006; 281(6): 3690 - 3697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Chromy, M. W. Choi, G. A. Murphy, A. D. Gonzales, C. H. Corzett, B. C. Chang, J. P. Fitch, and S. L. McCutchen-Maloney Proteomic Characterization of Yersinia pestis Virulence J. Bacteriol., December 1, 2005; 187(23): 8172 - 8180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ngampasutadol, S. Ram, A. M. Blom, H. Jarva, A. E. Jerse, E. Lien, J. Goguen, S. Gulati, and P. A. Rice From The Cover: Human C4b-binding protein selectively interacts with Neisseria gonorrhoeae and results in species-specific infection PNAS, November 22, 2005; 102(47): 17142 - 17147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nordstrom, A. M. Blom, T. T. Tan, A. Forsgren, and K. Riesbeck Ionic Binding of C3 to the Human Pathogen Moraxella catarrhalis Is a Unique Mechanism for Combating Innate Immunity J. Immunol., September 15, 2005; 175(6): 3628 - 3636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jarva, S. Ram, U. Vogel, A. M. Blom, and S. Meri Binding of the Complement Inhibitor C4bp to Serogroup B Neisseria meningitidis J. Immunol., May 15, 2005; 174(10): 6299 - 6307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Meri, A. M. Blom, A. Hartmann, D. Lenk, S. Meri, and P. F. Zipfel The Hyphal and Yeast Forms of Candida albicans Bind the Complement Regulator C4b-Binding Protein Infect. Immun., November 1, 2004; 72(11): 6633 - 6641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Sukumaran, S. K. Selvaraj, and N. V. Prasadarao Inhibition of Apoptosis by Escherichia coli K1 Is Accompanied by Increased Expression of BclXL and Blockade of Mitochondrial Cytochrome c Release in Macrophages Infect. Immun., October 1, 2004; 72(10): 6012 - 6022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nordstrom, A. M. Blom, A. Forsgren, and K. Riesbeck The Emerging Pathogen Moraxella catarrhalis Interacts with Complement Inhibitor C4b Binding Protein through Ubiquitous Surface Proteins A1 and A2 J. Immunol., October 1, 2004; 173(7): 4598 - 4606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kask, B. O. Villoutreix, M. Steen, B. Ramesh, B. Dahlback, and A. M. Blom Structural stability and heat-induced conformational change of two complement inhibitors: C4b-binding protein and factor H Protein Sci., May 1, 2004; 13(5): 1356 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Holm, S. L. Vanlerberg, I. M. Foley, D. D. Sledjeski, and E. R. Lafontaine The Moraxella catarrhalis Porin-Like Outer Membrane Protein CD Is an Adhesin for Human Lung Cells Infect. Immun., April 1, 2004; 72(4): 1906 - 1913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Blom, B. O. Villoutreix, and B. Dahlback Mutations in {alpha}-Chain of C4BP That Selectively Affect Its Factor I Cofactor Function J. Biol. Chem., October 31, 2003; 278(44): 43437 - 43442. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
