The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dellacasagrande, J.
Right arrow Articles by Mege, J.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dellacasagrande, J.
Right arrow Articles by Mege, J.-L.
The Journal of Immunology, 2002, 169: 6309-6315.
Copyright © 2002 by The American Association of Immunologists

IFN-{gamma}-Induced Apoptosis and Microbicidal Activity in Monocytes Harboring the Intracellular Bacterium Coxiella burnetii Require Membrane TNF and Homotypic Cell Adherence1

Jérôme Dellacasagrande2, Eric Ghigo, Didier Raoult, Christian Capo and Jean-Louis Mege

Unité des Rickettsies, Unité Mixte de Recherche 6020, Centre National de la Recherche Scientifique, Faculté de Médecine, Université de la Méditerranée, Marseille, France

IFN-{gamma} is critical for the protection against intracellular bacteria through activation of the antimicrobial machinery of phagocytes. Coxiella burnetii, the etiological agent of Q fever, is a strictly intracellular bacterium that inhabits monocytes/macrophages. We previously showed that IFN-{gamma} induced C. burnetii killing by promoting the apoptosis of infected monocytes. We show in this study that IFN-{gamma}-induced apoptosis of infected monocytes was characterized by a time- and dose-dependent activation of caspase-3. IFN-{gamma}-mediated caspase-3 activation and C. burnetii killing depend on the expression of membrane TNF. Indeed, TNF was transiently expressed on the cell surface of infected monocytes a few hours after IFN-{gamma} treatment. In addition, anti-TNF Abs inhibited IFN-{gamma}-mediated caspase-3 activation whereas soluble TNF had no effect on infected cells. Concomitantly, IFN-{gamma} induced homotypic adherence of C. burnetii-infected monocytes. The latter required the interaction of {beta}2 integrins with CD54. When adherence was disrupted by pipetting, by a combination of Abs specific for CD11b, CD18, and CD54, or by an antisense oligonucleotide targeting CD18 mRNA, both cell apoptosis and bacterial killing induced by IFN-{gamma} were inhibited. Thus, adherence via CD54/{beta}2 integrins together with membrane TNF are required to eliminate C. burnetii-infected cells through cell contact-dependent apoptosis. Our results reveal a new component of the antimicrobial arsenal mobilized by IFN-{gamma} against infection by intracellular bacteria.




This article has been cited by other articles:


Home page
 QJMHome page
B.P. Marmion, O. Sukocheva, P.A. Storm, M. Lockhart, M. Turra, T. KoK, J. Ayres, H. Routledge, and S. Graves
Q fever: persistence of antigenic non-viable cell residues of Coxiella burnetii in the host--implications for post Q fever infection fatigue syndrome and other chronic sequelae
QJM, June 25, 2009; (2009) hcp077v1.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. E. Voth and R. A. Heinzen
Sustained Activation of Akt and Erk1/2 Is Required for Coxiella burnetii Antiapoptotic Activity
Infect. Immun., January 1, 2009; 77(1): 205 - 213.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Ochoa-Reparaz, J. Sentissi, T. Trunkle, C. Riccardi, and D. W. Pascual
Attenuated Coxiella burnetii Phase II Causes a Febrile Response in Gamma Interferon Knockout and Toll-Like Receptor 2 Knockout Mice and Protects against Reinfection
Infect. Immun., December 1, 2007; 75(12): 5845 - 5858.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. C. Cowley, J. D. Sedgwick, and K. L. Elkins
Differential Requirements by CD4+ and CD8+ T Cells for Soluble and Membrane TNF in Control of Francisella tularensis Live Vaccine Strain Intramacrophage Growth
J. Immunol., December 1, 2007; 179(11): 7709 - 7719.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. E. Voth, D. Howe, and R. A. Heinzen
Coxiella burnetii Inhibits Apoptosis in Human THP-1 Cells and Monkey Primary Alveolar Macrophages
Infect. Immun., September 1, 2007; 75(9): 4263 - 4271.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Andoh, G. Zhang, K. E. Russell-Lodrigue, H. R. Shive, B. R. Weeks, and J. E. Samuel
T Cells Are Essential for Bacterial Clearance, and Gamma Interferon, Tumor Necrosis Factor Alpha, and B Cells Are Crucial for Disease Development in Coxiella burnetii Infection in Mice
Infect. Immun., July 1, 2007; 75(7): 3245 - 3255.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. S. Zamboni and M. Rabinovitch
Phagocytosis of Apoptotic Cells Increases the Susceptibility of Macrophages to Infection with Coxiella burnetii Phase II through Down-Modulation of Nitric Oxide Production
Infect. Immun., April 1, 2004; 72(4): 2075 - 2080.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Capo, A. Moynault, Y. Collette, D. Olive, E. J. Brown, D. Raoult, and J.-L. Mege
Coxiella burnetii Avoids Macrophage Phagocytosis by Interfering with Spatial Distribution of Complement Receptor 3
J. Immunol., April 15, 2003; 170(8): 4217 - 4225.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.