HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Borrego, F. Articles by Coligan, J. E. Search for Related Content PubMed PubMed Citation Articles by Borrego, F. Articles by Coligan, J. E. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene

NK Cell CD94/NKG2A Inhibitory Receptors Are Internalized and Recycle Independently of Inhibitory Signaling Processes

Receptor Cell Biology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852

Human CD94/NKG2A is an inhibitory receptor that recognizes HLA-E and is expressed by NK cells and a subset of T cells. We have analyzed the cellular trafficking of the CD94/NKG2A receptor using the NKL cell line and peripheral blood NK cells. Flow cytometric, confocal microscopic, and biochemical analyses show that CD94/NKG2A continuously recycles in an active process that requires the cytoskeleton between the cell surface and intracellular compartments that are distinguishable from recycling compartments used by well-characterized receptors, such as transferrin receptor (CD71). CD94/NKG2A, an inhibitory receptor, traffics differently from the closely related CD94/NKG2C molecule, an activating receptor. Using transfection/expression analyses of wild-type and mutant CD94/NKG2A molecules in the HLA-E negative rat basophilic cell line RBL-2H3, we demonstrate that CD94/NKG2A internalization is independent of ligand cross-linking or the presence of functional immunoreceptor tyrosine-based inhibition motifs. Thus, the mechanisms that control cell surface homeostasis of CD94/NKG2A are independent of functional signaling.

This article has been cited by other articles:

				S. Coupel, A. Moreau, M. Hamidou, V. Horejsi, J.-P. Soulillou, and B. Charreau Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation Blood, April 1, 2007; 109(7): 2806 - 2814. [Abstract] [Full Text] [PDF]

				J. R. Theriault, H. Adachi, and S. K. Calderwood Role of Scavenger Receptors in the Binding and Internalization of Heat Shock Protein 70 J. Immunol., December 15, 2006; 177(12): 8604 - 8611. [Abstract] [Full Text] [PDF]

				M. Masilamani, C. Nguyen, J. Kabat, F. Borrego, and J. E. Coligan CD94/NKG2A Inhibits NK Cell Activation by Disrupting the Actin Network at the Immunological Synapse J. Immunol., September 15, 2006; 177(6): 3590 - 3596. [Abstract] [Full Text] [PDF]

				D. Kabelitz, D. Wesch, E. Pitters, and M. Zoller Characterization of Tumor Reactivity of Human V{gamma}9V{delta}2 {gamma}{delta} T Cells In Vitro and in SCID Mice In Vivo J. Immunol., December 1, 2004; 173(11): 6767 - 6776. [Abstract] [Full Text] [PDF]

				R. Gastpar, C. Gross, L. Rossbacher, J. Ellwart, J. Riegger, and G. Multhoff The Cell Surface-Localized Heat Shock Protein 70 Epitope TKD Induces Migration and Cytolytic Activity Selectively in Human NK Cells J. Immunol., January 15, 2004; 172(2): 972 - 980. [Abstract] [Full Text] [PDF]

				L. D. Lieto, F. Borrego, C.-h. You, and J. E. Coligan Human CD94 Gene Expression: Dual Promoters Differing in Responsiveness to IL-2 or IL-15 J. Immunol., November 15, 2003; 171(10): 5277 - 5286. [Abstract] [Full Text] [PDF]