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Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139
Introduction of small interfering RNAs (siRNAs) into a cell
can cause a specific interference of gene expression known as RNA
interference (RNAi). However, RNAi activity in lymphocytes and in
normal primary mammalian cells has not been thoroughly demonstrated. In
this report, we show that siRNAs complementary to CD4 and CD8
specifically reduce surface expression of these coreceptors and their
respective mRNA in a thymoma cell line model. We show that RNAi
activity is only caused by a subset of siRNAs complementary to the mRNA
target and that ineffective siRNAs can compete with effective siRNAs.
Using primary differentiated T lymphocytes, we provide the first
evidence of siRNA-mediated RNAi gene silencing in normal nontransformed
somatic mammalian lymphocytes.
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