HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosbottom, A. Articles by Miller, H. R. P. Search for Related Content PubMed PubMed Citation Articles by Rosbottom, A. Articles by Miller, H. R. P.

TGF-1 Regulates Adhesion of Mucosal Mast Cell Homologues to Laminin-1 Through Expression of Integrin ₇¹

Anne Rosbottom^*, Cheryl L. Scudamore^2,*, Helga von der Mark, Elizabeth M. Thornton, Steven H. Wright and Hugh R. P. Miller^3,

Departments of ^* Veterinary Pathology and Veterinary Clinical Studies, University of Edinburgh, Roslin, Midlothian, United Kingdom; and Institute of Experimental Medicine, Friedrich Alexander University, Erlangen, Germany

Mucosal mast cells (MMC) or their precursors migrate through the intestinal lamina propria to reside intraepithelially, where expression of mouse mast cell protease-1 indicates the mature phenotype. Alterations in expression of integrins that govern cell adhesion to the extracellular matrix may regulate this process. As the key cytokine mediating differentiation of mouse mast cell protease-1-expressing MMC homologues in vitro, TGF-1 was considered a likely candidate for regulation of the integrins that facilitate intraepithelial migration of MMC. Therefore, we examined adhesion of bone marrow-derived mast cells cultured with and without TGF-1 to laminin-1, fibronectin, and vitronectin along with expression of integrins likely to regulate this adhesion. Adhesion of PMA-stimulated cultured mast cells to laminin-1 increased from 5.3 ± 3.6% (mean ± SEM) in the absence of TGF-1 to 58.7 ± 4.0% (p < 0.05) when cultured mast cells had differentiated into MMC homologues in the presence of TGF-1_. Increased adhesion of MMC homologues to laminin-1 was also stimulated by FcRI cross-linking and the calcium ionophore A23187. Expression of the laminin-binding integrin ₇ by MMC homologues grown in the presence of TGF-1 was demonstrated by RT-PCR and flow cytometry, and preincubation of MMC homologues with the ₇-neutralizing Ab 6A11 inhibited adhesion to laminin-1 by 98% (p < 0.05), demonstrating a novel role for this molecule in adhesion of a hemopoietic cell to laminin-1.

This article has been cited by other articles:

				J.-T. Chao, L. A. Martinez-Lemus, S. J. Kaufman, G. A. Meininger, K. S. Ramos, and E. Wilson Modulation of {alpha}7-integrin-mediated adhesion and expression by platelet-derived growth factor in vascular smooth muscle cells Am J Physiol Cell Physiol, April 1, 2006; 290(4): C972 - C980. [Abstract] [Full Text] [PDF]

				T. Oki, J. Kitaura, K. Eto, Y. Lu, M. Maeda-Yamamoto, N. Inagaki, H. Nagai, Y. Yamanishi, H. Nakajina, H. Kumagai, et al. Integrin {alpha}IIb{beta}3 Induces the Adhesion and Activation of Mast Cells through Interaction with Fibrinogen J. Immunol., January 1, 2006; 176(1): 52 - 60. [Abstract] [Full Text] [PDF]

				J. K. Brown, P. A. Knight, A. D. Pemberton, S. H. Wright, J. A. Pate, E. M. Thornton, and H. R. P. Miller Expression of Integrin-{alpha}E by Mucosal Mast Cells in the Intestinal Epithelium and Its Absence in Nematode-Infected Mice Lacking the Transforming Growth Factor-{beta}1-Activating Integrin {alpha}v{beta}6 Am. J. Pathol., July 1, 2004; 165(1): 95 - 106. [Abstract] [Full Text] [PDF]