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Altered Neutrophil Trafficking During Sepsis¹

Ren-Feng Guo^*, Niels C. Riedemann^*, Ines J. Laudes^*, Vidya J. Sarma^*, Robin G. Kunkel^*, Kari A. Dilley^*, Joseph D. Paulauskis and Peter A. Ward^2,*

^* Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109; and Department of Drug Safety Evaluation, Pfizer Global Research and Development, Ann Arbor, MI 48105

In sepsis, dysregulation of the inflammatory system is well known, as reflected in excessive inflammatory mediator production, complement activation, and appearance of defects in phagocytic cells. In the current study sepsis was induced in rats by cecal ligation/puncture. Early in sepsis the ₁ and ₂ integrin content on blood neutrophils increased in a nontranscriptional manner, and the increase in ₂, but not ₁, integrin content was C5a dependent. Similar changes could be induced in vitro on blood neutrophils following contact with phorbol ester or C5a. Direct injury of lungs of normal rats induced by deposition of IgG immune complexes (IgG-IC) caused 5-fold increases in the myeloperoxidase content that was ₂, but not ₁, dependent. In contrast, in cecal ligation/puncture lungs myeloperoxidase increased 10-fold after IgG immune complex deposition and was both ₁ and ₂ integrin dependent. These data suggest that sepsis causes enhanced neutrophil trafficking into the lung via mechanisms that are not engaged in the nonseptic state.

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