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Laboratory of Cellular Differentiation, Department of Stem Cell Biology, Lund University, Lund, Sweden
The early B cell factor (EBF) is a transcription factor shown
crucial for the development of B lymphocytes. The protein is expressed
from the earliest stages of B cell development until the mature B cell
stage, but the control elements responsible for the regulation of the
gene are unknown. In this study, we report of the identification of a
promoter region flanking the EBF gene. Several
transcription start sites were identified by primer extension analysis
in a region 
3.1 kb from the predicted ATG. Transient
transfections revealed that this region was able to stimulate
transcription of a reporter gene in B lymphoid and to a lesser extent,
myeloid cells, but not in a pre-T cell line. The promoter was also able
to functionally interact with E47, suggesting that the EBF gene may be
a direct target for activation by E-proteins. In addition, functional
binding of EBF to its own promoter was confirmed by EMSA and
transfection assays indicating that the EBF protein may be involved in
an autoregulatory loop. Finally, a tissue-restricted factor was able to
bind an upstream regulatory region in B-lineage cells, further
supporting the idea that the cloned promoter participates in the
regulation of stage and lineage specific expression of the EBF
gene.
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