HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hwang, E. S. Articles by Ho, I-C. Search for Related Content PubMed PubMed Citation Articles by Hwang, E. S. Articles by Ho, I-C.

Transcriptional Regulation of GATA-3 by an Intronic Regulatory Region and Fetal Liver Zinc Finger Protein 1¹

Eun Sook Hwang^*, Andrew Choi^* and I-Cheng Ho^2,*,

^* Department of Immunology and Infectious Diseases, Harvard School of Public Health, and Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115

GATA-3 is a T cell-specific transcription factor and is essential for the development of the T cell lineage. The transcriptional regulation of GATA-3, however, remains elusive. In this study, we report the identification of a regulatory region located within the first intron of the murine GATA-3 gene. The intronic regulatory region contains both a positive and a negative cis-acting element but, as a whole, serves as a potent T cell-specific enhancer and is essential for the promoter activity in vitro. By using yeast one-hybrid screening, we discovered that fetal liver zinc finger protein 1 (Fliz1) could bind specifically to the negative cis-acting element, the sequence of which is conserved between the mouse and human GATA-3 genes. More importantly, overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. Our data suggest that the expression of GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion.

This article has been cited by other articles:

				A. C. Liberman, J. Druker, D. Refojo, F. Holsboer, and E. Arzt Glucocorticoids inhibit GATA-3 phosphorylation and activity in T cells FASEB J, May 1, 2009; 23(5): 1558 - 1571. [Abstract] [Full Text] [PDF]

				E. J. Scheinman and O. Avni Transcriptional Regulation of Gata3 in T Helper Cells by the Integrated Activities of Transcription Factors Downstream of the Interleukin-4 Receptor and T Cell Receptor J. Biol. Chem., January 30, 2009; 284(5): 3037 - 3048. [Abstract] [Full Text] [PDF]

				M. C. Lawrence, K. McGlynn, B. Naziruddin, M. F. Levy, and M. H. Cobb Inaugural Article: Differential regulation of CHOP-10/GADD153 gene expression by MAPK signaling in pancreatic beta-cells PNAS, July 10, 2007; 104(28): 11518 - 11525. [Abstract] [Full Text] [PDF]

				A. C. Liberman, D. Refojo, J. Druker, M. Toscano, T. Rein, F. Holsboer, and E. Arzt The activated glucocorticoid receptor inhibits the transcription factor T-bet by direct protein-protein interaction FASEB J, April 1, 2007; 21(4): 1177 - 1188. [Abstract] [Full Text] [PDF]

				R. A. Corn, C. Hunter, H.-C. Liou, U. Siebenlist, and M. R. Boothby Opposing Roles for RelB and Bcl-3 in Regulation of T-Box Expressed in T Cells, GATA-3, and Th Effector Differentiation J. Immunol., August 15, 2005; 175(4): 2102 - 2110. [Abstract] [Full Text] [PDF]

				S. A. Jenks, B. J. Eisfelder, and J. Miller LFA-1 co-stimulation inhibits Th2 differentiation by down-modulating IL-4 responsiveness Int. Immunol., March 1, 2005; 17(3): 315 - 323. [Abstract] [Full Text] [PDF]

				R. Barthel and A. E. Goldfeld T Cell-Specific Expression of the Human TNF-{alpha} Gene Involves a Functional and Highly Conserved Chromatin Signature in Intron 3 J. Immunol., October 1, 2003; 171(7): 3612 - 3619. [Abstract] [Full Text] [PDF]