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The Journal of Immunology, 2002, 169: 204-209.
Copyright © 2002 by The American Association of Immunologists

Functional Human T Lymphocyte Development from Cord Blood CD34+ Cells in Nonobese Diabetic/Shi-scid, IL-2 Receptor {gamma} Null Mice1

Takashi Yahata*, Kiyoshi Ando2,*,{dagger}, Yoshihiko Nakamura*, Yoshito Ueyama{ddagger},§, Kazuo Shimamura{ddagger}, Norikazu Tamaoki§, Shunichi Kato* and Tomomitsu Hotta{dagger}

* Research Center for Cell Transplantation, and Departments of {dagger} Hematology and {ddagger} Pathology, Tokai University, School of Medicine, Isehara, Kanagawa, Japan; and § Central Institute for Experimental Animal, Kawasaki, Kanagawa, Japan

An experimental model for human T lymphocyte development from hemopoietic stem cells is necessary to study the complex processes of T cell differentiation in vivo. In this study, we report a newly developed nonobese diabetic (NOD)/Shi-scid, IL-2R{gamma} null (NOD/SCID/{gamma}cnull) mouse model for human T lymphopoiesis. When these mice were transplanted with human cord blood CD34+ cells, the mice reproductively developed human T cells in their thymus and migrated into peripheral lymphoid organs. Furthermore, these T cells bear polyclonal TCR-{alpha}{beta}, and respond not only to mitogenic stimuli, such as PHA and IL-2, but to allogenic human cells. These results indicate that functional human T lymphocytes can be reconstituted from CD34+ cells in NOD/SCID/{gamma}cnull mice. This newly developed mouse model is expected to become a useful tool for the analysis of human T lymphopoiesis and immune response, and an animal model for studying T lymphotropic viral infections, such as HIV.




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