| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
* Pathology, and
Physiology and Cell Biology, Ohio State University College of Medicine and Public Health, Columbus, OH 43210;
Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, FL 33136
Workshop. C.M.C. was a Presidential Fellow at Ohio State University. D.M.M. was a Howard Hughes Medical Institute Predoctoral Fellow.
CD8+ and CD4+ T lymphocytes are important in controlling human CMV (HCMV) infection, but the virus has evolved protean mechanisms to inhibit MHC-based Ag presentation and escape T lymphocyte immunosurveillance. Herein, the interaction of HCMV with the MHC class II Ag presentation pathway was investigated in cells stably transfected with class II transactivator. Flow cytometry experiments demonstrate that HCMV infection decreases cell-surface MHC class II expression. HCMV down-regulates MHC class II surface expression without a significant effect on class II RNA or steady-state protein levels. SDS-stability and confocal microscopy experiments demonstrate normal levels of steady-state peptide-loaded class II molecules in infected cells and that class II molecules reach late endosomal and HLA-DM positive peptide-loading compartments. However, MHC class II positive vesicles are retained in an abnormal perinuclear distribution. Finally, experiments with a mutant HCMV strain demonstrate that this novel mechanism of decreased MHC class II expression is not mediated by one of the known HCMV immunomodulatory genes. These defects in MHC class II expression combined with previously identified CMV strategies for decreasing MHC class I expression enables infected cells to evade T lymphocyte immunosurveillance.
This article has been cited by other articles:
|
|
 |
|
  S. Delmas, P. Brousset, D. Clement, E. Le Roy, and J.-L. Davignon Anti-IE1 CD4+ T-cell clones kill peptide-pulsed, but not human cytomegalovirus-infected, target cells J. Gen. Virol., September 1, 2007; 88(9): 2441 - 2449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Wiertz, R. Devlin, H. L. Collins, and M. E. Ressing Herpesvirus Interference with Major Histocompatibility Complex Class II-Restricted T-Cell Activation J. Virol., May 1, 2007; 81(9): 4389 - 4396. [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Lee, L. Hertel, R. K. Louie, T. Burster, V. Lacaille, A. Pashine, D. A. Abate, E. S. Mocarski, and E. D. Mellins Human Cytomegalovirus Alters Localization of MHC Class II and Dendrite Morphology in Mature Langerhans Cells J. Immunol., September 15, 2006; 177(6): 3960 - 3971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sinzger, K. Eberhardt, Y. Cavignac, C. Weinstock, T. Kessler, G. Jahn, and J.-L. Davignon Macrophage cultures are susceptible to lytic productive infection by endothelial-cell-propagated human cytomegalovirus strains and present viral IE1 protein to CD4+ T cells despite late downregulation of MHC class II molecules J. Gen. Virol., July 1, 2006; 87(7): 1853 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Trgovcich, C. Cebulla, P. Zimmerman, and D. D. Sedmak Human Cytomegalovirus Protein pp71 Disrupts Major Histocompatibility Complex Class I Cell Surface Expression J. Virol., January 15, 2006; 80(2): 951 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Li, N. Wang, D. Zhou, C. S. K. Yee, C.-H. Chang, R. R. Brutkiewicz, and J. S. Blum Disruption of MHC Class II-Restricted Antigen Presentation by Vaccinia Virus J. Immunol., November 15, 2005; 175(10): 6481 - 6488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Prechtel, N. M. Turza, D. J. Kobelt, J. I. Eisemann, R. S. Coffin, Y. McGrath, C. Hacker, X. Ju, M. Zenke, and A. Steinkasserer Infection of mature dendritic cells with herpes simplex virus type 1 dramatically reduces lymphoid chemokine-mediated migration J. Gen. Virol., June 1, 2005; 86(6): 1645 - 1657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zimmermann, M. Trilling, M. Wagner, M. Wilborn, I. Bubic, S. Jonjic, U. Koszinowski, and H. Hengel A cytomegaloviral protein reveals a dual role for STAT2 in IFN-{gamma} signaling and antiviral responses J. Exp. Med., May 16, 2005; 201(10): 1543 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Hegde and D. C. Johnson Human Cytomegalovirus US2 Causes Similar Effects on Both Major Histocompatibility Complex Class I and II Proteins in Epithelial and Glial Cells J. Virol., September 1, 2003; 77(17): 9287 - 9294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hertel, V. G. Lacaille, H. Strobl, E. D. Mellins, and E. S. Mocarski Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus J. Virol., July 1, 2003; 77(13): 7563 - 7574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baillie, D. A. Sahlender, and J. H. Sinclair Human Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha (TNF-{alpha}) Signaling by Targeting the 55-Kilodalton TNF-{alpha} Receptor J. Virol., June 15, 2003; 77(12): 7007 - 7016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Odeberg, B. Plachter, L. Branden, and C. Soderberg-Naucler Human cytomegalovirus protein pp65 mediates accumulation of HLA-DR in lysosomes and destruction of the HLA-DR {alpha}-chain Blood, June 15, 2003; 101(12): 4870 - 4877. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
