The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeng, R.
Right arrow Articles by Watanabe, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeng, R.
Right arrow Articles by Watanabe, S.
The Journal of Immunology, 2002, 168: 4567-4575.
Copyright © 2002 by The American Association of Immunologists

Stat5B Shuttles Between Cytoplasm and Nucleus in a Cytokine-Dependent and -Independent Manner1

Rong Zeng*,{ddagger}, Yutaka Aoki*,{ddagger}, Minoru Yoshida{dagger},{ddagger}, Ken-ichi Arai*,{ddagger} and Sumiko Watanabe2,*

* Department of Molecular and Developmental Biology, Institute of Medical Science, and {dagger} Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo, Japan; and {ddagger} Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo, Japan

In response to cytokine stimuli, Stats are phosphorylated and translocated to the nucleus to activate target genes. Then, most are dephosphorylated and returned to the cytoplasm. Using Ba/F3 cells, we found that the nuclear export of Stat5B by cytokine depletion was inhibited by leptomycin B (LMB), a specific inhibitor of nuclear export receptor chromosome region maintenance 1. Interestingly, LMB treatment in the absence of cytokine led to the accumulation of Stat5B in the nucleus, suggesting that Stat5B shuttles between the nucleus and the cytoplasm as a monomer without cytokine stimulation. This notion is supported by the observation that LMB-induced accumulation of Stat5B in the nucleus was also observed with Stat5B having a mutated tyrosine 699, which is essential for dimer formation. Using a series of mutant Stat5Bs, we identified a part of the coiled coil domain to be a critical region for monomer nuclear import and a more N-terminal region to be critical for the cytokine stimulation dependent import of Stat5B. Taken together, we propose a model in which Stat5B shuttles between the nucleus and cytoplasm by two different mechanisms, one being a factor-independent constitutive shuttling by monomeric form, and the other, a factor stimulation-dependent one regulated by tyrosine phosphorylation and subsequent dimerization.




This article has been cited by other articles:


Home page
 J. Virol.Home page
L. Vazquez-Iglesias, I. Lostale-Seijo, J. Martinez-Costas, and J. Benavente
Avian Reovirus SigmaA Localizes to the Nucleolus and Enters the Nucleus by a Nonclassical Energy- and Carrier-Independent Pathway
J. Virol., October 1, 2009; 83(19): 10163 - 10175.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Kawashima, Y. C. Bao, Y. Minoshima, Y. Nomura, T. Hatori, T. Hori, T. Fukagawa, T. Fukada, N. Takahashi, T. Nosaka, et al.
A Rac GTPase-Activating Protein, MgcRacGAP, Is a Nuclear Localizing Signal-Containing Nuclear Chaperone in the Activation of STAT Transcription Factors
Mol. Cell. Biol., April 1, 2009; 29(7): 1796 - 1813.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
S.-H. Tan and M. T Nevalainen
Signal transducer and activator of transcription 5A/B in prostate and breast cancers
Endocr. Relat. Cancer, June 1, 2008; 15(2): 367 - 390.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Basham, M. Sathe, J. Grein, T. McClanahan, A. D'Andrea, E. Lees, and A. Rascle
In vivo identification of novel STAT5 target genes
Nucleic Acids Res., June 1, 2008; 36(11): 3802 - 3818.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. Iyer and N. C. Reich
Constitutive nuclear import of latent and activated STAT5a by its coiled coil domain
FASEB J, February 1, 2008; 22(2): 391 - 400.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Herrmann, M. Vogt, M. Monnigmann, T. Clahsen, U. Sommer, S. Haan, V. Poli, P. C. Heinrich, and G. Muller-Newen
Nucleocytoplasmic shuttling of persistently activated STAT3
J. Cell Sci., September 15, 2007; 120(18): 3249 - 3261.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Kawashima, Y. C. Bao, Y. Nomura, Y. Moon, Y. Tonozuka, Y. Minoshima, T. Hatori, A. Tsuchiya, M. Kiyono, T. Nosaka, et al.
Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors
J. Cell Biol., December 18, 2006; 175(6): 937 - 946.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Chen, X. Dai, A. L. Haas, R. Wen, and D. Wang
Proteasome-dependent down-regulation of activated Stat5A in the nucleus
Blood, July 15, 2006; 108(2): 566 - 574.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
P. Fang, E. M. Kofoed, B. M. Little, X. Wang, R. J. M. Ross, S. J. Frank, V. Hwa, and R. G. Rosenfeld
A Mutant Signal Transducer and Activator of Transcription 5b, Associated with Growth Hormone Insensitivity and Insulin-Like Growth Factor-I Deficiency, Cannot Function as a Signal Transducer or Transcription Factor
J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1526 - 1534.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
X. Lu, H. Nechushtan, F. Ding, M. F. Rosado, R. Singal, A. A. Alizadeh, and I. S. Lossos
Distinct IL-4-induced gene expression, proliferation, and intracellular signaling in germinal center B-cell-like and activated B-cell-like diffuse large-cell lymphomas
Blood, April 1, 2005; 105(7): 2924 - 2932.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
U. Vinkemeier
Getting the message across, STAT! Design principles of a molecular signaling circuit
J. Cell Biol., October 25, 2004; 167(2): 197 - 201.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Marg, Y. Shan, T. Meyer, T. Meissner, M. Brandenburg, and U. Vinkemeier
Nucleocytoplasmic shuttling by nucleoporins Nup153 and Nup214 and CRM1-dependent nuclear export control the subcellular distribution of latent Stat1
J. Cell Biol., June 21, 2004; 165(6): 823 - 833.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. L. Pranada, S. Metz, A. Herrmann, P. C. Heinrich, and G. Muller-Newen
Real Time Analysis of STAT3 Nucleocytoplasmic Shuttling
J. Biol. Chem., April 9, 2004; 279(15): 15114 - 15123.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. J. Mitchell, S. J. Whittaker, and S. John
Dysregulated Expression of COOH-Terminally Truncated Stat5 and Loss of IL2-Inducible Stat5-Dependent Gene Expression in Sezary Syndrome
Cancer Res., December 15, 2003; 63(24): 9048 - 9054.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
K. M. McBride and N. C. Reich
The Ins and Outs of STAT1 Nuclear Transport
Sci. Signal., August 12, 2003; 2003(195): re13 - re13.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Haendeler, J. Hoffmann, R. P. Brandes, A. M. Zeiher, and S. Dimmeler
Hydrogen Peroxide Triggers Nuclear Export of Telomerase Reverse Transcriptase via Src Kinase Family-Dependent Phosphorylation of Tyrosine 707
Mol. Cell. Biol., July 1, 2003; 23(13): 4598 - 4610.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. Swameye, T. G. Muller, J. Timmer, O. Sandra, and U. Klingmuller
Identification of nucleocytoplasmic cycling as a remote sensor in cellular signaling by databased modeling
PNAS, February 4, 2003; 100(3): 1028 - 1033.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.