| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Cutting Edge |
Departments of
* Cell Biology and
Pathology, Duke University Medical Center, Durham, NC 27710
GRP94(gp96) elicits CD8+ T cell responses against
its bound peptides, a process requiring access of its associated
peptides into the MHC class I cross-presentation pathway of APCs. Entry
into this pathway requires receptor-mediated endocytosis, and CD91
(low-density lipoprotein receptor-related protein) has been reported to
be the receptor mediating GRP94 uptake into APC. However, a direct role
for CD91 in chaperone-based peptide Ag re-presentation has not been
demonstrated. We investigated the contribution of CD91 to GRP94 cell
surface binding, internalization, and trafficking in APCs. Whereas a
clear role for CD91 in 
2-macroglobulin binding and
uptake was readily obtained, the addition of excess CD91 ligand,
activated 2-macroglobulin, or receptor-associated
protein, an antagonist of all known CD91 ligands, did not affect GRP94
cell surface binding, receptor-mediated endocytosis, or peptide
re-presentation. These data identify a CD91-independent, GRP94
internalization pathway that functions in peptide Ag
re-presentation.
This article has been cited by other articles:
|
|
 |
|
  E. Amiel, A. Alonso, S. Uematsu, S. Akira, M. E. Poynter, and B. Berwin Pivotal Advance: Toll-like receptor regulation of scavenger receptor-A-mediated phagocytosis J. Leukoc. Biol., April 1, 2009; 85(4): 595 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Lillis, L. B. Van Duyn, J. E. Murphy-Ullrich, and D. K. Strickland LDL Receptor-Related Protein 1: Unique Tissue-Specific Functions Revealed by Selective Gene Knockout Studies Physiol Rev, July 1, 2008; 88(3): 887 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Theriault, H. Adachi, and S. K. Calderwood Role of Scavenger Receptors in the Binding and Internalization of Heat Shock Protein 70 J. Immunol., December 15, 2006; 177(12): 8604 - 8611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Graner and D. D. Bigner Chaperone proteins and brain tumors: Potential targets and possible therapeutics Neuro-oncol, July 1, 2005; 7(3): 260 - 278. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Demine and P. Walden Testing the Role of gp96 as Peptide Chaperone in Antigen Processing J. Biol. Chem., May 6, 2005; 280(18): 17573 - 17578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Berwin, Y. Delneste, R. V. Lovingood, S. R. Post, and S. V. Pizzo SREC-I, a Type F Scavenger Receptor, Is an Endocytic Receptor for Calreticulin J. Biol. Chem., December 3, 2004; 279(49): 51250 - 51257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Tobar, P. A. Gonzalez, and A. M. Kalergis Salmonella Escape from Antigen Presentation Can Be Overcome by Targeting Bacteria to Fc{gamma} Receptors on Dendritic Cells J. Immunol., September 15, 2004; 173(6): 4058 - 4065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Binder and P. K. Srivastava Essential role of CD91 in re-presentation of gp96-chaperoned peptides PNAS, April 20, 2004; 101(16): 6128 - 6133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. K. Rapp and S. H. Kaufmann DNA vaccination with gp96-peptide fusion proteins induces protection against an intracellular bacterial pathogen Int. Immunol., April 1, 2004; 16(4): 597 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Y. Chandawarkar, M. S. Wagh, J. T. Kovalchin, and P. Srivastava Immune modulation with high-dose heat-shock protein gp96: therapy of murine autoimmune diabetes and encephalomyelitis Int. Immunol., April 1, 2004; 16(4): 615 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Baker-LePain, M. Sarzotti, and C. V. Nicchitta Glucose-Regulated Protein 94/Glycoprotein 96 Elicits Bystander Activation of CD4+ T Cell Th1 Cytokine Production In Vivo J. Immunol., April 1, 2004; 172(7): 4195 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Stebbing, B. Gazzard, S. Portsmouth, F. Gotch, L. Kim, M. Bower, S. Mandalia, R. Binder, P. Srivastava, and S. Patterson Disease-associated dendritic cells respond to disease-specific antigens through the common heat shock protein receptor Blood, September 1, 2003; 102(5): 1806 - 1814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Radsak, N. Hilf, H. Singh-Jasuja, S. Braedel, P. Brossart, H.-G. Rammensee, and H. Schild The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions Blood, April 1, 2003; 101(7): 2810 - 2815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vogen, T. Gidalevitz, C. Biswas, B. B. Simen, E. Stein, F. Gulmen, and Y. Argon Radicicol-sensitive Peptide Binding to the N-terminal Portion of GRP94 J. Biol. Chem., October 18, 2002; 277(43): 40742 - 40750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Becker, F.-U. Hartl, and F. Wieland CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes J. Cell Biol., September 29, 2002; 158(7): 1277 - 1285. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
