The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cottin, V.
Right arrow Articles by Riches, D. W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cottin, V.
Right arrow Articles by Riches, D. W. H.
The Journal of Immunology, 2002, 168: 4095-4102.
Copyright © 2002 by The American Association of Immunologists

Restricted Localization of the TNF Receptor CD120a to Lipid Rafts: A Novel Role for the Death Domain1

Vincent Cottin*, Joyce E. S. Doan* and David W. H. Riches2,*,{dagger}

* Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206; and {dagger} Division of Pulmonary Sciences and Critical Care Medicine, Departments of Immunology, Medicine, and Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262

The TNF-{alpha} receptor, CD120a, has recently been shown to be localized to both plasma membrane lipid rafts and to the trans Golgi complex. Through a combination of both confocal microscopy and sucrose density gradient ultracentrifugation, we show that amino acid sequences located within the death domain (DD) of CD120a are both necessary and sufficient to promote the appropriate localization of the receptor to lipid rafts. Deletion of the DD (CD120a.{Delta}321-425) prevented the receptor from being targeted to lipid rafts and resulted in a uniform plasma membrane localization. A similar loss of raft localization was also observed following pairwise deletion of the six {alpha}-helices that comprise the DD. In all situations, the loss of the ability of CD120a to become localized to lipid rafts following mutagenesis was paralleled by a failure of the receptor to initiate apoptosis. Furthermore, introduction of the lpr mutation into CD120a (CD120a.L351N) also resulted in both a loss in the ability of the receptor to signal apoptosis and to be appropriately localized to rafts. In contrast to CD120a, CD120b, which lacks a DD, is mainly expressed in the bulk plasma membrane and to a lesser extent in lipid rafts, but is absent from the Golgi complex. However, a chimeric receptor in which the DD of CD120a was fused to the cytoplasmic domain of CD120b was predominantly localized to lipid rafts. Collectively, these findings suggest that in addition to its role in CD120a signaling, an appropriately folded and functionally active DD is required for the localization of the receptor to lipid rafts.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Paland, L. Bohme, R. K. Gurumurthy, A. Maurer, A. J. Szczepek, and T. Rudel
Reduced Display of Tumor Necrosis Factor Receptor I at the Host Cell Surface Supports Infection with Chlamydia trachomatis
J. Biol. Chem., March 7, 2008; 283(10): 6438 - 6448.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Hunter and G. F. Nixon
Spatial Compartmentalization of Tumor Necrosis Factor (TNF) Receptor 1-dependent Signaling Pathways in Human Airway Smooth Muscle Cells: LIPID RAFTS ARE ESSENTIAL FOR TNF-{alpha}-MEDIATED ACTIVATION OF RhoA BUT DISPENSABLE FOR THE ACTIVATION OF THE NF-{kappa}B AND MAPK PATHWAYS
J. Biol. Chem., November 10, 2006; 281(45): 34705 - 34715.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
K. Heo, U. Jariwala, J. Woo, Y. Zhan, K. A. Burke, L. Zhu, W. F. Anderson, and Y. Zhao
Involvement of Niemann-Pick Type C2 Protein in Hematopoiesis Regulation
Stem Cells, June 1, 2006; 24(6): 1549 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. Garcia-Marcos, E. Perez-Andres, S. Tandel, U. Fontanils, A. Kumps, E. Kabre, A. Gomez-Munoz, A. Marino, J.-P. Dehaye, and S. Pochet
Coupling of two pools of P2X7 receptors to distinct intracellular signaling pathways in rat submandibular gland
J. Lipid Res., April 1, 2006; 47(4): 705 - 714.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
W. A. Shah, H. Peng, and S. Carbonetto
Role of non-raft cholesterol in lymphocytic choriomeningitis virus infection via {alpha}-dystroglycan.
J. Gen. Virol., March 1, 2006; 87(Pt 3): 673 - 678.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Osawa, H. Uchinami, J. Bielawski, R. F. Schwabe, Y. A. Hannun, and D. A. Brenner
Roles for C16-ceramide and Sphingosine 1-Phosphate in Regulating Hepatocyte Apoptosis in Response to Tumor Necrosis Factor-{alpha}
J. Biol. Chem., July 29, 2005; 280(30): 27879 - 27887.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Rotolo, J. Zhang, M. Donepudi, H. Lee, Z. Fuks, and R. Kolesnick
Caspase-dependent and -independent Activation of Acid Sphingomyelinase Signaling
J. Biol. Chem., July 15, 2005; 280(28): 26425 - 26434.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. D'Alessio, R. S. Al-Lamki, J. R. Bradley, and J. S. Pober
Caveolae Participate in Tumor Necrosis Factor Receptor 1 Signaling and Internalization in a Human Endothelial Cell Line
Am. J. Pathol., April 1, 2005; 166(4): 1273 - 1282.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Lotocki, O. F. Alonso, W. D. Dietrich, and R. W. Keane
Tumor Necrosis Factor Receptor 1 and Its Signaling Intermediates Are Recruited to Lipid Rafts in the Traumatized Brain
J. Neurosci., December 8, 2004; 24(49): 11010 - 11016.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Scheel-Toellner, K. Wang, R. Craddock, P. R. Webb, H. M. McGettrick, L. K. Assi, N. Parkes, L. E. Clough, E. Gulbins, M. Salmon, et al.
Reactive oxygen species limit neutrophil life span by activating death receptor signaling
Blood, October 15, 2004; 104(8): 2557 - 2564.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. Fessler, P. G. Arndt, S. C. Frasch, J. G. Lieber, C. A. Johnson, R. C. Murphy, J. A. Nick, D. L. Bratton, K. C. Malcolm, and G. S. Worthen
Lipid Rafts Regulate Lipopolysaccharide-induced Activation of Cdc42 and Inflammatory Functions of the Human Neutrophil
J. Biol. Chem., September 17, 2004; 279(38): 39989 - 39998.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. E. S. Doan, D. A. Windmiller, and D. W. H. Riches
Differential Regulation of TNF-R1 Signaling: Lipid Raft Dependency of p42mapk/erk2 Activation, but Not NF-{kappa}B Activation
J. Immunol., June 15, 2004; 172(12): 7654 - 7660.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. M. Aouad, L. Y. Cohen, E. Sharif-Askari, E. K. Haddad, A. Alam, and R.-P. Sekaly
Caspase-3 Is a Component of Fas Death-Inducing Signaling Complex in Lipid Rafts and Its Activity Is Required for Complete Caspase-8 Activation during Fas-Mediated Cell Death
J. Immunol., February 15, 2004; 172(4): 2316 - 2323.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. C. Bender, J. C. Whitbeck, M. Ponce de Leon, H. Lou, R. J. Eisenberg, and G. H. Cohen
Specific Association of Glycoprotein B with Lipid Rafts during Herpes Simplex Virus Entry
J. Virol., September 1, 2003; 77(17): 9542 - 9552.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. Rodemer, T.-P. Thai, B. Brugger, T. Kaercher, H. Werner, K.-A. Nave, F. Wieland, K. Gorgas, and W. W. Just
Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice
Hum. Mol. Genet., August 1, 2003; 12(15): 1881 - 1895.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Harper, M. Hughes, M. MacFarlane, and G. M. Cohen
Fas-associated Death Domain Protein and Caspase-8 Are Not Recruited to the Tumor Necrosis Factor Receptor 1 Signaling Complex during Tumor Necrosis Factor-induced Apoptosis
J. Biol. Chem., July 3, 2003; 278(28): 25534 - 25541.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. A. Walton, A. L. Cole, M. Yeh, G. Subbanagounder, S. R. Krutzik, R. L. Modlin, R. M. Lucas, J. Nakai, E. J. Smart, D. K. Vora, et al.
Specific Phospholipid Oxidation Products Inhibit Ligand Activation of Toll-Like Receptors 4 and 2
Arterioscler. Thromb. Vasc. Biol., July 1, 2003; 23(7): 1197 - 1203.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Thomas, R. Kumar, A. Preda-Pais, S. Casares, and T.-D. Brumeanu
A Model for Antigen-Specific T-Cell Anergy: Displacement of CD4-p56lck Signalosome from the Lipid Rafts by a Soluble, Dimeric Peptide-MHC Class II Chimera
J. Immunol., June 15, 2003; 170(12): 5981 - 5992.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Ha, H. B. Kwak, S. K. Lee, D. S. Na, C. E. Rudd, Z. H. Lee, and H.-H. Kim
Membrane Rafts Play a Crucial Role in Receptor Activator of Nuclear Factor kappa B Signaling and Osteoclast Function
J. Biol. Chem., May 9, 2003; 278(20): 18573 - 18580.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X.-J. Zhao, L. Marrero, K. Song, P. Oliver, S. Y. Chin, H. Simon, J. R. Schurr, Z. Zhang, D. Thoppil, S. Lee, et al.
Acute Alcohol Inhibits TNF-{alpha} Processing in Human Monocytes by Inhibiting TNF/TNF-{alpha}-Converting Enzyme Interactions in the Cell Membrane
J. Immunol., March 15, 2003; 170(6): 2923 - 2931.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.