HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kieffer, L. J. Articles by Kavathas, P. B. Search for Related Content PubMed PubMed Citation Articles by Kieffer, L. J. Articles by Kavathas, P. B.

Identification of a Candidate Regulatory Region in the Human CD8 Gene Complex by Colocalization of DNase I Hypersensitive Sites and Matrix Attachment Regions Which Bind SATB1 and GATA-3¹

Lynda J. Kieffer^*, John M. Greally, Inna Landres^*, Shanta Nag^*, Yuko Nakajima, Terumi Kohwi-Shigematsu and Paula B. Kavathas^2,*,

^* Department of Laboratory Medicine and Department of Genetics and Section of Immunobiology, Yale University, New Haven, CT 06520; Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, Bronx, NY 10461; and Lawrence Berkeley Laboratory, University of California, Berkeley, CA 94720

To locate elements regulating the human CD8 gene complex, we mapped nuclear matrix attachment regions (MARs) and DNase I hypersensitive (HS) sites over a 100-kb region that included the CD8B gene, the intergenic region, and the CD8A gene. MARs facilitate long-range chromatin remodeling required for enhancer activity and have been found closely linked to several lymphoid enhancers. Within the human CD8 gene complex, we identified six DNase HS clusters, four strong MARs, and several weaker MARs. Three of the strong MARs were closely linked to two tissue-specific DNase HS clusters (III and IV) at the 3' end of the CD8B gene. To further establish the importance of this region, we obtained 19 kb of sequence and screened for potential binding sites for the MAR-binding protein, SATB1, and for GATA-3, both of which are critical for T cell development. By gel shift analysis we identified two strong SATB1 binding sites, located 4.5 kb apart, in strong MARs. We also detected strong GATA-3 binding to an oligonucleotide containing two GATA-3 motifs located at an HS site in cluster IV. This clustering of DNase HS sites and MARs capable of binding SATB1 and GATA-3 at the 3' end of the CD8B gene suggests that this region is an epigenetic regulator of CD8 expression.

This article has been cited by other articles:

				H. Nie, S. D. Maika, P. W. Tucker, and P. D. Gottlieb A Role for SATB1, a Nuclear Matrix Association Region-Binding Protein, in the Development of CD8SP Thymocytes and Peripheral T Lymphocytes J. Immunol., April 15, 2005; 174(8): 4745 - 4752. [Abstract] [Full Text] [PDF]

				T.-Y. Roh, S. Cuddapah, and K. Zhao Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping Genes & Dev., March 1, 2005; 19(5): 542 - 552. [Abstract] [Full Text] [PDF]

				R. P. Martins, G. C. Ostermeier, and S. A. Krawetz Nuclear Matrix Interactions at the Human Protamine Domain: A WORKING MODEL OF POTENTIATION J. Biol. Chem., December 10, 2004; 279(50): 51862 - 51868. [Abstract] [Full Text] [PDF]

				Y. Huang and D. Kowalski WEB-THERMODYN: sequence analysis software for profiling DNA helical stability Nucleic Acids Res., July 1, 2003; 31(13): 3819 - 3821. [Abstract] [Full Text] [PDF]

				P. Goebel, A. Montalbano, N. Ayers, E. Kompfner, L. Dickinson, C. F. Webb, and A. J. Feeney High Frequency of Matrix Attachment Regions and Cut-Like Protein x/CCAAT-Displacement Protein and B Cell Regulator of IgH Transcription Binding Sites Flanking Ig V Region Genes J. Immunol., September 1, 2002; 169(5): 2477 - 2487. [Abstract] [Full Text] [PDF]