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* Blood Research Institute, Blood Center of Southeastern Wisconsin, Milwaukee, WI 53201;
Department of Immunology, University of Washington, Seattle, WA 98105; and
Howard Hughes Medical Institute, National Jewish Medical and Research Center, Denver, CO 80206
The generation of the naive T cell repertoire is a direct result of
maturation and selection events in the thymus. Although maturation
events are judged predominantly on the expression of surface markers,
molecular markers, more intimately involved in the selection process,
can be informative. We have identified a molecular marker for selection
in later stages of maturation in humans. Thymocytes are selected for
the expression of TCR 
-chains with shorter CDR3 at the
double-positive to single-positive (SP) transition. Here we extend
these studies to the mouse and show that the selection phenotype is not
related to 
-chain pairing but is a function of the MHC haplotype.
Interestingly, the selection is much more apparent in CD4 SP thymocytes
than in CD8 SP cells. This is in contrast to human thymocytes, where
the selection is equally apparent in both lineages. The involvement of
MHC in the process argues that this is a positive selection stage. The
difference in the extent of this selection between the two SP lineages
may indicate a class difference in the nature of the TCR-MHC
interaction, the role of coreceptors in the selection process, or
both.
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