| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Institute for Advanced Medical Research and
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan; and
Department of Internal Medicine, Tokyo Electric Power Company Hospital, Tokyo, Japan
We have recently reported that in patients with chronic immune thrombocytopenic purpura (IMTP), circulating T and B cells that are responsive to gpIIb-IIIa can induce anti-platelet autoantibody production. In this study, the frequencies and activation status of gpIIb-IIIa-reactive T and B cells were evaluated in the peripheral blood and spleen obtained from nine IMTP patients undergoing splenectomy. There was no difference in gpIIb-IIIa-reactive T cell frequencies between peripheral blood and spleen (6.4 ± 2.6 vs 5.2 ± 2.4 per 105 T cells), as determined by limiting dilution analysis, but activated T cells responsive to gpIIb-IIIa showing accelerated proliferation kinetics and those expressing CD154 were more frequent in spleen than in peripheral blood. The frequencies of anti-gpIIb-IIIa Ab-producing B cells, as determined by ELISPOT assay, were also similar in peripheral blood and spleen (61.2 ± 24.0 vs 77.7 ± 45.3 per 105 B cells); however, an anti-gpIIb-IIIa Ab was spontaneously produced by splenocytes in vitro, but scarcely secreted by PBMCs. CD19-/surface Ig-/CD38+/CD138+ plasma cells secreting anti-gpIIb-IIIa Ab were exclusively detected in the spleen. In serial analysis, the frequencies of circulating gpIIb-IIIa-reactive T and B cells were markedly decreased after splenectomy in patients with a complete response, but were unchanged in nonresponders. These findings indicate that an interaction between gpIIb-IIIa-reactive T and B cells inducing anti-platelet Ab production in IMTP patients occurs primarily in the spleen and that the significant number of gpIIb-IIIa-reactive T and B cells activated in the spleen are released into the circulation as memory cells.
This article has been cited by other articles:
|
|
 |
|
  Y. Yamaguchi, N. Seta, J. Kaburaki, K. Kobayashi, E. Matsuura, and M. Kuwana Excessive exposure to anionic surfaces maintains autoantibody response to 2-glycoprotein I in patients with antiphospholipid syndrome Blood, December 15, 2007; 110(13): 4312 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Emilia, M. Luppi, P. Zucchini, M. Morselli, L. Potenza, F. Forghieri, F. Volzone, G. Jovic, G. Leonardi, A. Donelli, et al. Helicobacter pylori infection and chronic immune thrombocytopenic purpura: long-term results of bacterium eradication and association with bacterium virulence profiles Blood, December 1, 2007; 110(12): 3833 - 3841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sukati, H. G. Watson, S. J. Urbaniak, and R. N. Barker Mapping helper T-cell epitopes on platelet membrane glycoprotein IIIa in chronic autoimmune thrombocytopenic purpura Blood, May 15, 2007; 109(10): 4528 - 4538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sakakura, H. Wada, Y. Abe, J. Nishioka, H. Tomatsu, Y. Hamaguchi, S. Oguni, H. Shiku, and T. Nobori Usefulness of Measurement of Reticulated Platelets for Diagnosis of Idiopathic Thrombocytopenic Purpura Clinical and Applied Thrombosis/Hemostasis, July 1, 2005; 11(3): 253 - 261. [Abstract] [PDF]
|
|
|
|
|
|
M. Kuwana, E. Matsuura, K. Kobayashi, Y. Okazaki, J. Kaburaki, Y. Ikeda, and Y. Kawakami Binding of {beta}2-glycoprotein I to anionic phospholipids facilitates processing and presentation of a cryptic epitope that activates pathogenic autoreactive T cells Blood, February 15, 2005; 105(4): 1552 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Solanilla, J.-M. Pasquet, J.-F. Viallard, C. Contin, C. Grosset, J. Dechanet-Merville, M. Dupouy, M. Landry, F. Belloc, P. Nurden, et al. Platelet-associated CD154 in immune thrombocytopenic purpura Blood, January 1, 2005; 105(1): 215 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P. Panitsas, M. Theodoropoulou, A. Kouraklis, M. Karakantza, G. L. Theodorou, N. C. Zoumbos, A. Maniatis, and A. Mouzaki Adult chronic idiopathic thrombocytopenic purpura (ITP) is the manifestation of a type-1 polarized immune response Blood, April 1, 2004; 103(7): 2645 - 2647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kuwana, S. Nomura, K. Fujimura, T. Nagasawa, Y. Muto, Y. Kurata, S. Tanaka, and Y. Ikeda Effect of a single injection of humanized anti-CD154 monoclonal antibody on the platelet-specific autoimmune response in patients with immune thrombocytopenic purpura Blood, February 15, 2004; 103(4): 1229 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kuwana, Y. Kawakami, and Y. Ikeda Suppression of autoreactive T-cell response to glycoprotein IIb/IIIa by blockade of CD40/CD154 interaction: implications for treatment of immune thrombocytopenic purpura Blood, January 15, 2003; 101(2): 621 - 623. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
