| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Experimental Biology and Pharmacology, Serono Pharmaceutical Research Institute, Geneva, Switzerland;
Laboratory of Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands;
InterPharma Laboratories, Nes Ziona, Israel;
Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, CO 80262; and
¶ Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
The pathogenesis of Crohn’s disease (CD) remains under intense investigation. Increasing evidence suggests a role for mature IL-18 in the induction of proinflammatory cytokines and Th1 polarization in CD lesions. The aim of this study was to investigate the contribution of the IL-18-neutralizing (a and c) and non-neutralizing (b and d) isoforms of IL-18-binding protein (IL-18BP) during active CD. Intestinal endothelial cells and macrophages were the major source of IL-18BP within the submucosa, and this IL-18BP production was also found to be relevant to other types of endothelial cells (HUVEC) and macrophages (peripheral monocytes). IL-18BP messenger transcript and protein were significantly increased in surgically resected specimens from active CD compared with control patients, correlating with an up-regulation of IL-18. Analysis of the expression of the four IL-18BP isoforms as well as being free or bound to IL-18 was reported and revealed that unbound IL-18BP isoforms a and c and inactive isoform d were present in specimens from active CD and control patients while isoform b was not detected. IL-18/IL-18BP complex was also detected. Interestingly, although most was complexed, free mature IL-18 could still be detected in active CD specimens even in the presence of the IL-18BP isoform a/c. These results demonstrate that the appropriate neutralizing isoforms are present in the intestinal tissue of patients with active CD and highlights the complexity of IL-18/IL-18BP biology.
This article has been cited by other articles:
|
|
 |
|
  M. Pietropaolo, J. M. Surhigh, P. W. Nelson, and G. S. Eisenbarth Primer: Immunity and Autoimmunity Diabetes, November 1, 2008; 57(11): 2872 - 2882. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schif-Zuck, J. Westermann, N. Netzer, Y. Zohar, M. Meiron, G. Wildbaum, and N. Karin Targeted Overexpression of IL-18 Binding Protein at the Central Nervous System Overrides Flexibility in Functional Polarization of Antigen-Specific Th2 Cells J. Immunol., April 1, 2005; 174(7): 4307 - 4315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kammerer, R. B. Roth, C. R. Hoyal, R. Reneland, G. Marnellos, M. Kiechle, U. Schwarz-Boeger, L. R. Griffiths, F. Ebner, J. Rehbock, et al. Association of the NuMA region on chromosome 11q13 with breast cancer susceptibility PNAS, February 8, 2005; 102(6): 2004 - 2009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gatto, C. Berlato, V. Poli, S. Tininini, I. Kinjyo, A. Yoshimura, M. A. Cassatella, and F. Bazzoni Analysis of SOCS-3 Promoter Responses to Interferon {gamma} J. Biol. Chem., April 2, 2004; 279(14): 13746 - 13754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Plitz, P. Saint-Mezard, M. Satho, S. Herren, C. Waltzinger, M. de Carvalho Bittencourt, M. H. Kosco-Vilbois, and Y. Chvatchko IL-18 Binding Protein Protects Against Contact Hypersensitivity J. Immunol., August 1, 2003; 171(3): 1164 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Moller, J. Paulukat, M. Nold, M. Behrens, N. Kukoc-Zivojnov, J. P. Kaltwasser, J. Pfeilschifter, and H. Muhl Interferon-{gamma} induces expression of interleukin-18 binding protein in fibroblast-like synoviocytes Rheumatology, March 1, 2003; 42(3): 442 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. K. Banda, A. Vondracek, D. Kraus, C. A. Dinarello, S.-H. Kim, A. Bendele, G. Senaldi, and W. P. Arend Mechanisms of Inhibition of Collagen-Induced Arthritis by Murine IL-18 Binding Protein J. Immunol., February 15, 2003; 170(4): 2100 - 2105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Gracie, S. E. Robertson, and I. B. McInnes Interleukin-18 J. Leukoc. Biol., February 1, 2003; 73(2): 213 - 224. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
