| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
in the Small Spotted Catshark (Scyliorhinus canicula)1
*
Department of Zoology, University of Aberdeen, Aberdeen, United Kingdom; and
Sars International Center for Marine Molecular Biology, Bergen, Norway
Cartilaginous fish are considered the most primitive living
jawed vertebrates with a complex immune system typical of all jawed
vertebrates. Cytokine homologs are found within jawless and bony fish,
although no cytokine or cytokine receptor genes have been sequenced in
cartilaginous fish. In this study the complete coding sequence of the
small spotted catshark (Scyliorhinus canicula) IL-1
gene is presented that contains a short 5' untranslated region
(54 bp), a 903-bp open reading frame, a 379-bp 3' untranslated region,
a polyadenylation signal, and eight mRNA instability motifs. The
predicted translation (301 amino acids) has highest identity to trout
IL-1 (31.7%), with greatest homology within the putative 12
-sheets. The IL-1 family signature is also present, but there is no
apparent signal peptide. As with other nonmammalian IL-1 sequences,
the IL-1-converting enzyme cut site is absent. Expression of the
IL-1 transcript is detectable by RT-PCR in the spleen and testes,
induced in vivo with LPS. Furthermore, a 7-fold increase of transcript
levels in splenocytes incubated for 5 h with LPS was seen. The
genomic organization comprises six exons and five introns with highest
homology seen in exons encoding the largest amount of secondary
structure per amino acid. Southern blot analysis suggests at least two
copies of the IL-1 gene or genes related to the 3' end of the
IL-1 sequence are present in the catshark. The cloning of IL-1 in
S. canicula, the first cytokine sequenced within
cartilaginous fish, verifies previous bioactivity evidence for the
presence of inflammatory cytokines.
This article has been cited by other articles:
|
|
 |
|
  K. L. Hailey, S. Li, M. D. Andersen, M. Roy, V. L. Woods Jr, and P. A. Jennings Pro-interleukin (IL)-1{beta} Shares a Core Region of Stability as Compared with Mature IL-1{beta} While Maintaining a Distinctly Different Configurational Landscape: A COMPARATIVE HYDROGEN/DEUTERIUM EXCHANGE MASS SPECTROMETRY STUDY J. Biol. Chem., September 18, 2009; 284(38): 26137 - 26148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Wang, S. Bird, A. Koussounadis, J. W. Holland, A. Carrington, J. Zou, and C. J. Secombes Identification of a Novel IL-1 Cytokine Family Member in Teleost Fish J. Immunol., July 15, 2009; 183(2): 962 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Coolen, A. Menuet, D. Chassoux, C. Compagnucci, S. Henry, L. Leveque, C. Da Silva, F. Gavory, S. Samain, P. Wincker, et al. The Dogfish Scyliorhinus canicula: A Reference in Jawed Vertebrates CSH Protocols, December 1, 2008; 2008(13): pdb.emo111 - pdb.emo111. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. J. Walsh, C. A. Luer, A.B. Bodine, C. A. Smith, H. L. Cox, D. R. Noyes, and M. Gasparetto Elasmobranch immune cells as a source of novel tumor cell inhibitors: Implications for public health Integr. Comp. Biol., December 1, 2006; 46(6): 1072 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Gobel, K. Schneider, B. Schaerer, I. Mejri, F. Puehler, S. Weigend, P. Staeheli, and B. Kaspers IL-18 Stimulates the Proliferation and IFN-{gamma} Release of CD4+ T Cells in the Chicken: Conservation of a Th1-Like System in a Nonmammalian Species J. Immunol., August 15, 2003; 171(4): 1809 - 1815. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
