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The Journal of Immunology, 2002, 168: 3295-3302.
Copyright © 2002 by The American Association of Immunologists

Expression of Inhibitory Receptors Ly49E and CD94/NKG2 on Fetal Thymic and Adult Epidermal TCR V{gamma}3 Lymphocytes1

Katrien Van Beneden, An De Creus, Frederik Stevenaert, Veronique Debacker, Jean Plum and Georges Leclercq2

Department of Clinical Chemistry, Microbiology, and Immunology, University of Ghent, University Hospital, Ghent, Belgium

Ly49 and CD94/NKG2 inhibitory receptors are predominantly expressed on murine NK cells, but they are also expressed on a subpopulation of peripheral CD8 memory TCR {alpha}{beta} lymphocytes. In this study we demonstrate that Ly49E and CD94/NKG2 receptors are expressed on mature TCR V{gamma}3+ cells in the fetal thymus. Expression correlated with a memory phenotype, such as expression of CD44, 2B4, and IL-2R{beta} (CD122), and absence of IL-2R{alpha} (CD25) expression. No expression of Ly49A, C, D, G2, or I receptors was observed. This phenotype is similar to that of fetal thymic NK cells. Skin-located V{gamma}3 T cells, the progeny of fetal thymic V{gamma}3 cells, also expressed CD94/NKG2 and Ly49E but not the other members of the Ly49 family. The development and survival of Ly49E+ or CD94/NKG2+ V{gamma}3 T lymphocytes was not dependent upon expression of MHC class I molecules. The cytotoxicity of TCR V{gamma}3 cells was inhibited when Qdm, the ligand for CD94/NKG2, was presented by Qa1b-transfected target cells. Also, upon cross-linking of CD94/NKG2 with mAb 3S9, TCR V{gamma}3 thymocytes were prevented from killing Fc{gamma}R+ P815 target cells. These effects were most pronounced in the CD94/NKG2high subpopulation as compared with the CD94/NKG2low subpopulation of V{gamma}3 cells. Our data demonstrate that V{gamma}3 T cells expressing inhibitory Ly49E and CD94/NKG2 receptors are mature and display a memory phenotype, and that CD94/NKG2 functions as an inhibitory receptor on these T lymphocytes.




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